################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspHI AspI BalI BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FauI FbaI FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MluI MluNI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 2564 GTCTAC GT^[AC][GT]AC AccIII 1045 TCCGGA T^CCGGA AciI 2251 CCGC CCGC(-3/-1) AhdI 418 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 904 GGGCCC GGGCC^C AspEI 418 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 99 TGCGCA TGC^GCA AvrII 247 CCTAGG C^CTAGG BanII 904 GGGCCC G[GA]GC[CT]^C BbsI 2175 GTCTTC GAAGAC(2/6) BglII 370 AGATCT A^GATCT BlnI 247 CCTAGG C^CTAGG BlpI 1543 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1543 GCTCAGC GC^T[ACGT]AGC BpuAI 2175 GTCTTC GAAGAC(2/6) BsaI 156 GAGACC GGTCTC(1/5) BsaWI 1045 TCCGGA [AT]^CCGG[AT] BseAI 1045 TCCGGA T^CCGGA BsgI 817 GTGCAG GTGCAG(16/14) BsmI 2126 GCATTC GAATGC(1/-1) BspEI 1045 TCCGGA T^CCGGA BspHI 2234 TCATGA T^CATGA BssSI 20 CTCGTG CACGAG(-5/-1) BstXI 1713 CCATAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1543 GCTCAGC GC^T[ACGT]AGC CfoI 100 GCGC GCG^C DrdI 1805 GACCAAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 418 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1159 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2321 ATGCAT ATGCA^T FspI 99 TGCGCA TGC^GCA HapII 1046 CCGG C^CGG HhaI 100 GCGC GCG^C HinP1I 100 GCGC G^CGC HpaII 1046 CCGG C^CGG MfeI 1681 CAATTG C^AATTG MroI 1045 TCCGGA T^CCGGA MslI 2229 CACTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1046 CCGG C^CGG MunI 1681 CAATTG C^AATTG MwoI 1983 GCTGGAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NsiI 2321 ATGCAT ATGCA^T PaeR7I 1537 CTCGAG C^TCGAG PshAI 1018 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 569 CTGCAG CTGCA^G RcaI 2234 TCATGA T^CATGA SspI 1384 AATATT AAT^ATT Tsp45I 1325 GTGAC ^GT[GC]AC XbaI 1071 TCTAGA T^CTAGA XhoI 1537 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflIII 870 ACATGT A^C[GA][CT]GT 2448 ACATGT AlwI 1450 GATCC GGATC(4/5) 1822 GATCC Asp700I 2097 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2335 GAAAGTATTC AvaI 227 CCCGAG C^[CT]CG[GA]G 1537 CTCGAG BbvI 1153 GCAGC GCAGC(8/12) 4 GCTGC BciVI 729 GGATAC GTATCC(6/5) 735 GGATAC BmyI 904 GGGCCC G[AGT]GC[ACT]^C 2658 GGGCAC BpmI 1633 CTCCAG CTGGAG(16/14) 2522 CTCCAG BsaBI 137 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1444 GATGATGATC BsoBI 227 CCCGAG C^[CT]CG[GA]G 1537 CTCGAG Bsp1286I 904 GGGCCC G[AGT]GC[ACT]^C 2658 GGGCAC BspLU11I 870 ACATGT A^CATGT 2448 ACATGT BspMI 1797 ACCTGC ACCTGC(4/8) 819 GCAGGT BsrDI 96 CATTGC GCAATG(2/0) 2506 CATTGC BstYI 370 AGATCT [GA]^GATC[CT] 1821 AGATCC DraII 903 AGGGCCC [GA]G^G[ACGT]CC[CT] 904 GGGCCCT EcoO109I 903 AGGGCCC [GA]G^G[ACGT]CC[CT] 904 GGGCCCT EcoT14I 247 CCTAGG C^C[AT][AT]GG 1719 CCTTGG Fnu4HI 4 GCTGC GC^[ACGT]GC 1153 GCAGC HaeIII 557 GGCC GG^CC 905 GGCC HgaI 1916 GACGC GACGC(5/10) 2030 GACGC ItaI 4 GCTGC GC^[ACGT]GC 1153 GCAGC MamI 137 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1444 GATGATGATC MflI 370 AGATCT [GA]^GATC[CT] 1821 AGATCC MspA1I 629 CAGCTG C[AC]G^C[GT]G 880 CAGCTG NspI 870 ACATGT [GA]CATG^[CT] 2448 ACATGT PvuII 629 CAGCTG CAG^CTG 880 CAGCTG StyI 247 CCTAGG C^C[AT][AT]GG 1719 CCTTGG TseI 4 GCTGC G^C[AT]GC 1153 GCAGC XcmI 1004 CCAATAACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1613 CCATTGGATTTGTGG XhoII 370 AGATCT [GA]^GATC[CT] 1821 AGATCC XmnI 2097 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2335 GAAAGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AcsI AfaI AluI AlwNI ApoI AvaII BanI BfaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII FokI HinfI HphI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I