(Circular) (Six-base) MAPSORT of: pFN21AE1862.seq Check: 7758 from: 1 to: 5848 pFN21AE1862 5848 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2244 3658 278 Size: 53 83 186 936 708 1414 2468 Fragments arranged by size: 2468 1414 936 708 186 83 53 AccI GT'mk_AC Cuts at: 1937 3191 1937 Size: 1254 4594 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2799 3911 5295 5563 1632 Size: 1167 1112 1384 268 1917 Fragments arranged by size: 1917 1384 1167 1112 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 4747 5724 1203 Size: 3544 977 1327 Fragments arranged by size: 3544 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2031 2240 1184 Size: 847 209 4792 Fragments arranged by size: 4792 847 209 AlwNI CAG_nnn'CTG Cuts at: 2266 3591 5163 2266 Size: 1325 1572 2951 Fragments arranged by size: 2951 1572 1325 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 2089 2827 3157 3383 3535 5644 1493 Size: 596 738 330 226 152 2109 1697 Fragments arranged by size: 2109 1697 738 596 330 226 152 AscI GG'CGCG_CC Cuts at: 2443 2443 Size: 5848 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 3173 1870 Size: 73 1230 4545 Fragments arranged by size: 4545 1230 73 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2819 3169 4624 618 Size: 325 217 627 1032 350 1455 1842 Fragments arranged by size: 1842 1455 1032 627 350 325 217 BanII G_rGCy'C Cuts at: 1391 2326 2844 3136 3167 1391 Size: 935 518 292 31 4072 Fragments arranged by size: 4072 935 518 292 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4039 4073 1903 Size: 34 50 34 2018 34 3678 Fragments arranged by size: 3678 2018 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4459 136 Size: 107 122 71 631 605 2787 1525 Fragments arranged by size: 2787 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 3909 5065 1790 Size: 2119 1156 2573 Fragments arranged by size: 2573 2119 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3579 4537 442 Size: 416 53 83 399 24 2162 958 1753 Fragments arranged by size: 2162 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 4508 1239 Size: 974 2295 2579 Fragments arranged by size: 2579 2295 974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2636 3973 4838 5136 2636 Size: 1337 865 298 3348 Fragments arranged by size: 3348 1337 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2596 3047 4511 882 Size: 617 1097 451 1464 2219 Fragments arranged by size: 2219 1464 1097 617 451 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 2073 5842 2073 Size: 3769 2079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2241 3655 4037 4672 Size: 53 83 186 936 708 1414 382 635 Cuts at: 4672 275 Size: 1451 Fragments arranged by size: 1451 1414 936 708 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3056 4281 4679 4953 5100 5511 3056 Size: 1225 398 274 147 411 3393 Fragments arranged by size: 3393 1225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5623 Fragments arranged by size: 5623 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2447 2480 2774 3075 3144 1186 Size: 1102 159 33 294 301 69 3890 Fragments arranged by size: 3890 1102 301 294 159 69 33 BseYI C'CCAG_C Cuts at: 2278 2508 5051 5547 2278 Size: 230 2543 496 2579 Fragments arranged by size: 2579 2543 496 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2477 2522 1163 Size: 573 741 45 4489 Fragments arranged by size: 4489 741 573 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3298 4059 5087 1540 Size: 275 186 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2035 2163 2353 3136 3909 3994 5065 2035 Size: 128 190 783 773 85 1071 2818 Fragments arranged by size: 2818 1071 783 773 190 128 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2163 2326 2353 2844 3136 3167 Size: 399 245 128 163 27 491 292 31 Cuts at: 3167 3909 3994 5065 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 491 399 292 245 163 128 85 31 27 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2499 2525 3203 844 Size: 1655 26 678 3489 Fragments arranged by size: 3489 1655 678 26 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3043 4343 4517 65 Size: 1183 1795 1300 174 1396 Fragments arranged by size: 1795 1396 1300 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2283 2530 4492 4679 1700 Size: 110 473 247 1962 187 2869 Fragments arranged by size: 2869 1962 473 247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 2313 2443 1925 Size: 388 130 5330 Fragments arranged by size: 5330 388 130 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 2266 2500 794 Size: 1472 234 4142 Fragments arranged by size: 4142 1472 234 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstXI CCAn_nnnn'nTGG Cuts at: 2353 3125 