(Circular) (Six-base) MAPSORT of: pFN21AE0356.seq Check: 6406 from: 1 to: 6220 pFN21AE0356 6220 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 2933 4347 4720 1563 Size: 1370 1414 373 3063 Fragments arranged by size: 3063 1414 1370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2006 2260 2575 2925 2943 4283 5667 5935 Size: 374 254 315 350 18 1340 1384 268 Cuts at: 5935 1632 Size: 1917 Fragments arranged by size: 1917 1384 1340 374 350 315 268 254 18 AflII C'TTAA_G Cuts at: 820 1017 2807 820 Size: 197 1790 4233 Fragments arranged by size: 4233 1790 197 AflIII A'CryG_T Cuts at: 1203 2548 5119 6096 1203 Size: 1345 2571 977 1327 Fragments arranged by size: 2571 1345 1327 977 AgeI A'CCGG_T Cuts at: 3136 5051 3136 Size: 1915 4305 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2090 3390 1184 Size: 906 1300 4014 Fragments arranged by size: 4014 1300 906 AlwNI CAG_nnn'CTG Cuts at: 3963 5535 3963 Size: 1572 4648 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2717 2747 3301 3529 3755 3907 6016 1493 Size: 1224 30 554 228 226 152 2109 1697 Fragments arranged by size: 2109 1697 1224 554 228 226 152 30 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 3545 1870 Size: 73 1602 4545 Fragments arranged by size: 4545 1602 73 AvrII C'CTAG_G Cuts at: 3046 3046 Size: 6220 BamHI G'GATC_C Cuts at: 2979 3550 3917 2979 Size: 571 367 5282 Fragments arranged by size: 5282 571 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3541 4996 618 Size: 325 217 627 1754 1455 1842 Fragments arranged by size: 1842 1754 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2131 2541 3235 3539 1391 Size: 622 118 410 694 304 4072 Fragments arranged by size: 4072 694 622 410 304 118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3051 3267 928 Size: 971 1152 216 3881 Fragments arranged by size: 3881 1152 971 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4411 4445 1903 Size: 34 2474 34 3678 Fragments arranged by size: 3678 2474 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 2121 1548 Size: 573 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2017 2601 4831 Size: 107 122 71 631 605 345 584 2230 Cuts at: 4831 136 Size: 1525 Fragments arranged by size: 2230 1525 631 605 584 345 122 107 71 BglII A'GATC_T Cuts at: 2911 6215 2911 Size: 3304 2916 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 4281 5437 1790 Size: 2491 1156 2573 Fragments arranged by size: 2573 2491 1156 BmgBI CAC'GTC Cuts at: 1318 2531 1318 Size: 1213 5007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2183 3951 4909 Size: 416 53 83 399 24 766 1768 958 Cuts at: 4909 442 Size: 1753 Fragments arranged by size: 1768 1753 958 766 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2555 2900 3277 3417 4880 1239 Size: 1316 345 377 140 1463 2579 Fragments arranged by size: 2579 1463 1316 377 345 140 Bpu10I CC'TnA_GC Cuts at: 2657 3006 3080 2657 Size: 349 74 5797 Fragments arranged by size: 5797 349 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2712 3304 3427 3461 4345 5210 5508 2712 Size: 592 123 34 884 865 298 3424 Fragments arranged by size: 3424 884 865 592 298 123 34 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 3107 6214 3107 Size: 3107 3113 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4027 4409 5044 275 Size: 53 83 186 936 2494 382 635 1451 Fragments arranged by size: 2494 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2793 3136 4653 5051 5325 5472 5883 2793 Size: 343 1517 398 274 147 411 3130 Fragments arranged by size: 3130 1517 411 398 343 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5995 Fragments arranged by size: 5995 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2122 2125 1186 Size: 865 71 3 5281 Fragments arranged by size: 5281 865 71 3 BseYI C'CCAG_C Cuts at: 2847 3453 5423 5919 2847 Size: 606 1970 496 3148 Fragments arranged by size: 3148 1970 606 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 2130 1163 Size: 573 381 13 5253 Fragments arranged by size: 5253 573 381 13 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2541 4281 4366 5437 2541 Size: 1740 85 1071 3324 Fragments arranged by size: 3324 1740 1071 85 BsmI GAATG_Cn' Cuts at: 2445 3122 3731 3824 2445 Size: 677 609 93 4841 Fragments arranged by size: 4841 677 609 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2131 2541 3235 3539 4281 4366 Size: 399 223 118 410 694 304 742 85 Cuts at: 4366 5437 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 694 410 399 304 223 118 85 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 2577 4109 5839 2577 Size: 1532 1730 2958 Fragments arranged by size: 2958 1730 1532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3208 3575 844 Size: 2364 367 3489 Fragments arranged by size: 3489 2364 367 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2514 4715 4889 65 Size: 1183 1266 2201 174 1396 Fragments arranged by size: 2201 1396 1266 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3136 4864 5051 1700 Size: 110 1326 1728 187 2869 Fragments arranged by size: 2869 1728 1326 