(Circular) (Six-base) MAPSORT of: pFN21AE2051.seq Check: 2789 from: 1 to: 6556 pFN21AE2051 6556 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2121 2121 Size: 6556 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4366 278 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3474 3543 3899 1937 Size: 1537 69 356 4594 Fragments arranged by size: 4594 1537 356 69 Acc65I G'GTAC_C Cuts at: 2247 3877 2247 Size: 1630 4926 AclI AA'CG_TT Cuts at: 1563 4683 5056 1563 Size: 3120 373 3063 Fragments arranged by size: 3120 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2288 4619 6003 6271 1632 Size: 641 15 2331 1384 268 1917 Fragments arranged by size: 2331 1917 1384 641 268 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6359 AflIII A'CryG_T Cuts at: 1203 2592 3202 3546 5455 6432 1203 Size: 1389 610 344 1909 977 1327 Fragments arranged by size: 1909 1389 1327 977 610 344 AgeI A'CCGG_T Cuts at: 5387 5387 Size: 6556 AhdI GACnn_n'nnGTC Cuts at: 5285 5285 Size: 6556 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6556 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2774 1184 Size: 1590 4966 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2555 2587 2555 Size: 32 6524 AlwNI CAG_nnn'CTG Cuts at: 2716 2920 4299 5871 2716 Size: 204 1379 1572 3401 Fragments arranged by size: 3401 1572 1379 204 ApaLI G'TGCA_C Cuts at: 3173 4613 5769 3173 Size: 1440 1156 3960 Fragments arranged by size: 3960 1440 1156 ApoI r'AATT_y Cuts at: 1493 2376 3865 4091 4243 6352 1493 Size: 883 1489 226 152 2109 1697 Fragments arranged by size: 2109 1697 1489 883 226 152 AseI AT'TA_AT Cuts at: 160 1032 5110 160 Size: 872 4078 1606 Fragments arranged by size: 4078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 AvaI C'yCGr_G Cuts at: 1870 1943 2364 2762 2998 3359 3375 3758 3881 Size: 73 421 398 236 361 16 383 123 Cuts at: 3881 1870 Size: 4545 Fragments arranged by size: 4545 421 398 383 361 236 123 73 16 BamHI G'GATC_C Cuts at: 3886 4253 3886 Size: 367 6189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2247 3877 5332 618 Size: 325 217 627 460 1630 1455 1842 Fragments arranged by size: 1842 1630 1455 627 460 325 217 BanII G_rGCy'C Cuts at: 1391 2090 3432 3875 1391 Size: 699 1342 443 4072 Fragments arranged by size: 4072 1342 699 443 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2276 2595 2697 3402 928 Size: 971 377 319 102 705 4082 Fragments arranged by size: 4082 971 705 377 319 102 BbvCI CC'TCA_GC Cuts at: 2075 2075 Size: 6556 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3739 3773 4747 4781 1903 Size: 34 50 34 1718 34 974 34 3678 Fragments arranged by size: 3678 1718 974 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4448 5658 4448 Size: 1210 5346 BclI T'GATC_A Cuts at: 1548 1548 Size: 6556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2085 3371 5167 Size: 107 122 71 631 605 413 1286 1796 Cuts at: 5167 136 Size: 1525 Fragments arranged by size: 1796 1525 1286 631 605 413 122 107 71 BglII A'GATC_T Cuts at: 6551 6551 Size: 6556 BlpI GC'TnA_GC Cuts at: 2816 3783 3973 2816 Size: 967 190 5399 Fragments arranged by size: 5399 967 190 Bme1580I G_kGCm'C Cuts at: 1790 3177 3303 4617 5773 1790 Size: 1387 126 1314 1156 2573 Fragments arranged by size: 2573 1387 1314 1156 126 BmgBI CAC'GTC Cuts at: 1318 2170 3088 1318 Size: 852 918 4786 Fragments arranged by size: 4786 918 852 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2198 4287 5245 Size: 416 53 83 399 24 781 2089 958 Cuts at: 5245 442 Size: 1753 Fragments arranged by size: 2089 1753 958 781 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6556 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2825 2897 5216 1239 Size: 1586 72 2319 