################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AhdI 248 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 469 GGATC GGATC(4/5) Asp700I 494 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 248 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 46 GGCACC G^G[CT][GA]CC BbsI 401 GTCTTC GAAGAC(2/6) BbvCI 27 GCTGAGG CCTCAGC(-5/-2) BcnI 43 CCGGG CC^[GC]GG BmyI 45 GGGCAC G[AGT]GC[ACT]^C BpuAI 401 GTCTTC GAAGAC(2/6) BsgI 329 GTGCAG GTGCAG(16/14) BsiYI 178 CCACCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 178 CCACCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 16 GGGAC GGGAC(10/14) BsmI 271 GAATGC GAATGC(1/-1) Bsp1286I 45 GGGCAC G[AGT]GC[ACT]^C BssKI 43 CCGGG ^CC[ACGT]GG BstF5I 277 CATCC GGATG(2/0) CfoI 55 GCGC GCG^C DpnII 470 GATC ^GATC Eam1105I 248 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 634 GAAGAG CTCTTC(1/4) EcoRI 648 GAATTC G^AATTC FokI 277 CATCC GGATG(9/13) HapII 43 CCGG C^CGG HhaI 55 GCGC GCG^C HinP1I 55 GCGC G^CGC HpaII 43 CCGG C^CGG Ksp632I 634 GAAGAG CTCTTC(1/4) MboI 470 GATC ^GATC MseI 220 TTAA T^TAA MspI 43 CCGG C^CGG NciI 43 CCGGG CC^[GC]GG NdeII 470 GATC ^GATC NlaIV 46 GGCACC GG[ACGT]^[ACGT]CC Sau3AI 470 GATC ^GATC ScrFI 43 CCGGG CC^[ACGT]GG SmlI 235 CTTGAG C^T[CT][GA]AG Tru9I 220 TTAA T^TAA TspRI 23 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 494 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 10 GCGG CCGC(-3/-1) 33 GCGG AcsI 167 AAATTC [GA]^AATT[CT] 648 GAATTC AlwNI 302 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG 653 CAGTTTCTG ApoI 167 AAATTC [GA]^AATT[CT] 648 GAATTC BbvI 331 GCAGC GCAGC(8/12) 394 GCTGC BsaJI 50 CCAAGG C^C[ACGT][ACGT]GG 605 CCAAGG DdeI 28 CTGAG C^T[ACGT]AG 575 CTAAG EcoT14I 50 CCAAGG C^C[AT][AT]GG 605 CCAAGG Fnu4HI 331 GCAGC GC^[ACGT]GC 394 GCTGC HinfI 82 GAATC G^A[ACGT]TC 448 GAATC ItaI 331 GCAGC GC^[ACGT]GC 394 GCTGC MboII 634 GAAGA GAAGA(8/7) 402 TCTTC MslI 291 CACTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 554 CATTTTTGTG MwoI 33 GCGGAACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 431 GCAGGGTTAGC NspI 397 GCATGT [GA]CATG^[CT] 504 ACATGC SfcI 362 CTATAG C^T[GA][CT]AG 455 CTGTAG StyI 50 CCAAGG C^C[AT][AT]GG 605 CCAAGG TfiI 82 GAATC G^A[AT]TC 448 GAATC TseI 331 GCAGC G^C[AT]GC 394 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AfaI AluI BfaI HaeIII MaeI MnlI NlaIII RsaI SspI Tsp509I