(Circular) (Six-base) MAPSORT of: pFN21AB5739.seq Check: 5425 from: 1 to: 7072 pFN21AB5739 7072 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4232 4232 Size: 7072 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4882 278 Size: 53 83 186 936 3346 2468 Fragments arranged by size: 3346 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4415 1937 Size: 2478 4594 Acc65I G'GTAC_C Cuts at: 4393 4393 Size: 7072 AclI AA'CG_TT Cuts at: 1563 3759 5199 5572 1563 Size: 2196 1440 373 3063 Fragments arranged by size: 3063 2196 1440 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2367 2945 3350 3358 3668 3842 5135 6519 Size: 735 578 405 8 310 174 1293 1384 Cuts at: 6519 6787 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1293 735 578 405 310 268 174 8 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6875 AflIII A'CryG_T Cuts at: 1203 3084 5971 6948 1203 Size: 1881 2887 977 1327 Fragments arranged by size: 2887 1881 1327 977 AgeI A'CCGG_T Cuts at: 5903 5903 Size: 7072 AhdI GACnn_n'nnGTC Cuts at: 4300 5801 4300 Size: 1501 5571 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7072 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7072 AlwNI CAG_nnn'CTG Cuts at: 2470 3284 3421 4021 4224 4242 4815 6387 2470 Size: 814 137 600 203 18 573 1572 3155 Fragments arranged by size: 3155 1572 814 600 573 203 137 18 ApaLI G'TGCA_C Cuts at: 5129 6285 5129 Size: 1156 5916 ApoI r'AATT_y Cuts at: 1493 2223 2304 2430 4381 4607 4759 6868 1493 Size: 730 81 126 1951 226 152 2109 1697 Fragments arranged by size: 2109 1951 1697 730 226 152 126 81 AseI AT'TA_AT Cuts at: 160 1032 5626 160 Size: 872 4594 1606 Fragments arranged by size: 4594 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7072 AvaI C'yCGr_G Cuts at: 1870 1943 3588 4397 1870 Size: 73 1645 809 4545 Fragments arranged by size: 4545 1645 809 73 BamHI G'GATC_C Cuts at: 4402 4769 4402 Size: 367 6705 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 3964 4393 5848 618 Size: 325 217 627 227 1950 429 1455 1842 Fragments arranged by size: 1950 1842 1455 627 429 325 227 217 BanII G_rGCy'C Cuts at: 1391 4338 4391 1391 Size: 2947 53 4072 Fragments arranged by size: 4072 2947 53 BbeI G_GCGC'C Cuts at: 2018 2018 Size: 7072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2615 928 Size: 971 716 5385 Fragments arranged by size: 5385 971 716 BbvCI CC'TCA_GC Cuts at: 2229 2229 Size: 7072 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5263 5297 1903 Size: 34 3326 34 3678 Fragments arranged by size: 3678 3326 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2519 2716 2767 4964 6174 2519 Size: 197 51 2197 1210 3417 Fragments arranged by size: 3417 2197 1210 197 51 BclI T'GATC_A Cuts at: 1548 1548 Size: 7072 BfrBI ATG'CAT Cuts at: 3127 3767 3127 Size: 640 6432 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2069 5683 Size: 107 122 71 631 605 337 60 3614 Cuts at: 5683 136 Size: 1525 Fragments arranged by size: 3614 1525 631 605 337 122 107 71 60 BglII A'GATC_T Cuts at: 7067 7067 Size: 7072 BlpI GC'TnA_GC Cuts at: 4489 4489 Size: 7072 Bme1580I G_kGCm'C Cuts at: 1790 2948 3969 5133 6289 1790 Size: 1158 1021 1164 1156 2573 Fragments arranged by size: 2573 1164 1158 1156 1021 BmgBI CAC'GTC Cuts at: 1318 3087 1318 Size: 1769 5303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3103 4803 5761 Size: 416 53 83 399 24 1686 1700 958 Cuts at: 5761 442 Size: 1753 Fragments arranged by size: 1753 1700 1686 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7072 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4255 4255 Size: 7072 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 3701 5732 1239 Size: 816 1646 2031 2579 Fragments arranged by size: 2579 2031 1646 816 Bpu10I CC'TnA_GC Cuts at: 2229 2229 Size: 7072 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2225 2282 2524 2854 3431 5197 6062 6360 2225 Size: 57 242 330 577 1766 865 298 2937 Fragments arranged by