################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MfeI MluI MroI MunI NarI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI TaqI TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 235 GACGTC GACGT^C AcyI 235 GACGTC G[GA]^CG[CT]C AlwI 1110 GATCC GGATC(4/5) AlwNI 256 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 907 CCTGAGG CC^T[ACGT]AGG AscI 437 GGCGCGCC GG^CGCGCC AspI 232 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 814 GGCACC G^G[CT][GA]CC BfaI 457 CTAG C^TAG BpmI 187 CTGGAG CTGGAG(16/14) BsaBI 64 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 235 GACGTC G[GA]^CG[CT]C BsaWI 1051 ACCGGA [AT]^CCGG[AT] BsmFI 167 GGGAC GGGAC(10/14) BsrDI 1031 GCAATG GCAATG(2/0) BstYI 1109 AGATCC [GA]^GATC[CT] Bsu36I 907 CCTGAGG CC^T[ACGT]AGG DraII 125 AGGACCC [GA]G^G[ACGT]CC[CT] Eco81I 907 CCTGAGG CC^T[ACGT]AGG EcoNI 907 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 125 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 822 GAATTC G^AATTC EcoT14I 779 CCATGG C^C[AT][AT]GG EcoT22I 1 ATGCAT ATGCA^T HaeII 419 AGCGCC [GA]GCGC^[CT] Hin1I 235 GACGTC G[GA]^CG[CT]C HphI 859 GGTGA GGTGA(8/7) KspI 449 CCGCGG CCGC^GG MaeI 457 CTAG C^TAG MaeII 236 ACGT A^CGT MamI 64 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1109 AGATCC [GA]^GATC[CT] MseI 655 TTAA T^TAA NcoI 779 CCATGG C^CATGG NdeI 698 CATATG CA^TATG NsiI 1 ATGCAT ATGCA^T NspI 212 GCATGC [GA]CATG^[CT] PflFI 232 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 125 AGGACCC [GA]G^G[AT]CC[CT] PvuII 709 CAGCTG CAG^CTG SacI 1127 GAGCTC GAGCT^C SacII 449 CCGCGG CCGC^GG SapI 174 GCTCTTC GCTCTTC(1/4) SmlI 610 CTTGAG C^T[CT][GA]AG SphI 212 GCATGC GCATG^C SspI 1084 AATATT AAT^ATT StyI 779 CCATGG C^C[AT][AT]GG Tru9I 655 TTAA T^TAA Tth111I 232 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1109 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 84 GAATTT [GA]^AATT[CT] 822 GAATTC ApoI 84 GAATTT [GA]^AATT[CT] 822 GAATTC AvaII 126 GGACC G^G[AT]CC 800 GGACC BalI 887 TGGCCA TGG^CCA 930 TGGCCA BsaI 597 GAGACC GGTCTC(1/5) 1048 GAGACC BsgI 487 CTGCAC GTGCAG(16/14) 496 GTGCAG BspMI 485 ACCTGC ACCTGC(4/8) 529 GCAGGT BsrFI 278 GCCGGC [GA]^CCGG[CT] 525 GCCGGC BssHII 308 GCGCGC G^CGCGC 438 GCGCGC BstAPI 255 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 489 GCACGCGGTGC BstXI 341 CCAGTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1113 CCAGCCACATGG Cfr10I 278 GCCGGC [GA]^CCGG[CT] 525 GCCGGC DpnII 70 GATC ^GATC 1110 GATC DraIII 132 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG 490 CACGCGGTG EarI 175 CTCTTC CTCTTC(1/4) 1036 GAAGAG HgaI 376 GACGC GACGC(5/10) 474 GACGC Ksp632I 175 CTCTTC CTCTTC(1/4) 1036 GAAGAG MboI 70 GATC ^GATC 1110 GATC MluNI 887 TGGCCA TGG^CCA 930 TGGCCA MscI 887 TGGCCA TGG^CCA 930 TGGCCA MslI 75 CATGTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 842 CATCCTTGTG MspA1I 449 CCGCGG C[AC]G^C[GT]G 709 CAGCTG NaeI 278 GCCGGC GCC^GGC 525 GCCGGC NdeII 70 GATC ^GATC 1110 GATC NgoMIV 278 GCCGGC G^CCGGC 525 GCCGGC Sau3AI 70 GATC ^GATC 1110 GATC SfaNI 3 GCATC GCATC(5/9) 588 GCATC Tsp509I 85 AATT ^AATT 823 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AluI AspHI BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeIII MboII MnlI MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI Sau96I ScrFI SfcI TfiI TseI Tsp45I TspRI XcmI