################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AviII AvrII BamHI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BsrDI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII ClaI DraI DraIII DrdI Eco47III EcoO65I EcoRV FauI FbaI FspI HindIII HpaI KpnI MfeI MflI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI PshBI PvuI SacI SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 95 CGCG CG^CG AclI 1753 AACGTT AA^CGTT AflIII 1079 ACACGT A^C[GA][CT]GT AhdI 2290 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1456 CCTGAGG CC^T[ACGT]AGG Asp700I 603 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2290 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2265 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1583 CTCGGG C^[CT]CG[GA]G BalI 1022 TGGCCA TGG^CCA BanII 2329 GAGCCC G[GA]GC[CT]^C BbeI 9 GGCGCC GGCGC^C BbrPI 2005 CACGTG CAC^GTG BbsI 603 GAAGAC GAAGAC(2/6) BbvCI 223 CCTCAGC CCTCAGC(-5/-2) BglI 58 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 603 GAAGAC GAAGAC(2/6) BsaAI 2005 CACGTG [CT]AC^GT[GA] BsaBI 444 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 45 GAGACG CGTCTC(1/5) BsoBI 1583 CTCGGG C^[CT]CG[GA]G BspHI 1538 TCATGA T^CATGA BssSI 1381 CACGAG CACGAG(-5/-1) BstAPI 2190 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 95 CGCG CG^CG BstXI 1847 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1456 CCTGAGG CC^T[ACGT]AGG CpoI 2288 CGGACCG CG^G[AT]CCG Eam1105I 2290 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1456 CCTGAGG CC^T[ACGT]AGG EcoNI 2196 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 218 GAATTC G^AATTC FseI 88 GGCCGGCC GGCCGG^CC HaeII 9 GGCGCC [GA]GCGC^[CT] HincII 933 GTCAAC GT[CT]^[GA]AC HindII 933 GTCAAC GT[CT]^[GA]AC KasI 9 GGCGCC G^GCGCC KspI 94 CCGCGG CCGC^GG MamI 444 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1022 TGGCCA TGG^CCA MscI 1022 TGGCCA TGG^CCA MvnI 95 CGCG CG^CG NarI 9 GGCGCC GG^CGCC NspI 1850 ACATGC [GA]CATG^[CT] PflFI 2265 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2005 CACGTG CAC^GTG PmlI 2005 CACGTG CAC^GTG Psp1406I 1753 AACGTT AA^CGTT RcaI 1538 TCATGA T^CATGA RsrII 2288 CGGACCG CG^G[AT]CCG SacII 94 CCGCGG CCGC^GG SapI 822 GAAGAGC GCTCTTC(1/4) ScaI 1391 AGTACT AGT^ACT SfoI 9 GGCGCC GGC^GCC Tth111I 2265 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 603 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 9 GGCGCC G[GA]^CG[CT]C 1178 GGCGTC BanI 9 GGCGCC G^G[CT][GA]CC 1959 GGTGCC BpmI 1711 CTCCAG CTGGAG(16/14) 29 CTGGAG BsaHI 9 GGCGCC G[GA]^CG[CT]C 1178 GGCGTC Bsp1407I 526 TGTACA T^GTACA 1133 TGTACA BsrBI 895 GAGCGG CCGCTC(-3/-3) 2083 GAGCGG BsrGI 526 TGTACA T^GTACA 1133 TGTACA DraII 146 GGGGCCT [GA]G^G[ACGT]CC[CT] 837 GGGGCCT EcoO109I 146 GGGGCCT [GA]G^G[ACGT]CC[CT] 837 GGGGCCT EcoT22I 1120 ATGCAT ATGCA^T 1760 ATGCAT HgaI 2013 GACGC GACGC(5/10) 1179 GCGTC Hin1I 9 GGCGCC G[GA]^CG[CT]C 1178 GGCGTC NaeI 34 GCCGGC GCC^GGC 89 GCCGGC NcoI 317 CCATGG C^CATGG 1293 CCATGG NgoMIV 34 GCCGGC G^CCGGC 89 GCCGGC NsiI 1120 ATGCAT ATGCA^T 1760 ATGCAT SexAI 1190 ACCAGGT A^CC[AT]GGT 1895 ACCAGGT SspBI 526 TGTACA T^GTACA 1133 TGTACA StuI 436 AGGCCT AGG^CCT 1650 AGGCCT TaqI 710 TCGA T^CGA 918 TCGA TfiI 741 GATTC G^A[AT]TC 1772 GATTC TthHB8I 710 TCGA T^CGA 918 TCGA XcmI 317 CCATGGAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 755 CCATTTCAGAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BbvI BciVI BcnI BfaI BmyI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EarI EclXI Eco52I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI Tru9I TseI Tsp45I Tsp509I TspRI