2579 Fragments arranged by size: 2579 2319 1586 72 Bpu10I CC'TnA_GC Cuts at: 2075 2075 Size: 6556 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2372 2485 4681 5546 5844 2372 Size: 113 2196 865 298 3084 Fragments arranged by size: 3084 2196 865 298 113 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5219 882 Size: 617 3720 2219 Fragments arranged by size: 3720 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3205 3304 3547 4347 493 Size: 711 2001 99 243 800 2702 Fragments arranged by size: 2702 2001 800 711 243 99 BsaBI GATnn'nnATC Cuts at: 6550 6550 Size: 6556 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2273 4363 4745 5380 Size: 53 83 186 936 740 2090 382 635 Cuts at: 5380 275 Size: 1451 Fragments arranged by size: 2090 1451 936 740 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3831 4989 5387 5661 5808 6219 3831 Size: 1158 398 274 147 411 4168 Fragments arranged by size: 4168 1158 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 3596 3626 1178 Size: 30 165 30 1152 30 1011 30 4108 Fragments arranged by size: 4108 1152 1011 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3521 1186 Size: 2335 4221 BseYI C'CCAG_C Cuts at: 3651 5759 6255 3651 Size: 2108 496 3952 Fragments arranged by size: 3952 2108 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2734 2818 2890 3326 3335 1163 Size: 573 998 84 72 436 9 4384 Fragments arranged by size: 4384 998 573 436 84 72 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3465 4006 4767 5795 1540 Size: 275 186 1464 541 761 1028 2301 Fragments arranged by size: 2301 1464 1028 761 541 275 186 BsiHKAI G_wGCw'C Cuts at: 3177 4617 4702 5773 3177 Size: 1440 85 1071 3960 Fragments arranged by size: 3960 1440 1071 85 BsmI GAATG_Cn' Cuts at: 4067 4160 4067 Size: 93 6463 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 3177 3303 3432 3875 4617 4702 Size: 399 300 1087 126 129 443 742 85 Cuts at: 4702 5773 1391 Size: 1071 2174 Fragments arranged by size: 2174 1087 1071 742 443 399 300 129 126 85 BspEI T'CCGG_A Cuts at: 6219 6219 Size: 6556 BspHI T'CATG_A Cuts at: 2673 4445 6175 2673 Size: 1772 1730 3054 Fragments arranged by size: 3054 1772 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 3911 844 Size: 1277 1790 3489 Fragments arranged by size: 3489 1790 1277 BsrBI CCG'CTC Cuts at: 1394 1823 2668 2809 2927 4443 1394 Size: 429 845 141 118 1516 3507 Fragments arranged by size: 3507 1516 845 429 141 118 BsrDI GCAATG_nn' Cuts at: 65 1248 2973 3144 3156 3216 5051 5225 65 Size: 1183 1725 171 12 60 1835 174 1396 Fragments arranged by size: 1835 1725 1396 1183 174 171 60 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 2318 5200 5387 1700 Size: 110 241 267 2882 187 2869 Fragments arranged by size: 2882 2869 267 241 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6556 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6556 BssSI C'ACGA_G Cuts at: 2081 4616 5628 2081 Size: 2535 1012 3009 Fragments arranged by size: 3009 2535 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3313 794 Size: 2519 4037 BstBI TT'CG_AA Cuts at: 5360 5360 Size: 6556 BstEII G'GTnAC_C Cuts at: 1184 2020 2776 1184 Size: 836 756 4964 Fragments arranged by size: 4964 836 756 BstXI CCAn_nnnn'nTGG Cuts at: 2560 2788 2560 Size: 228 6328 BstYI r'GATC_y Cuts at: 1972 2196 3886 4253 4638 4655 6107 6193 6205 Size: 224 1690 367 385 17 1452 86 12 Cuts at: 6205 6551 1972 Size: 346 1977 Fragments arranged by size: 1977 1690 1452 385 367 346 224 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2339 3574 2339 Size: 1235 5321 BtgI C'CryG_G Cuts at: 513 1065 2025 2278 2781 3627 3660 3735 513 Size: 552 960 253 503 846 33 75 3334 Fragments arranged by size: 3334 960 846 552 