Restriction map for
pFN21AB6759
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# NO CUT ENZYMES ( 200 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BmyI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FauI FseI FspI HaeIII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tsp45I TspRI Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 155 AAATTC [GA]^AATT[CT]
AlwNI 166 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 85 CCTCAGG CC^T[ACGT]AGG
Aor51HI 45 AGCGCT AGC^GCT
ApoI 155 AAATTC [GA]^AATT[CT]
AvaII 300 GGACC G^G[AT]CC
BclI 143 TGATCA T^GATCA
BlpI 97 GCTCAGC GC^T[ACGT]AGC
BpmI 172 CTGGAG CTGGAG(16/14)
Bpu1102I 97 GCTCAGC GC^T[ACGT]AGC
BsiYI 165 CCAGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 165 CCAGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 127 ACCTGC ACCTGC(4/8)
BstF5I 198 CATCC GGATG(2/0)
Bsu36I 85 CCTCAGG CC^T[ACGT]AGG
Cac8I 56 GCCTGC GC[ACGT]^[ACGT]GC
CelII 97 GCTCAGC GC^T[ACGT]AGC
CfoI 46 GCGC GCG^C
Cfr13I 300 GGACC G^G[ACGT]CC
Eco47III 45 AGCGCT AGC^GCT
Eco81I 85 CCTCAGG CC^T[ACGT]AGG
FbaI 143 TGATCA T^GATCA
FokI 198 CATCC GGATG(9/13)
HaeII 45 AGCGCT [GA]GCGC^[CT]
HhaI 46 GCGC GCG^C
HinP1I 46 GCGC G^CGC
MaeII 140 ACGT A^CGT
MboII 224 TCTTC GAAGA(8/7)
MseI 137 TTAA T^TAA
PflMI 165 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 41 CTGCAG CTGCA^G
Sau96I 300 GGACC G^G[ACGT]CC
SfaNI 294 GCATC GCATC(5/9)
SfcI 41 CTGCAG C^T[GA][CT]AG
SspI 306 AATATT AAT^ATT
Tru9I 137 TTAA T^TAA
Tsp509I 156 AATT ^AATT
Van91I 165 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 287 CCATGAAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BsaJI 209 CCAAGG C^C[ACGT][ACGT]GG
263 CCAAGG
BsmFI 90 GGGAC GGGAC(10/14)
206 GTCCC
DdeI 86 CTCAG C^T[ACGT]AG
98 CTCAG
DpnII 144 GATC ^GATC
222 GATC
EcoT14I 209 CCAAGG C^C[AT][AT]GG
263 CCAAGG
HphI 125 TCACC GGTGA(8/7)
191 TCACC
MboI 144 GATC ^GATC
222 GATC
NdeII 144 GATC ^GATC
222 GATC
NlaIII 66 CATG CATG^
288 CATG
Sau3AI 144 GATC ^GATC
222 GATC
StyI 209 CCAAGG C^C[AT][AT]GG
263 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AluI BbvI Fnu4HI HinfI ItaI
MnlI TfiI TseI