(Circular) (Six-base) MAPSORT of: pFN21AE1876.seq Check: 4197 from: 1 to: 5899 pFN21AE1876 5899 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2263 2263 Size: 5899 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3242 1937 Size: 1305 4594 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2588 3962 5346 5614 1632 Size: 956 1374 1384 268 1917 Fragments arranged by size: 1917 1384 1374 956 268 AfeI AGC'GCT Cuts at: 2534 2594 2534 Size: 60 5839 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 4798 5775 1203 Size: 3595 977 1327 Fragments arranged by size: 3595 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 3642 5214 3642 Size: 1572 4327 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 2572 3059 3208 3434 3586 5695 1493 Size: 1079 487 149 226 152 2109 1697 Fragments arranged by size: 2109 1697 1079 487 226 152 149 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 2855 3224 1870 Size: 73 912 369 4545 Fragments arranged by size: 4545 912 369 73 BamHI G'GATC_C Cuts at: 2040 3016 3229 3596 2040 Size: 976 213 367 4343 Fragments arranged by size: 4343 976 367 213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2915 3220 4675 618 Size: 325 217 627 1128 305 1455 1842 Fragments arranged by size: 1842 1455 1128 627 325 305 217 BanII G_rGCy'C Cuts at: 1391 3218 1391 Size: 1827 4072 BbeI G_GCGC'C Cuts at: 2919 2919 Size: 5899 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2285 928 Size: 971 386 4542 Fragments arranged by size: 4542 971 386 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 2539 1548 Size: 991 4908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4510 136 Size: 107 122 71 631 605 2838 1525 Fragments arranged by size: 2838 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 3316 3316 Size: 5899 Bme1580I G_kGCm'C Cuts at: 1790 3960 5116 1790 Size: 2170 1156 2573 Fragments arranged by size: 2573 2170 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5899 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2952 3630 4588 Size: 416 53 83 399 24 1535 678 958 Cuts at: 4588 442 Size: 1753 Fragments arranged by size: 1753 1535 958 678 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3197 4559 1239 Size: 1958 1362 2579 Fragments arranged by size: 2579 1958 1362 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2902 4024 4889 5187 2902 Size: 1122 865 298 3614 Fragments arranged by size: 3614 1122 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2952 4562 882 Size: 617 1453 1610 2219 Fragments arranged by size: 2219 1610 1453 617 BsaAI yAC'GTr Cuts at: 493 1204 3690 493 Size: 711 2486 2702 Fragments arranged by size: 2702 2486 711 BsaBI GATnn'nnATC Cuts at: 2478 5893 2478 Size: 3415 2484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2916 3706 4088 4723 Size: 53 83 186 936 1383 790 382 635 Cuts at: 4723 275 Size: 1451 Fragments arranged by size: 1451 1383 936 790 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4332 4730 5004 5151 5562 4332 Size: 398 274 147 411 4669 Fragments arranged by size: 4669 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 2664 2694 1178 Size: 30 165 30 795 30 436 30 4383 Fragments arranged by size: 4383 795 436 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2247 1186 Size: 1061 4838 BseYI C'CCAG_C Cuts at: 2989 5102 5598 2989 Size: 2113 496 3290 Fragments arranged by size: 3290 2113 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5326 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3349 4110 5138 1540 Size: 275 186 1348 761 1028 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3960 4045 5116 3960 Size: 85 1071 4743 Fragments arranged by size: 4743 1071 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3218 3960 4045 5116 1391 Size: 399 1428 742 85 1071 2174 Fragments arranged by size: 2174 1428 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5562 5562 Size: 5899 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2263 3254 844 Size: 1419 991 3489 Fragments arranged by size: 3489 1419 991 BsrBI CCG'CTC Cuts at: 1394 1823 3181 3786 1394 Size: 429 1358 605 3507 Fragments arranged by size: 3507 1358 605 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2097 4394 4568 65 Size: 1183 849 2297 174 1396 Fragments arranged by size: 2297 1396 1183 849 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4543 4730 1700 Size: 110 2733 187 2869 Fragments arranged by size: 2869 2733 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5899 BssSI C'ACGA_G Cuts at: 2192 3959 4971 2192 Size: 1767 1012 3120 Fragments arranged by size: 3120 1767 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5899 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstYI r'GATC_y Cuts at: 1972 2040 2503 3016 3229 3596 3981 3998 5450 Size: 68 463 513 213 367 385 17 1452 Cuts at: 5450 5536 5548 5894 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 513 463 385 367 346 213 86 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 2245 2787 513 Size: 552 1180 542 3625 Fragments arranged by size: 3625 1180 552 542 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5160 Fragments arranged by size: 5160 683 56 BtsI GCAGTG_nn' Cuts at: 3415 4209 4229 3415 Size: 794 20 5085 Fragments arranged by size: 5085 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2784 3346 Size: 54 1425 50 275 18 342 612 562 Cuts at: 3346 4235 5864 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 612 562 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3346 1537 Size: 275 1534 4090 Fragments arranged by size: 4090 1534 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3829 1353 Size: 2476 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4486 4860 5006 387 Size: 1064 138 2897 374 146 1280 Fragments arranged by size: 2897 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2588 3197 3962 4559 5346 5614 1239 Size: 393 956 609 765 597 787 268 1524 Fragments arranged by size: 1524 956 787 765 609 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2079 2079 Size: 5899 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 EcoRV GAT'ATC Cuts at: 2375 2375 Size: 5899 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 2536 2596 2919 5046 1678 Size: 858 60 323 2127 2531 Fragments arranged by size: 2531 2127 858 323 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2071 2103 2932 2964 4540 Size: 32 135 32 499 32 829 32 1576 Cuts at: 4540 4572 4614 4646 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1576 829 499 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3243 3489 669 Size: 988 281 1305 246 3079 Fragments arranged by size: 3079 1305 988 281 246 HindIII A'AGCT_T Cuts at: 748 2292 748 Size: 1544 4355 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KasI G'GCGC_C Cuts at: 2915 2915 Size: 5899 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 3094 3498 3094 Size: 404 5495 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2031 2704 3066 5013 5197 1305 Size: 726 673 362 1947 184 2007 Fragments arranged by size: 2007 1947 726 673 362 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4077 Fragments arranged by size: 4077 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2436 3857 4216 4375 518 Size: 648 1270 1421 359 159 2042 Fragments arranged by size: 2042 1421 1270 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3315 3992 5140 5385 1606 Size: 1709 677 1148 245 2120 Fragments arranged by size: 2120 1709 1148 677 245 NarI GG'CG_CC Cuts at: 2916 2916 Size: 5899 NcoI C'CATG_G Cuts at: 513 1065 2245 513 Size: 552 1180 4167 Fragments arranged by size: 4167 1180 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 3257 4802 1132 Size: 97 2028 1545 2229 Fragments arranged by size: 2229 2028 1545 97 PasI CC'CwG_GG Cuts at: 1348 2057 1348 Size: 709 5190 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 2050 2227 1108 Size: 942 177 4780 Fragments arranged by size: 4780 942 177 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2740 2772 3131 3163 3968 4000 5481 5513 2740 Size: 32 359 32 805 32 1481 32 3126 Fragments arranged by size: 3126 1481 805 359 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2079 2079 Size: 5899 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PstI C_TGCA'G Cuts at: 830 2514 3251 830 Size: 1684 737 3478 Fragments arranged by size: 3478 1684 737 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SanDI GG'GwC_CC Cuts at: 2079 2079 Size: 5899 SbfI CC_TGCA'GG Cuts at: 3251 3251 Size: 5899 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SexAI A'CCwGG_T Cuts at: 2118 2118 Size: 5899 SfcI C'TryA_G Cuts at: 826 1045 2510 3120 3247 4382 5063 5254 826 Size: 219 1465 610 127 1135 681 191 1471 Fragments arranged by size: 1471 1465 1135 681 610 219 191 127 SfoI GGC'GCC Cuts at: 2917 2917 Size: 5899 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 3226 1872 Size: 1354 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2881 4003 4904 5166 820 Size: 197 926 938 1122 901 262 1553 Fragments arranged by size: 1553 1122 938 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5899 StyI C'CwwG_G Cuts at: 513 1065 2245 3338 513 Size: 552 1180 1093 3074 Fragments arranged by size: 3074 1180 1093 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2476 3926 3943 4096 4281 4726 325 Size: 1255 896 1450 17 153 185 445 1498 Fragments arranged by size: 1498 1450 1255 896 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4145 5857 96 Size: 274 80 33 51 1443 2168 1712 138 Fragments arranged by size: 2168 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5859 5891 5859 Size: 32 5867 Tth111I GACn'n_nGTC Cuts at: 1316 2944 1316 Size: 1628 4271 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 3224 1870 Size: 1354 4545 XmnI GAAnn'nnTTC Cuts at: 2282 2573 4028 2282 Size: 291 1455 4153 Fragments arranged by size: 4153 1455 291 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SrfI SwaI XcmI