(Linear) (Six-base) MAPSORT of: KIEE1288.seq Check: 5110 from: 1 to: 2706 KIEE1288.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AccI GT'mk_AC Cuts at: 0 2565 2706 Size: 2565 141 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 128 254 395 2033 2398 2706 Size: 128 126 141 1638 365 308 Fragments arranged by size: 1638 365 308 141 128 126 AflIII A'CryG_T Cuts at: 0 870 2448 2706 Size: 870 1578 258 Fragments arranged by size: 1578 870 258 AhdI GACnn_n'nnGTC Cuts at: 0 423 2706 Size: 423 2283 AlwNI CAG_nnn'CTG Cuts at: 0 166 331 718 2706 Size: 166 165 387 1988 Fragments arranged by size: 1988 387 166 165 ApaI G_GGCC'C Cuts at: 0 908 2706 Size: 908 1798 ApoI r'AATT_y Cuts at: 0 1295 1725 1738 2358 2706 Size: 1295 430 13 620 348 Fragments arranged by size: 1295 620 430 348 13 AvaI C'yCGr_G Cuts at: 0 227 1537 2706 Size: 227 1310 1169 Fragments arranged by size: 1310 1169 227 AvrII C'CTAG_G Cuts at: 0 247 2706 Size: 247 2459 BanI G'GyrC_C Cuts at: 0 795 1586 2659 2706 Size: 795 791 1073 47 Fragments arranged by size: 1073 795 791 47 BanII G_rGCy'C Cuts at: 0 908 2706 Size: 908 1798 BbsI GAAGACnn'nnnn_ Cuts at: 0 2168 2706 Size: 2168 538 BciVI GTATCCnnnnn_n' Cuts at: 0 723 729 2706 Size: 723 6 1977 Fragments arranged by size: 1977 723 6 BfrBI ATG'CAT Cuts at: 0 2323 2706 Size: 2323 383 BglII A'GATC_T Cuts at: 0 370 2706 Size: 370 2336 BlpI GC'TnA_GC Cuts at: 0 1544 2706 Size: 1544 1162 Bme1580I G_kGCm'C Cuts at: 0 908 2662 2706 Size: 908 1754 44 Fragments arranged by size: 1754 908 44 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1618 2507 2706 Size: 1618 889 199 Fragments arranged by size: 1618 889 199 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 1048 1097 1538 1872 2706 Size: 113 935 49 441 334 834 Fragments arranged by size: 935 834 441 334 113 49 BsaI GGTCTCn'nnnn_ Cuts at: 0 150 2706 Size: 150 2556 BsaBI GATnn'nnATC Cuts at: 0 141 1448 2706 Size: 141 1307 1258 Fragments arranged by size: 1307 1258 141 BsaWI w'CCGG_w Cuts at: 0 1045 2706 Size: 1045 1661 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 453 958 1231 2706 Size: 453 505 273 1475 Fragments arranged by size: 1475 505 453 273 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 838 2706 Size: 838 1868 BsmI GAATG_Cn' Cuts at: 0 2126 2706 Size: 2126 580 Bsp1286I G_dGCh'C Cuts at: 0 908 2662 2706 Size: 908 1754 44 Fragments arranged by size: 1754 908 44 BspEI T'CCGG_A Cuts at: 0 1045 2706 Size: 1045 1661 BspHI T'CATG_A Cuts at: 0 2234 2706 Size: 2234 472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 810 1806 2706 Size: 810 996 900 Fragments arranged by size: 996 900 810 BsrDI GCAATG_nn' Cuts at: 0 95 2505 2706 Size: 95 2410 201 Fragments arranged by size: 2410 201 95 BssSI C'ACGA_G Cuts at: 0 20 2706 Size: 20 2686 BstXI CCAn_nnnn'nTGG Cuts at: 0 1720 2706 Size: 1720 986 BstYI r'GATC_y Cuts at: 0 370 1821 2706 Size: 370 1451 885 Fragments arranged by size: 1451 885 370 BtsI GCAGTG_nn' Cuts at: 0 978 994 2306 2706 Size: 978 16 1312 400 Fragments arranged by size: 1312 978 400 16 DraI TTT'AAA Cuts at: 0 212 347 1743 2391 2706 Size: 212 135 1396 648 315 Fragments arranged by size: 1396 648 315 212 135 DrdI GACnn_nn'nnGTC Cuts at: 0 1811 2706 Size: 1811 895 EarI CTCTTCn'nnn_ Cuts at: 0 123 148 1430 1761 2706 Size: 123 25 1282 331 945 Fragments arranged by size: 1282 945 331 123 25 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 128 254 395 1618 2033 2398 2507 2706 Size: 128 126 141 1223 