(Linear) (Six-base) MAPSORT of: KIBB6759.seq  Check: 5820  from: 1  to: 327



KIBB6759.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     136     327 
   Size:        136     191
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     327 
   Size:         25     302
 
AfeI AGC'GCT

Cuts at:      0      47     327 
   Size:         47     280
 
AlwNI CAG_nnn'CTG

Cuts at:      0     171     327 
   Size:        171     156
 
ApoI r'AATT_y

Cuts at:      0     155     327 
   Size:        155     172
 
BclI T'GATC_A

Cuts at:      0     143     327 
   Size:        143     184
 
BlpI GC'TnA_GC

Cuts at:      0      98     327 
   Size:         98     229
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     327 
   Size:        193     134
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     136     327 
   Size:        136     191
 
Bsu36I CC'TnA_GG

Cuts at:      0      86     327 
   Size:         86     241
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     193     327 
   Size:         25     168     134

  Fragments arranged by size: 

                 168     134      25
 
HaeII r_GCGC'y

Cuts at:      0      49     327 
   Size:         49     278
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     171     327 
   Size:        171     156
 
PstI C_TGCA'G

Cuts at:      0      45     327 
   Size:         45     282
 
SfcI C'TryA_G

Cuts at:      0      41     327 
   Size:         41     286
 
SspI AAT'ATT

Cuts at:      0     308     327 
   Size:        308      19
 
StyI C'CwwG_G

Cuts at:      0     209     263     327 
   Size:        209      54      64

  Fragments arranged by size: 

                 209      64      54
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     294     327 
   Size:        294      33
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI     ApoI     BclI     BlpI     BpmI
    BspMI   Bsu36I  Eco57MI    HaeII    PflMI     PstI     SfcI     SspI
     StyI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI