(Linear) (Six-base) MAPSORT of: KIBB5739.seq Check: 6864 from: 1 to: 2367 KIBB5739.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2227 2367 Size: 2227 140 AclI AA'CG_TT Cuts at: 0 1754 2367 Size: 1754 613 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 362 940 1345 1353 1663 1837 2367 Size: 362 578 405 8 310 174 530 Fragments arranged by size: 578 530 405 362 310 174 8 AflIII A'CryG_T Cuts at: 0 1079 2367 Size: 1079 1288 AhdI GACnn_n'nnGTC Cuts at: 0 2295 2367 Size: 2295 72 AlwNI CAG_nnn'CTG Cuts at: 0 465 1279 1416 2016 2219 2237 2367 Size: 465 814 137 600 203 18 130 Fragments arranged by size: 814 600 465 203 137 130 18 ApoI r'AATT_y Cuts at: 0 218 299 425 2367 Size: 218 81 126 1942 Fragments arranged by size: 1942 218 126 81 AvaI C'yCGr_G Cuts at: 0 1583 2367 Size: 1583 784 BanI G'GyrC_C Cuts at: 0 9 1959 2367 Size: 9 1950 408 Fragments arranged by size: 1950 408 9 BanII G_rGCy'C Cuts at: 0 2333 2367 Size: 2333 34 BbeI G_GCGC'C Cuts at: 0 13 2367 Size: 13 2354 BbsI GAAGACnn'nnnn_ Cuts at: 0 610 2367 Size: 610 1757 BbvCI CC'TCA_GC Cuts at: 0 224 2367 Size: 224 2143 BciVI GTATCCnnnnn_n' Cuts at: 0 514 711 762 2367 Size: 514 197 51 1605 Fragments arranged by size: 1605 514 197 51 BfrBI ATG'CAT Cuts at: 0 1122 1762 2367 Size: 1122 640 605 Fragments arranged by size: 1122 640 605 BglI GCCn_nnn'nGGC Cuts at: 0 64 2367 Size: 64 2303 Bme1580I G_kGCm'C Cuts at: 0 943 1964 2367 Size: 943 1021 403 Fragments arranged by size: 1021 943 403 BmgBI CAC'GTC Cuts at: 0 1082 2367 Size: 1082 1285 BmrI ACTGGGnnnn_n' Cuts at: 0 1098 2367 Size: 1098 1269 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2250 2367 Size: 2250 117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 1696 2367 Size: 50 1646 671 Fragments arranged by size: 1646 671 50 Bpu10I CC'TnA_GC Cuts at: 0 224 2367 Size: 224 2143 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 277 519 849 1426 2367 Size: 220 57 242 330 577 941 Fragments arranged by size: 941 577 330 242 220 57 BsaAI yAC'GTr Cuts at: 0 2007 2367 Size: 2007 360 BsaBI GATnn'nnATC Cuts at: 0 448 2367 Size: 448 1919 BsaHI Gr'CG_yC Cuts at: 0 10 1179 2367 Size: 10 1169 1188 Fragments arranged by size: 1188 1169 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2254 2284 2367 Size: 2254 30 83 Fragments arranged by size: 2254 83 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 223 296 1045 1075 2341 2367 Size: 223 73 749 30 1266 26 Fragments arranged by size: 1266 749 223 73 30 26 BseYI C'CCAG_C Cuts at: 0 22 1094 2367 Size: 22 1072 1273 Fragments arranged by size: 1273 1072 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 226 2029 2182 2367 Size: 184 42 1803 153 185 Fragments arranged by size: 1803 185 184 153 42 BsiEI CG_ry'CG Cuts at: 0 39 52 90 94 144 2367 Size: 39 13 38 4 50 2223 Fragments arranged by size: 2223 50 39 38 13 4 BsiHKAI G_wGCw'C Cuts at: 0 1043 1151 1664 1712 2126 2367 Size: 1043 108 513 48 414 241 Fragments arranged by size: 1043 513 414 241 108 48 BsmI GAATG_Cn' Cuts at: 0 878 1269 1762 1936 2367 Size: 878 391 493 174 431 Fragments arranged by size: 878 493 431 391 174 BsmBI CGTCTCn'nnnn_ Cuts at: 0 39 2367 Size: 39 2328 Bsp1286I G_dGCh'C Cuts at: 0 943 1043 1151 1664 1712 1964 2126 2333 Size: 943 100 108 513 48 252 162 207 Cuts at: 2333 2367 Size: 34 Fragments arranged by size: 943 513 252 207 162 108 100 48 34 BspHI T'CATG_A Cuts at: 0 1538 2367 Size: 1538 829 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1524 2108 2177 2204 2227 2367 Size: 1524 584 69 27 23 140 Fragments arranged by size: 1524 584 140 69 27 23 BsrBI CCG'CTC Cuts at: 0 897 2085 2367 Size: 897 1188 282 Fragments arranged by size: 1188 897 282 BsrFI r'CCGG_y Cuts at: 0 12 34 89 139 2291 2367 Size: 12 22 55 50 2152 76 Fragments arranged by size: 2152 76 55 50 22 12 BsrGI T'GTAC_A Cuts at: 0 526 1133 2367 Size: 526 607 1234 Fragments arranged by size: 1234 607 526 BssSI C'ACGA_G Cuts at: 0 1381 2367 Size: 1381 986 BstAPI GCAn_nnn'nTGC Cuts at: 0 2196 2367 Size: 2196 171 BstXI CCAn_nnnn'nTGG Cuts at: 0 1854 2367 Size: 1854 513 Bsu36I CC'TnA_GG Cuts at: 0 1457 2367 Size: 1457 910 BtgI C'CryG_G Cuts at: 0 94 317 1293 2367 Size: 94 223 976 1074 Fragments arranged by size: 1074 976 223 94 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 190 195 225 230 2367 Size: 190 5 30 5 2137 Fragments arranged by size: 2137 190 30 5 5 EaeI y'GGCC_r Cuts at: 0 36 87 91 141 1022 2367 Size: 36 51 4 50 881 1345 Fragments arranged by size: 1345 881 51 50 36 4 EagI C'GGCC_G Cuts at: 0 36 87 91 141 2367 Size: 36 51 4 50 2226 Fragments arranged by size: 2226 51 50 36 4 EarI CTCTTCn'nnn_ Cuts at: 0 314 338 817 962 1501 1987 2367 Size: 314 24 479 145 539 486 380 Fragments arranged by size: 539 486 479 380 314 145 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 92 1975 2367 Size: 92 1883 392 Fragments arranged by size: 1883 392 92 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 362 940 1345 1353 1663 1696 1837 Size: 50 312 578 405 8 310 33 141 Cuts at: 1837 2367 Size: 530 Fragments arranged by size: 578 530 405 312 310 141 50 33 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 2200 2367 Size: 2200 167 EcoO109I rG'GnC_Cy Cuts at: 0 147 838 2367 Size: 147 691 1529 Fragments arranged by size: 1529 691 147 EcoRI G'AATT_C Cuts at: 0 218 2367 Size: 218 2149 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 513 582 627 843 886 1530 2367 Size: 513 69 45 216 43 644 837 Fragments arranged by size: 837 644 513 216 69 45 43 FseI GG_CCGG'CC Cuts at: 0 93 2367 Size: 93 2274 HaeII r_GCGC'y Cuts at: 0 13 2367 Size: 13 2354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 187 219 469 501 1164 1196 1681 1713 Size: 187 32 250 32 663 32 485 32 Cuts at: 1713 1909 1941 2254 2281 2286 2313 2367 Size: 196 32 313 27 5 27 54 Fragments arranged by size: 663 485 313 250 196 187 54 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 0 935 2367 Size: 935 1432 KasI G'GCGC_C Cuts at: 0 9 2367 Size: 9 2358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 690 944 1871 2367 Size: 690 254 927 496 Fragments arranged by size: 927 690 496 254 MscI TGG'CCA Cuts at: 0 1024 2367 Size: 1024 1343 MslI CAynn'nnrTG Cuts at: 0 1855 1969 2005 2367 Size: 1855 114 36 362 Fragments arranged by size: 1855 362 114 36 MspA1I CmG'CkG Cuts at: 0 96 120 135 228 850 1098 2036 2115 Size: 96 24 15 93 622 248 938 79 Cuts at: 2115 2367 Size: 252 Fragments arranged by size: 938 622 252 248 96 93 79 24 15 NaeI GCC'GGC Cuts at: 0 36 91 2367 Size: 36 55 2276 Fragments arranged by size: 2276 55 36 NarI GG'CG_CC Cuts at: 0 10 2367 Size: 10 2357 NcoI C'CATG_G Cuts at: 0 317 1293 2367 Size: 317 976 1074 Fragments arranged by size: 1074 976 317 NgoMIV G'CCGG_C Cuts at: 0 34 89 2367 Size: 34 55 2278 Fragments arranged by size: 2278 55 34 NsiI A_TGCA'T Cuts at: 0 1124 1764 2367 Size: 1124 640 603 Fragments arranged by size: 1124 640 603 NspI r_CATG'y Cuts at: 0 1854 2367 Size: 1854 513 PmlI CAC'GTG Cuts at: 0 2007 2367 Size: 2007 360 PstI C_TGCA'G Cuts at: 0 119 849 1535 2114 2367 Size: 119 730 686 579 253 Fragments arranged by size: 730 686 579 253 119 PvuII CAG'CTG Cuts at: 0 120 135 228 850 1098 2036 2367 Size: 120 15 93 622 248 938 331 Fragments arranged by size: 938 622 331 248 120 93 15 RsrII CG'GwC_CG Cuts at: 0 2289 2367 Size: 2289 78 SacII CC_GC'GG Cuts at: 0 97 2367 Size: 97 2270 SapI GCTCTTCn'nnn_ Cuts at: 0 817 2367 Size: 817 1550 ScaI AGT'ACT Cuts at: 0 1393 2367 Size: 1393 974 SexAI A'CCwGG_T Cuts at: 0 1190 1895 2367 Size: 1190 705 472 Fragments arranged by size: 1190 705 472 SfcI C'TryA_G Cuts at: 0 115 845 1317 1531 2110 2367 Size: 115 730 472 214 579 257 Fragments arranged by size: 730 579 472 257 214 115 SfoI GGC'GCC Cuts at: 0 11 2367 Size: 11 2356 SmlI C'TyrA_G Cuts at: 0 235 292 498 828 1441 2367 Size: 235 57 206 330 613 926 Fragments arranged by size: 926 613 330 235 206 57 StuI AGG'CCT Cuts at: 0 438 1652 2367 Size: 438 1214 715 Fragments arranged by size: 1214 715 438 StyI C'CwwG_G Cuts at: 0 317 488 1293 2367 Size: 317 171 805 1074 Fragments arranged by size: 1074 805 317 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 634 1898 2367 Size: 634 1264 469 Fragments arranged by size: 1264 634 469 TatI w'GTAC_w Cuts at: 0 526 1133 1391 2367 Size: 526 607 258 976 Fragments arranged by size: 976 607 526 258 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1675 1707 2367 Size: 1675 32 660 Fragments arranged by size: 1675 660 32 Tth111I GACn'n_nGTC Cuts at: 0 2268 2367 Size: 2268 99 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 324 762 2367 Size: 324 438 1605 Fragments arranged by size: 1605 438 324 XmnI GAAnn'nnTTC Cuts at: 0 607 2367 Size: 607 1760 Enzymes that do cut: AarI AclI AcuI AflIII AhdI AlwNI ApoI AvaI BanI BanII BbeI BbsI BbvCI BciVI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI Bsu36I BtgI CspCI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PmlI PstI PvuII RsrII SacII SapI ScaI SexAI SfcI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI BclI BglII BlpI BmtI BsaI BsaWI BsiWI BspEI BsrDI BssHII BstBI BstEII BstYI BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI EcoICRI EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI SacI SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI ZraI