415 365 109 199 Fragments arranged by size: 1223 415 365 199 141 128 126 109 EcoNI CCTnn'n_nnAGG Cuts at: 0 1163 2706 Size: 1163 1543 EcoO109I rG'GnC_Cy Cuts at: 0 904 905 2706 Size: 904 1 1801 Fragments arranged by size: 1801 904 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 577 2232 2706 Size: 577 1655 474 Fragments arranged by size: 1655 577 474 FspI TGC'GCA Cuts at: 0 101 2706 Size: 101 2605 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 409 441 1404 1436 1825 1857 1860 1892 Size: 409 32 963 32 389 32 3 32 Cuts at: 1892 2266 2298 2706 Size: 374 32 408 Fragments arranged by size: 963 409 408 389 374 32 32 32 32 32 3 MfeI C'AATT_G Cuts at: 0 1681 2706 Size: 1681 1025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 809 2706 Size: 809 1897 MslI CAynn'nnrTG Cuts at: 0 2233 2706 Size: 2233 473 MspA1I CmG'CkG Cuts at: 0 631 882 2706 Size: 631 251 1824 Fragments arranged by size: 1824 631 251 NsiI A_TGCA'T Cuts at: 0 2325 2706 Size: 2325 381 NspI r_CATG'y Cuts at: 0 874 2452 2706 Size: 874 1578 254 Fragments arranged by size: 1578 874 254 PciI A'CATG_T Cuts at: 0 870 2448 2706 Size: 870 1578 258 Fragments arranged by size: 1578 870 258 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1979 2011 2706 Size: 1979 32 695 Fragments arranged by size: 1979 695 32 PshAI GACnn'nnGTC Cuts at: 0 1022 2706 Size: 1022 1684 PsiI TTA'TAA Cuts at: 0 637 2706 Size: 637 2069 PspOMI G'GGCC_C Cuts at: 0 904 2706 Size: 904 1802 PspXI vC'TCGA_Gb Cuts at: 0 1537 2706 Size: 1537 1169 PstI C_TGCA'G Cuts at: 0 573 2706 Size: 573 2133 PvuII CAG'CTG Cuts at: 0 631 882 2706 Size: 631 251 1824 Fragments arranged by size: 1824 631 251 SfcI C'TryA_G Cuts at: 0 299 569 899 2706 Size: 299 270 330 1807 Fragments arranged by size: 1807 330 299 270 SmlI C'TyrA_G Cuts at: 0 128 1027 1112 1537 1553 1887 2706 Size: 128 899 85 425 16 334 819 Fragments arranged by size: 899 819 425 334 128 85 16 SspI AAT'ATT Cuts at: 0 1386 2706 Size: 1386 1320 StyI C'CwwG_G Cuts at: 0 247 1719 2706 Size: 247 1472 987 Fragments arranged by size: 1472 987 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2294 2706 Size: 2294 412 TatI w'GTAC_w Cuts at: 0 442 1533 2091 2706 Size: 442 1091 558 615 Fragments arranged by size: 1091 615 558 442 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1308 1340 2706 Size: 1308 32 1366 Fragments arranged by size: 1366 1308 32 XbaI T'CTAG_A Cuts at: 0 1071 2706 Size: 1071 1635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1011 1620 2706 Size: 1011 609 1086 Fragments arranged by size: 1086 1011 609 XhoI C'TCGA_G Cuts at: 0 1537 2706 Size: 1537 1169 XmnI GAAnn'nnTTC Cuts at: 0 2101 2339 2706 Size: 2101 238 367 Fragments arranged by size: 2101 367 238 Enzymes that do cut: AccI AcuI AflIII AhdI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI BciVI BfrBI BglII BlpI Bme1580I BpmI BpuEI BsaI BsaBI BsaWI BseRI BsgI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BssSI BstXI BstYI BtsI DraI DrdI EarI Eco57MI EcoNI EcoO109I FalI FspI Hin4I MfeI MmeI MslI MspA1I NsiI NspI PciI PpiI PshAI PsiI PspOMI PspXI PstI PvuII SfcI SmlI SspI StyI TaqII TatI TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BclI BglI BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaHI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII EaeI EagI EciI EcoICRI EcoRI EcoRV FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpuMI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XmaI ZraI