################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SexAI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 6 CGCG CG^CG AocI 1264 CCTGAGG CC^T[ACGT]AGG ApaI 1095 GGGCCC GGGCC^C AvaII 979 GGACC G^G[AT]CC BanII 1095 GGGCCC G[GA]GC[CT]^C BbsI 286 GAAGAC GAAGAC(2/6) BcgI 1102 CGAAGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 892 GCCTCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 331 GCTCAGC GC^T[ACGT]AGC Bpu1102I 331 GCTCAGC GC^T[ACGT]AGC BpuAI 286 GAAGAC GAAGAC(2/6) BsaI 400 GAGACC GGTCTC(1/5) BsaWI 1286 ACCGGA [AT]^CCGG[AT] BsmBI 544 GAGACG CGTCTC(1/5) BsmI 1108 GAATGC GAATGC(1/-1) BspMI 692 ACCTGC ACCTGC(4/8) BssSI 772 CACGAG CACGAG(-5/-1) BstUI 6 CGCG CG^CG Bsu36I 1264 CCTGAGG CC^T[ACGT]AGG CelII 331 GCTCAGC GC^T[ACGT]AGC CfoI 783 GCGC GCG^C EaeI 413 TGGCCG [CT]^GGCC[GA] Eco81I 1264 CCTGAGG CC^T[ACGT]AGG EcoRV 172 GATATC GAT^ATC FauI 1115 GCGGG CCCGC(4/6) HaeII 782 GGCGCT [GA]GCGC^[CT] HgaI 4 GACGC GACGC(5/10) HhaI 783 GCGC GCG^C HinP1I 783 GCGC G^CGC MaeIII 1184 GTGAC ^GT[ACGT]AC MseI 154 TTAA T^TAA MvnI 6 CGCG CG^CG NspI 578 GCATGC [GA]CATG^[CT] PaeR7I 664 CTCGAG C^TCGAG PflMI 1272 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 694 CTGCAG CTGCA^G SbfI 693 CCTGCAGG CCTGCA^GG ScaI 650 AGTACT AGT^ACT SfiI 891 GGCCTCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 59 CCCGGG CCC^GGG SphI 578 GCATGC GCATG^C Sse8387I 693 CCTGCAGG CCTGCA^GG TfiI 477 GAATC G^A[AT]TC Tru9I 154 TTAA T^TAA Tsp45I 1184 GTGAC ^GT[GC]AC Van91I 1272 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 664 CTCGAG C^TCGAG XmaCI 59 CCCGGG C^CCGGG XmaI 59 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AlwNI 97 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 421 CAGCGGCTG AspHI 382 GTGCTC G[AT]GC[AT]^C 1311 GTGCTC BamHI 123 GGATCC G^GATCC 131 GGATCC BbvCI 1209 CCTCAGC CCTCAGC(-5/-2) 823 GCTGAGG BsgI 658 GTGCAG GTGCAG(16/14) 1354 GTGCAG BsiHKAI 382 GTGCTC G[AT]GC[AT]^C 1311 GTGCTC BsmFI 62 GGGAC GGGAC(10/14) 1059 GTCCC BstAPI 420 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 465 GCAGGAGATGC HphI 243 GGTGA GGTGA(8/7) 261 GGTGA NcoI 569 CCATGG C^CATGG 794 CCATGG Tsp509I 270 AATT ^AATT 1332 AATT XcmI 521 CCAGGAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 701 CCAAACTGACCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TseI TspRI TthHB8I XhoII