(Circular) (Six-base) MAPSORT of: pFN21AE1920.seq Check: 9139 from: 1 to: 6070 pFN21AE1920 6070 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3413 1937 Size: 1476 4594 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 2765 2961 4133 5517 5785 1632 Size: 407 726 196 1172 1384 268 1917 Fragments arranged by size: 1917 1384 1172 726 407 268 196 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 4969 5946 1203 Size: 3766 977 1327 Fragments arranged by size: 3766 1327 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 4799 4799 Size: 6070 AleI CACnn'nnGTG Cuts at: 1166 2384 2846 1166 Size: 1218 462 4390 Fragments arranged by size: 4390 1218 462 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AlwNI CAG_nnn'CTG Cuts at: 2107 2431 3813 5385 2107 Size: 324 1382 1572 2792 Fragments arranged by size: 2792 1572 1382 324 ApaI G_GGCC'C Cuts at: 3104 3104 Size: 6070 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 3379 3605 3757 5866 1493 Size: 1886 226 152 2109 1697 Fragments arranged by size: 2109 1886 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2064 2669 2808 3395 1870 Size: 73 121 605 139 587 4545 Fragments arranged by size: 4545 605 587 139 121 73 BamHI G'GATC_C Cuts at: 2128 2136 3400 3767 2128 Size: 8 1264 367 4431 Fragments arranged by size: 4431 1264 367 8 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3391 4846 618 Size: 325 217 627 1604 1455 1842 Fragments arranged by size: 1842 1604 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3104 3389 1391 Size: 1713 285 4072 Fragments arranged by size: 4072 1713 285 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2298 928 Size: 971 399 4700 Fragments arranged by size: 4700 971 399 BbvCI CC'TCA_GC Cuts at: 2829 3215 2829 Size: 386 5684 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3096 3130 4261 4295 1903 Size: 34 1159 34 1131 34 3678 Fragments arranged by size: 3678 1159 1131 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 1548 Size: 6070 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2903 4681 136 Size: 107 122 71 631 605 1231 1778 1525 Fragments arranged by size: 1778 1525 1231 631 605 122 107 71 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 2337 3487 2337 Size: 1150 4920 Bme1580I G_kGCm'C Cuts at: 1790 3104 4131 5287 1790 Size: 1314 1027 1156 2573 Fragments arranged by size: 2573 1314 1156 1027 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2317 2953 3086 Size: 416 53 83 399 24 900 636 133 Cuts at: 3086 3801 4759 442 Size: 715 958 1753 Fragments arranged by size: 1753 958 900 715 636 416 399 133 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2352 2428 2779 2352 Size: 76 351 5643 Fragments arranged by size: 5643 351 76 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2171 2456 2615 2774 2776 4730 1239 Size: 890 42 285 159 159 2 1954 2579 Fragments arranged by size: 2579 1954 890 285 159 159 42 2 Bpu10I CC'TnA_GC Cuts at: 2829 2874 3215 2829 Size: 45 341 5684 Fragments arranged by size: 5684 341 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2222 2591 2630 4195 5060 5358 2222 Size: 369 39 1565 865 298 2934 Fragments arranged by size: 2934 1565 865 369 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2399 4733 882 Size: 617 900 2334 2219 Fragments arranged by size: 2334 2219 900 617 BsaAI yAC'GTr Cuts at: 493 1204 3861 493 Size: 711 2657 2702 Fragments arranged by size: 2702 2657 711 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3877 4259 4894 275 Size: 53 83 186 936 2344 382 635 1451 Fragments arranged by size: 2344 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3291 4503 4901 5175 5322 5733 3291 Size: 1212 398 274 147 411 3628 Fragments arranged by size: 3628 1212 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5845 Fragments arranged by size: 5845 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2093 2096 2126 3118 3168 1186 Size: 865 42 3 30 992 50 4088 Fragments arranged by size: 4088 992 865 50 42 30 3 BseYI C'CCAG_C Cuts at: 3053 5273 5769 3053 Size: 2220 496 3354 Fragments arranged by size: 3354 2220 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 3380 1163 Size: 573 948 696 3853 Fragments arranged by size: 3853 948 696 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3520 4281 5309 1540 Size: 275 186 1519 761 1028 2301 Fragments arranged by size: 2301 1519 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2391 3320 4131 4216 5287 2391 Size: 929 811 85 1071 3174 Fragments arranged by size: 3174 1071 929 811 85 BsmI GAATG_Cn' Cuts at: 3119 3581 3674 3119 Size: 462 93 5515 Fragments arranged by size: 5515 462 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2543 2543 Size: 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2391 3104 3320 3389 4131 4216 5287 Size: 399 601 713 216 69 742 85 1071 Cuts at: 5287 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 713 601 399 216 85 69 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2706 3425 844 Size: 1862 719 3489 Fragments arranged by size: 3489 1862 719 BsrBI CCG'CTC Cuts at: 1394 1823 2031 2226 2782 3957 1394 Size: 429 208 195 556 1175 3507 Fragments arranged by size: 3507 1175 556 429 208 195 BsrDI GCAATG_nn' Cuts at: 65 1248 4565 4739 65 Size: 1183 3317 174 1396 Fragments arranged by size: 3317 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4714 4901 1700 Size: 110 2904 187 2869 Fragments arranged by size: 2904 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 2777 4130 5142 2777 Size: 1353 1012 3705 Fragments arranged by size: 3705 1353 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2431 2476 794 Size: 1637 45 4388 Fragments arranged by size: 4388 1637 45 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6070 BstXI CCAn_nnnn'nTGG Cuts at: 2416 2800 2893 2416 Size: 384 93 5593 Fragments arranged by size: 5593 384 93 BstYI r'GATC_y Cuts at: 1972 2128 2136 2772 2854 3046 3400 3767 4152 Size: 156 8 636 82 192 354 367 385 Cuts at: 4152 4169 5621 5707 5719 6065 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 636 385 367 354 346 192 156 86 82 17 12 8 Bsu36I CC'TnA_GG Cuts at: 3270 3270 Size: 6070 BtgI C'CryG_G Cuts at: 513 1065 2004 2169 2574 2799 513 Size: 552 939 165 405 225 3784 Fragments arranged by size: 3784 939 552 405 225 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 3232 3238 3586 4380 4400 3232 Size: 6 348 794 20 4902 Fragments arranged by size: 4902 794 348 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 3374 3374 Size: 6070 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5077 809 Size: 507 3761 1802 Fragments arranged by size: 3761 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2418 3517 4406 Size: 54 1425 50 275 18 588 1099 889 Cuts at: 4406 6035 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1099 889 588 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3517 1537 Size: 275 1705 4090 Fragments arranged by size: 4090 1705 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 2082 2115 3322 4000 1353 Size: 717 12 33 1207 678 3423 Fragments arranged by size: 3423 1207 717 678 33 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2452 2525 4657 5031 5177 387 Size: 1064 138 863 73 2132 374 146 1280 Fragments arranged by size: 2132 1280 1064 863 374 146 138 73 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2129 2171 2456 2615 2765 2774 Size: 393 407 90 42 285 159 150 9 Cuts at: 2774 2776 2961 4133 4730 5517 5785 1239 Size: 2 185 1172 597 787 268 1524 Fragments arranged by size: 1524 1172 787 597 407 393 285 268 185 159 150 90 42 9 2 EcoO109I rG'GnC_Cy Cuts at: 2804 3101 3123 3211 2804 Size: 297 22 88 5663 Fragments arranged by size: 5663 297 88 22 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 EcoRV GAT'ATC Cuts at: 2179 2179 Size: 6070 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 2791 5217 1678 Size: 1113 2426 2531 Fragments arranged by size: 2531 2426 1113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2467 2499 4711 4743 4785 Size: 32 135 32 895 32 2212 32 42 Cuts at: 4785 4817 1373 Size: 32 2626 Fragments arranged by size: 2626 2212 895 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3414 3660 669 Size: 988 281 1476 246 3079 Fragments arranged by size: 3079 1476 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5184 5368 1305 Size: 3879 184 2007 Fragments arranged by size: 3879 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4248 Fragments arranged by size: 4248 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 2846 2891 3353 4028 4387 4546 Size: 648 1218 462 45 462 675 359 159 Cuts at: 4546 518 Size: 2042 Fragments arranged by size: 2042 1218 675 648 462 462 359 159 45 MspA1I CmG'CkG Cuts at: 1606 2341 2428 2593 2922 3120 3486 4163 5311 Size: 735 87 165 329 198 366 677 1148 Cuts at: 5311 5556 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 735 677 366 329 245 198 165 87 NcoI C'CATG_G Cuts at: 513 1065 2004 2574 2799 513 Size: 552 939 570 225 3784 Fragments arranged by size: 3784 939 570 552 225 NdeI CA'TA_TG Cuts at: 387 387 Size: 6070 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 2587 3428 4973 1132 Size: 97 1358 841 1545 2229 Fragments arranged by size: 2229 1545 1358 841 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6070 PciI A'CATG_T Cuts at: 4969 4969 Size: 6070 PflMI CCAn_nnn'nTGG Cuts at: 1108 3283 1108 Size: 2175 3895 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6070 PsiI TTA'TAA Cuts at: 3640 3640 Size: 6070 PspOMI G'GGCC_C Cuts at: 3100 3100 Size: 6070 PspXI vC'TCGA_Gb Cuts at: 2669 2669 Size: 6070 PstI C_TGCA'G Cuts at: 830 2703 3422 830 Size: 1873 719 3478 Fragments arranged by size: 3478 1873 719 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 PvuII CAG'CTG Cuts at: 2341 2593 2922 2341 Size: 252 329 5489 Fragments arranged by size: 5489 329 252 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SbfI CC_TGCA'GG Cuts at: 2703 3422 2703 Size: 719 5351 ScaI AGT'ACT Cuts at: 2657 4318 6030 2657 Size: 1661 1712 2697 Fragments arranged by size: 2697 1712 1661 SfcI C'TryA_G Cuts at: 826 1045 2699 2923 3311 3418 4553 5234 5425 Size: 219 1654 224 388 107 1135 681 191 Cuts at: 5425 826 Size: 1471 Fragments arranged by size: 1654 1471 1135 681 388 224 219 191 107 SfiI GGCCn_nnn'nGGCC Cuts at: 2903 2903 Size: 6070 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 2066 3397 1872 Size: 194 1331 4545 Fragments arranged by size: 4545 1331 194 SmlI C'TyrA_G Cuts at: 820 1017 1943 2201 2606 2645 2669 4174 5075 Size: 197 926 258 405 39 24 1505 901 Cuts at: 5075 5337 820 Size: 262 1553 Fragments arranged by size: 1553 1505 926 901 405 262 258 197 39 24 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 2587 3428 1132 Size: 1455 841 3774 Fragments arranged by size: 3774 1455 841 SspI AAT'ATT Cuts at: 5 52 3994 5 Size: 47 3942 2081 Fragments arranged by size: 3942 2081 47 StuI AGG'CCT Cuts at: 1517 2906 3034 3281 1517 Size: 1389 128 247 4306 Fragments arranged by size: 4306 1389 247 128 StyI C'CwwG_G Cuts at: 513 1065 2004 2400 2574 2799 3282 3509 513 Size: 552 939 396 174 225 483 227 3074 Fragments arranged by size: 3074 939 552 483 396 227 225 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4097 4114 4267 4452 4897 325 Size: 1255 2517 17 153 185 445 1498 Fragments arranged by size: 2517 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 2730 4316 Size: 274 80 33 51 1443 678 75 1586 Cuts at: 4316 6028 96 Size: 1712 138 Fragments arranged by size: 1712 1586 1443 678 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6030 6062 6030 Size: 32 6038 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2533 2713 2533 Size: 180 5890 XhoI C'TCGA_G Cuts at: 1943 2669 1943 Size: 726 5344 XmaI C'CCGG_G Cuts at: 1870 2064 3395 1870 Size: 194 1331 4545 Fragments arranged by size: 4545 1331 194 XmnI GAAnn'nnTTC Cuts at: 4199 4199 Size: 6070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI