################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AvrII BalI BbeI BbrPI BbvCI BciVI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT14I FseI HaeII HaeIII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeI MaeII MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 193 GTATAC GT^[AC][GT]AC AcsI 871 AAATTT [GA]^AATT[CT] AflII 1122 CTTAAG C^TTAAG ApoI 871 AAATTT [GA]^AATT[CT] BamHI 482 GGATCC G^GATCC BanI 562 GGTGCC G^G[CT][GA]CC BclI 572 TGATCA T^GATCA BfrI 1122 CTTAAG C^TTAAG BpmI 1090 CTGGAG CTGGAG(16/14) BsaBI 786 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 1093 GAGGAG GAGGAG(10/8) Bsp1407I 371 TGTACA T^GTACA BspMI 1048 ACCTGC ACCTGC(4/8) BsrGI 371 TGTACA T^GTACA Bst1107I 193 GTATAC GTA^TAC BstZ17I 193 GTATAC GTA^TAC DraI 868 TTTAAA TTT^AAA DsaI 596 CCACGG C^C[GA][CT]GG EcoNI 1089 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 79 GATATC GAT^ATC EcoT22I 924 ATGCAT ATGCA^T FauI 1063 GCGGG CCCGC(4/6) FbaI 572 TGATCA T^GATCA HapII 463 CCGG C^CGG HindIII 1125 AAGCTT A^AGCTT HpaII 463 CCGG C^CGG MamI 786 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 478 CAGCGG C[AC]G^C[GT]G MspI 463 CCGG C^CGG NsiI 924 ATGCAT ATGCA^T NspI 1025 GCATGT [GA]CATG^[CT] PleI 58 GACTC GAGTC(4/5) SapI 972 GAAGAGC GCTCTTC(1/4) SexAI 1102 ACCTGGT A^CC[AT]GGT SfcI 980 CTGTAG C^T[GA][CT]AG SmlI 1122 CTTAAG C^T[CT][GA]AG SspBI 371 TGTACA T^GTACA TfiI 459 GAATC G^A[AT]TC XcmI 729 CCAGGTAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 480 GCGG CCGC(-3/-1) 1063 GCGG AfaI 372 GTAC GT^AC 1035 GTAC AspHI 133 GAGCAC G[AT]GC[AT]^C 310 GAGCAC AvaI 109 CCCGAG C^[CT]CG[GA]G 286 CCCGAG AviII 173 TGCGCA TGC^GCA 350 TGCGCA BanII 742 GGGCTC G[GA]GC[CT]^C 975 GAGCCC BcgI 135 GCACTCGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 312 GCACTCGGTTCG BsgI 475 GTGCAG GTGCAG(16/14) 988 GTGCAG BsiHKAI 133 GAGCAC G[AT]GC[AT]^C 310 GAGCAC BsmFI 39 GGGAC GGGAC(10/14) 952 GTCCC BsoBI 109 CCCGAG C^[CT]CG[GA]G 286 CCCGAG BstF5I 1182 CATCC GGATG(2/0) 501 GGATG BstYI 482 GGATCC [GA]^GATC[CT] 1118 GGATCT CfoI 174 GCGC GCG^C 351 GCGC EarI 610 CTCTTC CTCTTC(1/4) 972 GAAGAG FokI 1182 CATCC GGATG(9/13) 501 GGATG FspI 173 TGCGCA TGC^GCA 350 TGCGCA HhaI 174 GCGC GCG^C 351 GCGC HinP1I 174 GCGC G^CGC 351 GCGC HinfI 58 GACTC G^A[ACGT]TC 459 GAATC HphI 1173 GGTGA GGTGA(8/7) 554 TCACC Ksp632I 610 CTCTTC CTCTTC(1/4) 972 GAAGAG MaeIII 73 GTCAC ^GT[ACGT]AC 1174 GTGAC MflI 482 GGATCC [GA]^GATC[CT] 1118 GGATCT NlaIV 482 GGATCC GG[ACGT]^[ACGT]CC 562 GGTGCC RsaI 372 GTAC GT^AC 1035 GTAC Tsp45I 73 GTCAC ^GT[GC]AC 1174 GTGAC Tsp509I 865 AATT ^AATT 872 AATT XhoII 482 GGATCC [GA]^GATC[CT] 1118 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AlwI BbsI BbvI BmyI BpuAI BsaJI BsiYI BslI Bsp1286I BsrDI BsrI BssKI BstNI Cac8I DdeI DpnII EcoRII Fnu4HI ItaI MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI Tru9I TseI TspRI