################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII AccI AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoT22I FokI FseI FspI HgaI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MslI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 597 GGTACC G^GTACC AccII 18 CGCG CG^CG AcsI 269 GAATTT [GA]^AATT[CT] AflII 942 CTTAAG C^TTAAG AlwNI 844 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 269 GAATTT [GA]^AATT[CT] AseI 496 ATTAAT AT^TAAT AsnI 496 ATTAAT AT^TAAT Asp718I 597 GGTACC G^GTACC AspHI 21 GAGCTC G[AT]GC[AT]^C AvaII 664 GGACC G^G[AT]CC BalI 563 TGGCCA TGG^CCA BciVI 332 GTATCC GTATCC(6/5) BclI 441 TGATCA T^GATCA BfaI 418 CTAG C^TAG BfrI 942 CTTAAG C^TTAAG BglII 414 AGATCT A^GATCT BsiEI 64 CGGCCG CG[GA][CT]^CG BsiHKAI 21 GAGCTC G[AT]GC[AT]^C BsmAI 829 GTCTC GTCTC(1/5) BspHI 522 TCATGA T^CATGA BspLU11I 825 ACATGT A^CATGT BsrFI 39 GCCGGC [GA]^CCGG[CT] BssSI 151 CACGAG CACGAG(-5/-1) BstUI 18 CGCG CG^CG Cfr10I 39 GCCGGC [GA]^CCGG[CT] DraII 663 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 713 CCGTGG C^C[GA][CT]GG EagI 64 CGGCCG C^GGCCG EarI 156 GAAGAG CTCTTC(1/4) EclXI 64 CGGCCG C^GGCCG Eco52I 64 CGGCCG C^GGCCG EcoO109I 663 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 206 CCAAGG C^C[AT][AT]GG FbaI 441 TGATCA T^GATCA KpnI 597 GGTACC GGTAC^C Ksp632I 156 GAAGAG CTCTTC(1/4) MaeI 418 CTAG C^TAG MaeII 929 ACGT A^CGT MluNI 563 TGGCCA TGG^CCA MscI 563 TGGCCA TGG^CCA MspA1I 61 CAGCGG C[AC]G^C[GT]G MvnI 18 CGCG CG^CG NaeI 39 GCCGGC GCC^GGC NgoMIV 39 GCCGGC G^CCGGC NotI 63 GCGGCCGC GC^GGCCGC NspI 825 ACATGT [GA]CATG^[CT] PpuMI 663 AGGACCT [GA]G^G[AT]CC[CT] PshBI 496 ATTAAT AT^TAAT RcaI 522 TCATGA T^CATGA SacI 21 GAGCTC GAGCT^C SapI 156 GAAGAGC GCTCTTC(1/4) SfaNI 470 GCATC GCATC(5/9) SmlI 942 CTTAAG C^T[CT][GA]AG StyI 206 CCAAGG C^C[AT][AT]GG TaqI 697 TCGA T^CGA TfiI 490 GATTC G^A[AT]TC Tsp45I 778 GTGAC ^GT[GC]AC TthHB8I 697 TCGA T^CGA XbaI 417 TCTAGA T^CTAGA XcmI 602 CCATTCCAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 36 GGCGCC G[GA]^CG[CT]C 127 GGCGCC AfaI 584 GTAC GT^AC 598 GTAC AflIII 825 ACATGT A^C[GA][CT]GT 929 ACGTGT AlwI 222 GGATC GGATC(4/5) 886 GGATC AvaI 24 CTCGGG C^[CT]CG[GA]G 121 CTCGGG BanII 13 GAGCCC G[GA]GC[CT]^C 21 GAGCTC BbeI 36 GGCGCC GGCGC^C 127 GGCGCC BbsI 526 GAAGAC GAAGAC(2/6) 921 GTCTTC BcnI 131 CCGGG CC^[GC]GG 960 CCCGG BglI 39 GCCGGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 565 GCCATTCAGGC BmyI 13 GAGCCC G[AGT]GC[ACT]^C 21 GAGCTC BpmI 773 CTCCAG CTGGAG(16/14) 592 CTGGAG BpuAI 526 GAAGAC GAAGAC(2/6) 921 GTCTTC BsaHI 36 GGCGCC G[GA]^CG[CT]C 127 GGCGCC BsiYI 115 CCGCAGCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 607 CCAGAAATGGG BslI 115 CCGCAGCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 607 CCAGAAATGGG BsoBI 24 CTCGGG C^[CT]CG[GA]G 121 CTCGGG Bsp1286I 13 GAGCCC G[AGT]GC[ACT]^C 21 GAGCTC BstNI 162 CCTGG CC^[AT]GG 843 CCAGG EaeI 64 CGGCCG [CT]^GGCC[GA] 563 TGGCCA EcoRII 162 CCTGG ^CC[AT]GG 843 CCAGG HaeII 36 GGCGCC [GA]GCGC^[CT] 127 GGCGCC Hin1I 36 GGCGCC G[GA]^CG[CT]C 127 GGCGCC HphI 550 GGTGA GGTGA(8/7) 281 TCACC KasI 36 GGCGCC G^GCGCC 127 GGCGCC MaeIII 517 GTAAC ^GT[ACGT]AC 778 GTGAC MvaI 162 CCTGG CC^[AT]GG 843 CCAGG NarI 36 GGCGCC GG^CGCC 127 GGCGCC NciI 131 CCGGG CC^[GC]GG 960 CCCGG PstI 196 CTGCAG CTGCA^G 850 CTGCAG RsaI 584 GTAC GT^AC 598 GTAC SfoI 36 GGCGCC GGC^GCC 127 GGCGCC TspRI 337 CAGTG CA[GC]TG[ACGT][ACGT]^ 776 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI BanI BbvI BsaJI BsrI BssKI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRV FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MflI MnlI MseI MspI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI Tru9I TseI Tsp509I XhoII