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 2153 794 Size: 1359 4861 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstYI r'GATC_y Cuts at: 1972 2323 2911 2979 3550 3917 4302 4319 5771 Size: 351 588 68 571 367 385 17 1452 Cuts at: 5771 5857 5869 6215 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 588 571 385 367 351 346 86 68 17 12 Bsu36I CC'TnA_GG Cuts at: 3267 3267 Size: 6220 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4729 Fragments arranged by size: 4729 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2652 3405 3736 4530 4550 2652 Size: 753 331 794 20 4322 Fragments arranged by size: 4322 794 753 331 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DraIII CAC_nnn'GTG Cuts at: 3105 3105 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3269 5227 809 Size: 507 1953 1958 1802 Fragments arranged by size: 1958 1953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2170 3667 4556 Size: 54 1425 50 275 18 340 1497 889 Cuts at: 4556 6185 8 Size: 1629 43 Fragments arranged by size: 1629 1497 1425 889 340 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3240 4150 1353 Size: 1887 910 3423 Fragments arranged by size: 3423 1887 910 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3113 4807 5181 5327 387 Size: 1064 138 1524 1694 374 146 1280 Fragments arranged by size: 1694 1524 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2539 2539 Size: 6220 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2006 2260 2555 2575 2900 2925 2943 Size: 393 374 254 295 20 325 25 18 Cuts at: 2943 3277 3417 4283 4880 5667 5935 1239 Size: 334 140 866 597 787 268 1524 Fragments arranged by size: 1524 866 787 597 393 374 334 325 295 268 254 140 25 20 18 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 6220 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2848 5367 1678 Size: 1170 2519 2531 Fragments arranged by size: 2531 2519 1170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 2945 2977 3018 Size: 32 135 32 534 32 807 32 41 Cuts at: 3018 3050 3176 3208 4861 4893 4935 4967 1373 Size: 32 126 32 1653 32 42 32 2626 Fragments arranged by size: 2626 1653 807 534 135 126 42 41 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2236 2595 2694 5334 5518 1305 Size: 931 359 99 2640 184 2007 Fragments arranged by size: 2640 2007 931 359 184 99 MscI TGG'CCA Cuts at: 10 64 1489 1832 2172 10 Size: 54 1425 343 340 4058 Fragments arranged by size: 4058 1425 343 340 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 2468 3101 3321 4178 4537 4696 Size: 648 1299 3 633 220 857 359 159 Cuts at: 4696 518 Size: 2042 Fragments arranged by size: 2042 1299 857 648 633 359 220 159 3 MspA1I CmG'CkG Cuts at: 1606 3344 3636 4313 5461 5706 1606 Size: 1738 292 677 1148 245 2120 Fragments arranged by size: 2120 1738 1148 677 292 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4729 Fragments arranged by size: 4729 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2071 2552 3578 5123 1132 Size: 97 842 481 1026 1545 2229 Fragments arranged by size: 2229 1545 1026 842 481 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 2548 5119 2548 Size: 2571 3649 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6220 PfoI T'CCnGG_A Cuts at: 2787 2975 2787 Size: 188 6032 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PstI C_TGCA'G Cuts at: 830 3205 3572 830 Size: 2375 367 3478 Fragments arranged by size: 3478 2375 367 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 3344 3344 Size: 6220 SacI G_AGCT'C Cuts at: 2541 2541 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SapI GCTCTTCn'nnn_ Cuts at: 3240 3240 Size: 6220 SbfI CC_TGCA'GG Cuts at: 3205 3572 3205 Size: 367 5853 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SexAI A'CCwGG_T Cuts at: 2706 2706 Size: 6220 SfcI C'TryA_G Cuts at: 826 1045 3201 3568 4703 5384 5575 826 Size: 219 2156 367 1135 681 191 1471 Fragments arranged by size: 2156 1471 1135 681 367 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2727 2807 3283 3442 3476 4324 Size: 197 926 784 80 476 159 34 848 Cuts at: 4324 5225 5487 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 848 784 476 262 197 159 80 34 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 2457 3226 4144 5 Size: 47 2405 769 918 2081 Fragments arranged by size: 2405 2081 918 769 47 StuI AGG'CCT Cuts at: 1517 3448 1517 Size: 1931 4289 StyI C'CwwG_G Cuts at: 513 1065 2004 3046 3659 513 Size: 552 939 1042 613 3074 Fragments arranged by size: 3074 1042 939 613 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4247 4264 4417 4602 5047 325 Size: 1255 2667 17 153 185 445 1498 Fragments arranged by size: 2667 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3198 3230 3483 3515 6180 6212 3198 Size: 32 253 32 2665 32 3206 Fragments arranged by size: 3206 2665 253 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2165 2165 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 2456 2933 4349 2456 Size: 477 1416 4327 Fragments arranged by size: 4327 1416 477 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI