################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspEI BsrGI BssHII Bst1107I BstBI BstZ17I CpoI DraI DraIII Eam1105I Eco47III EcoNI EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KasI MaeI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI SfoI SfuI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 242 GGTACC G^GTACC AcsI 371 AAATTC [GA]^AATT[CT] AcyI 267 GACGCC G[GA]^CG[CT]C Alw44I 1168 GTGCAC G^TGCAC ApaLI 1168 GTGCAC G^TGCAC ApoI 371 AAATTC [GA]^AATT[CT] Asp718I 242 GGTACC G^GTACC AspHI 1168 GTGCAC G[AT]GC[AT]^C BanI 242 GGTACC G^G[CT][GA]CC BbvCI 69 CCTCAGC CCTCAGC(-5/-2) BcgI 1745 GCAAGAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 267 GACGCC G[GA]^CG[CT]C BsaWI 1826 ACCGGA [AT]^CCGG[AT] BseRI 1501 GAGGAG GAGGAG(10/8) BsiEI 1457 CGGCCG CG[GA][CT]^CG BsiHKAI 1168 GTGCAC G[AT]GC[AT]^C BspHI 668 TCATGA T^CATGA BspLU11I 587 ACATGT A^CATGT BspMI 107 ACCTGC ACCTGC(4/8) BssSI 76 CTCGTG CACGAG(-5/-1) BstAPI 1302 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 191 AGATCC [GA]^GATC[CT] DrdI 1075 GACTTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1457 CGGCCG C^GGCCG EarI 1696 GAAGAG CTCTTC(1/4) EclXI 1457 CGGCCG C^GGCCG Eco52I 1457 CGGCCG C^GGCCG EcoRV 499 GATATC GAT^ATC HaeII 740 GGCGCT [GA]GCGC^[CT] Hin1I 267 GACGCC G[GA]^CG[CT]C HincII 1660 GTCAAC GT[CT]^[GA]AC HindII 1660 GTCAAC GT[CT]^[GA]AC KpnI 242 GGTACC GGTAC^C Ksp632I 1696 GAAGAG CTCTTC(1/4) KspI 1655 CCGCGG CCGC^GG MflI 191 AGATCC [GA]^GATC[CT] NaeI 46 GCCGGC GCC^GGC NcoI 1730 CCATGG C^CATGG NgoMIV 46 GCCGGC G^CCGGC PaeR7I 1753 CTCGAG C^TCGAG RcaI 668 TCATGA T^CATGA SacII 1655 CCGCGG CCGC^GG SapI 1696 GAAGAGC GCTCTTC(1/4) ScaI 221 AGTACT AGT^ACT SexAI 1490 ACCAGGT A^CC[AT]GGT SfiI 1359 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 312 CACCGGCG C[GA]^CCGG[CT]G SphI 1550 GCATGC GCATG^C XcmI 705 CCAGTTCCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1753 CTCGAG C^TCGAG XhoII 191 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 1468 GTAGAC GT^[AC][GT]AC 1537 GTCTAC AlwNI 706 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 910 CAGTTCCTG AocI 333 CCTGAGG CC^T[ACGT]AGG 1568 CCTCAGG BanII 81 GGGCTC G[GA]GC[CT]^C 1423 GGGCTC BbrPI 1198 CACGTG CAC^GTG 1297 CACGTG BglI 74 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1360 GCCATCAAGGC BlpI 810 GCTCAGC GC^T[ACGT]AGC 1777 GCTGAGC BpmI 907 CTCCAG CTGGAG(16/14) 799 CTGGAG Bpu1102I 810 GCTCAGC GC^T[ACGT]AGC 1777 GCTGAGC BsrFI 46 GCCGGC [GA]^CCGG[CT] 313 ACCGGC BstEII 15 GGTCACC G^GT[ACGT]ACC 771 GGTGACC BstPI 15 GGTCACC G^GT[ACGT]ACC 771 GGTGACC BstXI 548 CCAAAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 776 CCACGGAGATGG Bsu36I 333 CCTGAGG CC^T[ACGT]AGG 1568 CCTCAGG CelII 810 GCTCAGC GC^T[ACGT]AGC 1777 GCTGAGC Cfr10I 46 GCCGGC [GA]^CCGG[CT] 313 ACCGGC DraII 1325 AGGACCT [GA]G^G[ACGT]CC[CT] 1505 AGGACCC Eco81I 333 CCTGAGG CC^T[ACGT]AGG 1568 CCTCAGG EcoO109I 1325 AGGACCT [GA]G^G[ACGT]CC[CT] 1505 AGGACCC EcoO65I 15 GGTCACC G^GT[ACGT]ACC 771 GGTGACC EcoT14I 1226 CCAAGG C^C[AT][AT]GG 1730 CCATGG HgaI 267 GACGC GACGC(5/10) 344 GACGC PmaCI 1198 CACGTG CAC^GTG 1297 CACGTG PmlI 1198 CACGTG CAC^GTG 1297 CACGTG PpuMI 1325 AGGACCT [GA]G^G[AT]CC[CT] 1505 AGGACCC PstI 93 CTGCAG CTGCA^G 1516 CTGCAG SfcI 93 CTGCAG C^T[GA][CT]AG 1516 CTGCAG SmaI 993 CCCGGG CCC^GGG 1370 CCCGGG StyI 1226 CCAAGG C^C[AT][AT]GG 1730 CCATGG Tsp509I 372 AATT ^AATT 541 AATT XmaCI 993 CCCGGG C^CCGGG 1370 CCCGGG XmaI 993 CCCGGG C^CCGGG 1370 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI Asp700I AvaI AvaII BalI BbsI BbvI BcnI BmyI BpuAI BsaAI BsaJI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspDI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I ClaI DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XmnI