503 253 75 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2337 2573 487 Size: 683 56 1111 236 4470 Fragments arranged by size: 4470 1111 683 236 56 BtsI GCAGTG_nn' Cuts at: 4072 4866 4886 4072 Size: 794 20 5742 Fragments arranged by size: 5742 794 20 ClaI AT'CG_AT Cuts at: 1554 2434 2472 2883 1554 Size: 880 38 411 5227 Fragments arranged by size: 5227 880 411 38 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6521 DraI TTT'AAA Cuts at: 3860 3860 Size: 6556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3086 5563 809 Size: 507 1770 2477 1802 Fragments arranged by size: 2477 1802 1770 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2922 2955 Size: 54 1425 50 275 18 198 894 33 Cuts at: 2955 3054 3462 3673 3849 4003 4892 6521 8 Size: 99 408 211 176 154 889 1629 43 Fragments arranged by size: 1629 1425 894 889 408 275 211 198 176 154 99 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3462 4003 1537 Size: 275 1650 541 4090 Fragments arranged by size: 4090 1650 541 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3696 4486 1353 Size: 2343 790 3423 Fragments arranged by size: 3423 2343 790 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3637 5143 5517 5663 387 Size: 1064 138 2048 1506 374 146 1280 Fragments arranged by size: 2048 1506 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2288 2825 2897 4619 5216 6003 Size: 393 641 15 537 72 1722 597 787 Cuts at: 6003 6271 1239 Size: 268 1524 Fragments arranged by size: 1722 1524 787 641 597 537 393 268 72 15 EcoO109I rG'GnC_Cy Cuts at: 3331 3511 3331 Size: 180 6376 EcoRI G'AATT_C Cuts at: 3865 4243 3865 Size: 378 6178 EcoRV GAT'ATC Cuts at: 2506 2506 Size: 6556 FspI TGC'GCA Cuts at: 5062 5062 Size: 6556 HaeII r_GCGC'y Cuts at: 1678 2749 5703 1678 Size: 1071 2954 2531 Fragments arranged by size: 2954 2531 1071 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3596 3628 5197 5229 5271 Size: 32 135 32 2024 32 1569 32 42 Cuts at: 5271 5303 1373 Size: 32 2626 Fragments arranged by size: 2626 2024 1569 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3667 3900 4146 669 Size: 988 281 1729 233 246 3079 Fragments arranged by size: 3079 1729 988 281 246 233 HindIII A'AGCT_T Cuts at: 748 748 Size: 6556 HpaI GTT'AAC Cuts at: 4146 4146 Size: 6556 KpnI G_GTAC'C Cuts at: 2251 3881 2251 Size: 1630 4926 MfeI C'AATT_G Cuts at: 4155 4155 Size: 6556 MluI A'CGCG_T Cuts at: 6432 6432 Size: 6556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3594 5670 5854 1305 Size: 2289 2076 184 2007 Fragments arranged by size: 2289 2076 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 2957 3056 3851 10 Size: 54 1425 343 198 927 99 795 2715 Fragments arranged by size: 2715 1425 927 795 343 198 99 54 MslI CAynn'nnrTG Cuts at: 518 1166 2591 2786 3149 4514 4873 5032 518 Size: 648 1425 195 363 1365 359 159 2042 Fragments arranged by size: 2042 1425 1365 648 363 359 195 159 MspA1I CmG'CkG Cuts at: 1606 2203 2310 3163 3610 3662 3679 3805 3972 Size: 597 107 853 447 52 17 126 167 Cuts at: 3972 4649 5797 6042 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 853 677 597 447 245 167 126 107 52 17 NaeI GCC'GGC Cuts at: 2053 2053 Size: 6556 NcoI C'CATG_G Cuts at: 513 1065 3735 513 Size: 552 2670 3334 Fragments arranged by size: 3334 2670 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6556 NgoMIV G'CCGG_C Cuts at: 2051 2051 Size: 6556 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6556 NotI GC'GGCC_GC Cuts at: 4003 4003 Size: 6556 NspI r_CATG'y Cuts at: 1132 1229 2596 3559 3694 3914 5459 1132 Size: 97 1367 963 135 220 1545 2229 Fragments arranged by size: 2229 1545 1367 963 220 135 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6556 PciI A'CATG_T Cuts at: 2592 5455 2592 Size: 2863 3693 PflMI CCAn_nnn'nTGG Cuts at: 1108 2920 3063 3502 1108 Size: 1812 143 439 4162 Fragments arranged by size: 4162 1812 439 143 PmeI GTTT'AAAC Cuts at: 3860 3860 Size: 6556 PmlI CAC'GTG Cuts at: 3205 3304 3205 Size: 99 6457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2555 2587 4625 4657 6138 6170 2555 Size: 32 2038 32 1481 32 2941 Fragments arranged by size: 2941 2038 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3331 3511 3331 Size: 180 6376 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6556 PsiI TTA'TAA Cuts at: 4126 4126 Size: 6556 PspXI vC'TCGA_Gb Cuts at: 3758 3758 Size: 6556 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3527 3559 3527 Size: 32 6524 PstI C_TGCA'G Cuts at: 830 2102 3525 3908 830 Size: 1272 1423 383 3478 Fragments arranged by size: 3478 1423 1272 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6556 PvuII CAG'CTG Cuts at: 2203 3163 3610 3679 3805 2203 Size: 960 447 69 126 4954 Fragments arranged by size: 4954 960 447 126 69 SacII CC_GC'GG Cuts at: 3663 3663 Size: 6556 SalI G'TCGA_C Cuts at: 1936 3898 1936 Size: 1962 4594 SapI GCTCTTCn'nnn_ Cuts at: 3696 3696 Size: 6556 SbfI CC_TGCA'GG Cuts at: 3908 3908 Size: 6556 ScaI AGT'ACT Cuts at: 2228 4804 6516 2228 Size: 2576 1712 2268 Fragments arranged by size: 2576 2268 1712 SexAI A'CCwGG_T Cuts at: 3495 3495 Size: 6556 SfcI C'TryA_G Cuts at: 826 1045 2098 3521 3904 5039 5720 5911 826 Size: 219 1053 1423 383 1135 681 191 1471 Fragments arranged by size: 1471 1423 1135 1053 681 383 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3371 3371 Size: 6556 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 SgrAI Cr'CCGG_yG Cuts at: 1700 2318 1700 Size: 618 5938 SmaI CCC'GGG Cuts at: 1872 3000 3377 3883 1872 Size: 1128 377 506 4545 Fragments arranged by size: 4545 1128 506 377 SmlI C'TyrA_G Cuts at: 820 1017 1943 2387 2464 3758 4660 5561 5823 Size: 197 926 444 77 1294 902 901 262 Cuts at: 5823 820 Size: 1553 Fragments arranged by size: 1553 1294 926 902 901 444 262 197 77 SnaBI TAC'GTA Cuts at: 493 4347 493 Size: 3854 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6556 SphI G_CATG'C Cuts at: 1132 3559 3914 1132 Size: 2427 355 3774 Fragments arranged by size: 3774 2427 355 SspI AAT'ATT Cuts at: 5 52 4480 5 Size: 47 4428 2081 Fragments arranged by size: 4428 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6556 StyI C'CwwG_G Cuts at: 513 1065 3231 3735 3995 513 Size: 552 2166 504 260 3074 Fragments arranged by size: 3074 2166 552 504 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3055 3119 3580 4583 4600 4753 4938 Size: 1255 1475 64 461 1003 17 153 185 Cuts at: 4938 5383 325 Size: 445 1498 Fragments arranged by size: 1498 1475 1255 1003 461 445 185 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2256 4802 Size: 274 80 33 51 1443 249 30 2546 Cuts at: 4802 6514 96 Size: 1712 138 Fragments arranged by size: 2546 1712 1443 274 249 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2901 2933 6516 6548 2901 Size: 32 3583 32 2909 Fragments arranged by size: 3583 2909 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6556 XbaI T'CTAG_A Cuts at: 3892 3892 Size: 6556 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2717 2717 Size: 6556 XhoI C'TCGA_G Cuts at: 1943 3758 1943 Size: 1815 4741 XmaI C'CCGG_G Cuts at: 1870 2998 3375 3881 1870 Size: 1128 377 506 4545 Fragments arranged by size: 4545 1128 506 377 XmnI GAAnn'nnTTC Cuts at: 2570 2615 2849 4685 2570 Size: 45 234 1836 4441 Fragments arranged by size: 4441 1836 234 45 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4364 276 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI RsrII SacI SanDI SfoI SrfI SwaI