size: 2937 1766 865 577 330 298 242 57 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5735 882 Size: 617 4236 2219 Fragments arranged by size: 4236 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4012 4863 493 Size: 711 2808 851 2702 Fragments arranged by size: 2808 2702 851 711 BsaBI GATnn'nnATC Cuts at: 2453 7066 2453 Size: 4613 2459 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 3184 4879 5261 Size: 53 83 186 936 482 1169 1695 382 Cuts at: 5261 5896 275 Size: 635 1451 Fragments arranged by size: 1695 1451 1169 936 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5505 5903 6177 6324 6735 5505 Size: 398 274 147 411 5842 Fragments arranged by size: 5842 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4259 4289 1178 Size: 30 165 30 2856 30 3961 Fragments arranged by size: 3961 2856 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2301 3050 3080 4346 4382 1186 Size: 1042 73 749 30 1266 36 3876 Fragments arranged by size: 3876 1266 1042 749 73 36 30 BseYI C'CCAG_C Cuts at: 2027 3099 6275 6771 2027 Size: 1072 3176 496 2328 Fragments arranged by size: 3176 2328 1072 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2189 2231 4034 4187 1163 Size: 573 453 42 1803 153 4048 Fragments arranged by size: 4048 1803 573 453 153 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2044 2057 2095 2099 2149 4522 Size: 275 186 43 13 38 4 50 2373 Cuts at: 4522 5283 6311 1540 Size: 761 1028 2301 Fragments arranged by size: 2373 2301 1028 761 275 186 50 43 38 13 4 BsiHKAI G_wGCw'C Cuts at: 3048 3156 3669 3717 4131 5133 5218 6289 3048 Size: 108 513 48 414 1002 85 1071 3831 Fragments arranged by size: 3831 1071 1002 513 414 108 85 48 BsmI GAATG_Cn' Cuts at: 2883 3274 3767 3941 4583 4676 2883 Size: 391 493 174 642 93 5279 Fragments arranged by size: 5279 642 493 391 174 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2044 2044 Size: 7072 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2948 3048 3156 3669 3717 3969 4131 Size: 399 1158 100 108 513 48 252 162 Cuts at: 4131 4338 4391 5133 5218 6289 1391 Size: 207 53 742 85 1071 2174 Fragments arranged by size: 2174 1158 1071 742 513 399 252 207 162 108 100 85 53 48 BspEI T'CCGG_A Cuts at: 6735 6735 Size: 7072 BspHI T'CATG_A Cuts at: 3543 4961 6691 3543 Size: 1418 1730 3924 Fragments arranged by size: 3924 1730 1418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3529 4113 4182 4209 4232 4427 844 Size: 2685 584 69 27 23 195 3489 Fragments arranged by size: 3489 2685 584 195 69 27 23 BsrBI CCG'CTC Cuts at: 1394 1823 2902 4090 4959 1394 Size: 429 1079 1188 869 3507 Fragments arranged by size: 3507 1188 1079 869 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5567 5741 65 Size: 1183 4319 174 1396 Fragments arranged by size: 4319 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 2039 2094 2144 4296 5716 5903 Size: 110 207 22 55 50 2152 1420 187 Cuts at: 5903 1700 Size: 2869 Fragments arranged by size: 2869 2152 1420 207 187 110 55 50 22 BsrGI T'GTAC_A Cuts at: 96 2531 3138 96 Size: 2435 607 4030 Fragments arranged by size: 4030 2435 607 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7072 BssSI C'ACGA_G Cuts at: 3386 5132 6144 3386 Size: 1746 1012 4314 Fragments arranged by size: 4314 1746 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4201 794 Size: 3407 3665 BstBI TT'CG_AA Cuts at: 5876 5876 Size: 7072 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7072 BstXI CCAn_nnnn'nTGG Cuts at: 3859 3859 Size: 7072 BstYI r'GATC_y Cuts at: 1972 4402 4769 5154 5171 6623 6709 6721 7067 Size: 2430 367 385 17 1452 86 12 346 Cuts at: 7067 1972 Size: 1977 Fragments arranged by size: 2430 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3462 3462 Size: 7072 BtgI C'CryG_G Cuts at: 513 1065 2004 2099 2322 3298 513 Size: 552 939 95 223 976 4287 Fragments arranged by size: 4287 976 939 552 223 95 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6333 Fragments arranged by size: 6333 683 56 BtsI GCAGTG_nn' Cuts at: 4588 5382 5402 4588 Size: 794 20 6258 Fragments arranged by size: 6258 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7072 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2195 2200 2230 2235 529 Size: 35 1631 5 30 5 5366 Fragments arranged by size: 5366 1631 35 30 5 5 DraI TTT'AAA Cuts at: 4376 4376 Size: 7072 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6079 809 Size: 507 4763 1802 Fragments arranged by size: 4763 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2092 2096 Size: 54 1425 50 275 18 211 51 4 Cuts at: 2096 2146 3027 4519 5408 7037 8 Size: 50 881 1492 889 1629 43 Fragments arranged by size: 1629 1492 1425 889 881 275 211 54 51 50 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2041 2092 2096 2146 4519 1537 Size: 275 229 51 4 50 2373 4090 Fragments arranged by size: 4090 2373 275 229 51 50 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 2343 2822 2967 3506 3992 5002 1353 Size: 966 24 479 145 539 486 1010 3423 Fragments arranged by size: 3423 1010 966 539 486 479 145 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2097 3980 5659 6033 6179 387 Size: 1064 138 508 1883 1679 374 146 1280 Fragments arranged by size: 1883 1679 1280 1064 508 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2367 2945 3350 3358 3668 3701 Size: 393 423 312 578 405 8 310 33 Cuts at: 3701 3842 5135 5732 6519 6787 1239 Size: 141 1293 597 787 268 1524 Fragments arranged by size: 1524 1293 787 597 578 423 405 393 312 310 268 141 33 8 EcoNI CCTnn'n_nnAGG Cuts at: 4205 4205 Size: 7072 EcoO109I rG'GnC_Cy Cuts at: 2152 2843 2152 Size: 691 6381 EcoRI G'AATT_C Cuts at: 2223 4381 4759 2223 Size: 2158 378 4536 Fragments arranged by size: 4536 2158 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2518 2587 2632 2848 2891 3535 2518 Size: 69 45 216 43 644 6055 Fragments arranged by size: 6055 644 216 69 45 43 FseI GG_CCGG'CC Cuts at: 2098 2098 Size: 7072 FspI TGC'GCA Cuts at: 5578 5578 Size: 7072 HaeII r_GCGC'y Cuts at: 1678 2018 6219 1678 Size: 340 4201 2531 Fragments arranged by size: 4201 2531 340 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2474 2506 3169 Size: 32 135 32 620 32 250 32 663 Cuts at: 3169 3201 3686 3718 3914 3946 4259 4286 4291 Size: 32 485 32 196 32 313 27 5 Cuts at: 4291 4318 5713 5745 5787 5819 1373 Size: 27 1395 32 42 32 2626 Fragments arranged by size: 2626 1395 663 620 485 313 250 196 135 42 32 32 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2940 4416 4662 669 Size: 988 281 1002 1476 246 3079 Fragments arranged by size: 3079 1476 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7072 HpaI GTT'AAC Cuts at: 4662 4662 Size: 7072 KasI G'GCGC_C Cuts at: 2014 2014 Size: 7072 KpnI G_GTAC'C Cuts at: 4397 4397 Size: 7072 MfeI C'AATT_G Cuts at: 4671 4671 Size: 7072 MluI A'CGCG_T Cuts at: 6948 6948 Size: 7072 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2695 2949 3876 6186 6370 1305 Size: 1390 254 927 2310 184 2007 Fragments arranged by size: 2310 2007 1390 927 254 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3029 10 Size: 54 1425 343 1197 4053 Fragments arranged by size: 4053 1425 1197 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3860 3974 4010 5030 5389 5548 518 Size: 648 2694 114 36 1020 359 159 2042 Fragments arranged by size: 2694 2042 1020 648 359 159 114 36 MspA1I CmG'CkG Cuts at: 1606 2101 2125 2140 2233 2855 3103 4041 4120 Size: 495 24 15 93 622 248 938 79 Cuts at: 4120 4488 5165 6313 6558 1606 Size: 368 677 1148 245 2120 Fragments arranged by size: 2120 1148 938 677 622 495 368 248 245 93 79 24 15 NaeI GCC'GGC Cuts at: 2041 2096 2041 Size: 55 7017 NarI GG'CG_CC Cuts at: 2015 2015 Size: 7072 NcoI C'CATG_G Cuts at: 513 1065 2004 2322 3298 513 Size: 552 939 318 976 4287 Fragments arranged by size: 4287 976 939 552 318 NdeI CA'TA_TG Cuts at: 387 387 Size: 7072 NgoMIV G'CCGG_C Cuts at: 2039 2094 2039 Size: 55 7017 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7072 NotI GC'GGCC_GC Cuts at: 4519 4519 Size: 7072 NsiI A_TGCA'T Cuts at: 3129 3769 3129 Size: 640 6432 NspI r_CATG'y Cuts at: 1132 1229 3859 4430 5975 1132 Size: 97 2630 571 1545 2229 Fragments arranged by size: 2630 2229 1545 571 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7072 PciI A'CATG_T Cuts at: 5971 5971 Size: 7072 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7072 PmeI GTTT'AAAC Cuts at: 4376 4376 Size: 7072 PmlI CAC'GTG Cuts at: 4012 4012 Size: 7072 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5141 5173 6654 6686 5141 Size: 32 1481 32 5527 Fragments arranged by size: 5527 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7072 PsiI TTA'TAA Cuts at: 4642 4642 Size: 7072 PstI C_TGCA'G Cuts at: 830 2124 2854 3540 4119 4424 830 Size: 1294 730 686 579 305 3478 Fragments arranged by size: 3478 1294 730 686 579 305 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7072 PvuII CAG'CTG Cuts at: 2125 2140 2233 2855 3103 4041 2125 Size: 15 93 622 248 938 5156 Fragments arranged by size: 5156 938 622 248 93 15 RsrII CG'GwC_CG Cuts at: 4294 4294 Size: 7072 SacII CC_GC'GG Cuts at: 2102 2102 Size: 7072 SalI G'TCGA_C Cuts at: 1936 4414 1936 Size: 2478 4594 SapI GCTCTTCn'nnn_ Cuts at: 2822 2822 Size: 7072 SbfI CC_TGCA'GG Cuts at: 4424 4424 Size: 7072 ScaI AGT'ACT Cuts at: 3398 5320 7032 3398 Size: 1922 1712 3438 Fragments arranged by size: 3438 1922 1712 SexAI A'CCwGG_T Cuts at: 3195 3900 3195 Size: 705 6367 SfcI C'TryA_G Cuts at: 826 1045 2120 2850 3322 3536 4115 4420 5555 Size: 219 1075 730 472 214 579 305 1135 Cuts at: 5555 6236 6427 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1075 730 681 579 472 305 219 214 191 SfoI GGC'GCC Cuts at: 2016 2016 Size: 7072 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7072 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7072 SmaI CCC'GGG Cuts at: 1872 4399 1872 Size: 2527 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 2297 2503 2833 3446 5176 Size: 197 926 297 57 206 330 613 1730 Cuts at: 5176 6077 6339 820 Size: 901 262 1553 Fragments arranged by size: 1730 1553 926 901 613 330 297 262 206 197 57 SnaBI TAC'GTA Cuts at: 493 4863 493 Size: 4370 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7072 SphI G_CATG'C Cuts at: 1132 4430 1132 Size: 3298 3774 SspI AAT'ATT Cuts at: 5 52 4996 5 Size: 47 4944 2081 Fragments arranged by size: 4944 2081 47 StuI AGG'CCT Cuts at: 1517 2443 3657 1517 Size: 926 1214 4932 Fragments arranged by size: 4932 1214 926 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 2493 3298 4511 513 Size: 552 939 318 171 805 1213 3074 Fragments arranged by size: 3074 1213 939 805 552 318 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2639 3903 5099 5116 5269 5454 5899 Size: 1255 1059 1264 1196 17 153 185 445 Cuts at: 5899 325 Size: 1498 Fragments arranged by size: 1498 1264 1255 1196 1059 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2531 3138 3396 Size: 274 80 33 51 1443 554 607 258 Cuts at: 3396 5318 7030 96 Size: 1922 1712 138 Fragments arranged by size: 1922 1712 1443 607 554 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3680 3712 7032 7064 3680 Size: 32 3320 32 3688 Fragments arranged by size: 3688 3320 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4273 1316 Size: 2957 4115 XbaI T'CTAG_A Cuts at: 4408 4408 Size: 7072 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2329 2767 2329 Size: 438 6634 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7072 XmaI C'CCGG_G Cuts at: 1870 4397 1870 Size: 2527 4545 XmnI GAAnn'nnTTC Cuts at: 2612 5201 2612 Size: 2589 4483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4880 276 Size: 53 83 186 936 3346 2468 Fragments arranged by size: 3346 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I DraIII EcoICRI EcoRV FspAI NruI PacI PfoI PpuMI PspOMI PspXI PsrI SacI SanDI SfiI SrfI SwaI