################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BanII BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HgaI HincII HindII HpaI KpnI Ksp632I KspI MaeII MluI MluNI MroI MscI MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 1155 CGCG CG^CG AcyI 910 GGCGCC G[GA]^CG[CT]C AspI 936 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 850 CCCGAG C^[CT]CG[GA]G BanI 910 GGCGCC G^G[CT][GA]CC BbeI 910 GGCGCC GGCGC^C BbsI 273 GAAGAC GAAGAC(2/6) BclI 534 TGATCA T^GATCA BpmI 1171 CTGGAG CTGGAG(16/14) BpuAI 273 GAAGAC GAAGAC(2/6) BsaBI 469 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 910 GGCGCC G[GA]^CG[CT]C BsaI 941 GGTCTC GGTCTC(1/5) BseRI 251 CTCCTC GAGGAG(10/8) BsmFI 73 GGGAC GGGAC(10/14) BsoBI 850 CCCGAG C^[CT]CG[GA]G BspMI 267 GCAGGT ACCTGC(4/8) BsrBI 1174 GAGCGG CCGCTC(-3/-3) BsrDI 93 CATTGC GCAATG(2/0) BssSI 187 CACGAG CACGAG(-5/-1) BstUI 1155 CGCG CG^CG DraII 73 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 73 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 368 GATATC GAT^ATC EcoT14I 240 CCATGG C^C[AT][AT]GG FauI 146 CCCGC CCCGC(4/6) FbaI 534 TGATCA T^GATCA Hin1I 910 GGCGCC G[GA]^CG[CT]C HindIII 287 AAGCTT A^AGCTT KasI 910 GGCGCC G^GCGCC MaeIII 1151 GTAAC ^GT[ACGT]AC MamI 469 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1089 CAATTG C^AATTG MseI 950 TTAA T^TAA MslI 427 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1089 CAATTG C^AATTG MvnI 1155 CGCG CG^CG NarI 910 GGCGCC GG^CGCC NcoI 240 CCATGG C^CATGG PflFI 936 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 73 GGGACCC [GA]G^G[AT]CC[CT] PstI 505 CTGCAG CTGCA^G SexAI 113 ACCAGGT A^CC[AT]GGT SfoI 910 GGCGCC GGC^GCC SmlI 876 CTTGAG C^T[CT][GA]AG StyI 240 CCATGG C^C[AT][AT]GG TfiI 791 GAATC G^A[AT]TC Tru9I 950 TTAA T^TAA Tth111I 936 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 147 CCGC CCGC(-3/-1) 1176 GCGG AcsI 567 GAATTT [GA]^AATT[CT] 1054 GAATTT Aor51HI 527 AGCGCT AGC^GCT 587 AGCGCT ApoI 567 GAATTT [GA]^AATT[CT] 1054 GAATTT Asp700I 273 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 564 GAAGAATTTC AvaII 48 GGACC G^G[AT]CC 74 GGACC BamHI 35 GGATCC G^GATCC 1011 GGATCC BcnI 165 CCCGG CC^[GC]GG 956 CCCGG BsmAI 226 GAGAC GTCTC(1/5) 942 GTCTC BstF5I 1026 CATCC GGATG(2/0) 786 GGATG Cac8I 82 GCTCGC GC[ACGT]^[ACGT]GC 289 GCTTGC Cfr13I 48 GGACC G^G[ACGT]CC 74 GGACC DsaI 240 CCATGG C^C[GA][CT]GG 782 CCGTGG EaeI 167 CGGCCA [CT]^GGCC[GA] 779 TGGCCG Eco47III 527 AGCGCT AGC^GCT 587 AGCGCT FokI 1026 CATCC GGATG(9/13) 786 GGATG HapII 166 CCGG C^CGG 957 CCGG HpaII 166 CCGG C^CGG 957 CCGG MspI 166 CCGG C^CGG 957 CCGG NciI 165 CCCGG CC^[GC]GG 956 CCCGG PflMI 39 CCATAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 216 CCAGGATTTGG PleI 129 GAGTC GAGTC(4/5) 657 GAGTC Sau96I 48 GGACC G^G[ACGT]CC 74 GGACC SfcI 505 CTGCAG C^T[GA][CT]AG 1115 CTACAG Van91I 39 CCATAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 216 CCAGGATTTGG XmnI 273 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 564 GAAGAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AluI AlwI BbvI BfaI BsaJI BsiYI BslI BsrI BssKI BstNI BstYI CfoI DdeI DpnII EcoRII Fnu4HI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI MaeI MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI TseI Tsp509I TspRI XhoII