2353 Size: 772 5076 BstYI r'GATC_y Cuts at: 1972 3114 3178 3545 3930 3947 5399 5485 5497 Size: 1142 64 367 385 17 1452 86 12 Cuts at: 5497 5843 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1142 385 367 346 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2913 2913 Size: 5848 BtgI C'CryG_G Cuts at: 513 1065 2232 2454 2784 513 Size: 552 1167 222 330 3577 Fragments arranged by size: 3577 1167 552 330 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 2052 3364 4158 4178 2052 Size: 1312 794 20 3722 Fragments arranged by size: 3722 1312 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DraIII CAC_nnn'GTG Cuts at: 2142 2500 2142 Size: 358 5490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2451 2892 Size: 54 1425 50 275 18 222 399 441 Cuts at: 2892 2935 3295 4184 5813 8 Size: 43 360 889 1629 43 Fragments arranged by size: 1629 1425 889 441 399 360 275 222 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 3036 3778 1353 Size: 833 850 742 3423 Fragments arranged by size: 3423 850 833 742 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3134 3134 Size: 5848 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2799 3911 4508 5295 5563 1239 Size: 393 581 586 1112 597 787 268 1524 Fragments arranged by size: 1524 1112 787 597 586 581 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2916 2916 Size: 5848 EcoO109I rG'GnC_Cy Cuts at: 2131 2131 Size: 5848 EcoRI G'AATT_C Cuts at: 2827 3157 3535 2827 Size: 330 378 5140 Fragments arranged by size: 5140 378 330 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2428 4995 1678 Size: 750 2567 2531 Fragments arranged by size: 2567 2531 750 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2335 2367 4489 4521 4563 Size: 32 135 32 763 32 2122 32 42 Cuts at: 4563 4595 1373 Size: 32 2626 Fragments arranged by size: 2626 2122 763 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3192 3438 669 Size: 988 281 1254 246 3079 Fragments arranged by size: 3079 1254 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4962 5146 1305 Size: 3657 184 2007 Fragments arranged by size: 3657 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2894 2937 10 Size: 54 1425 343 1062 43 2921 Fragments arranged by size: 2921 1425 1062 343 54 43 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2851 3806 4165 4324 518 Size: 648 918 767 955 359 159 2042 Fragments arranged by size: 2042 955 918 767 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2456 2716 3264 3941 5089 5334 1606 Size: 850 260 548 677 1148 245 2120 Fragments arranged by size: 2120 1148 850 677 548 260 245 NaeI GCC'GGC Cuts at: 2285 2532 2285 Size: 247 5601 NcoI C'CATG_G Cuts at: 513 1065 2784 513 Size: 552 1719 3577 Fragments arranged by size: 3577 1719 552 NdeI CA'TA_TG Cuts at: 387 2704 387 Size: 2317 3531 NgoMIV G'CCGG_C Cuts at: 2283 2530 2283 Size: 247 5601 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2221 3206 4751 1132 Size: 97 992 985 1545 2229 Fragments arranged by size: 2229 1545 992 985 97 PasI CC'CwG_GG Cuts at: 1348 2794 1348 Size: 1446 4402 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2131 2131 Size: 5848 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PstI C_TGCA'G Cuts at: 830 2047 2262 2370 2931 3200 830 Size: 1217 215 108 561 269 3478 Fragments arranged by size: 3478 1217 561 269 215 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2716 2716 Size: 5848 SacI G_AGCT'C Cuts at: 3136 3136 Size: 5848 SacII CC_GC'GG Cuts at: 2457 2457 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SapI GCTCTTCn'nnn_ Cuts at: 2186 2186 Size: 5848 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SfcI C'TryA_G Cuts at: 826 1045 2043 2258 2366 2927 3196 4331 5012 Size: 219 998 215 108 561 269 1135 681 Cuts at: 5012 5203 826 Size: 191 1471 Fragments arranged by size: 1471 1135 998 681 561 269 219 215 191 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2615 3952 4853 5115 820 Size: 197 926 672 1337 901 262 1553 Fragments arranged by size: 1553 1337 926 901 672 262 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 2221 3206 1132 Size: 1089 985 3774 Fragments arranged by size: 3774 1089 985 SspI AAT'ATT Cuts at: 5 52 3091 3772 5 Size: 47 3039 681 2081 Fragments arranged by size: 3039 2081 681 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2784 3287 513 Size: 552 1719 503 3074 Fragments arranged by size: 3074 1719 552 503 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2741 2951 3087 3875 3892 4045 4230 Size: 1255 1161 210 136 788 17 153 185 Cuts at: 4230 4675 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1161 788 445 210 185 153 136 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4094 5806 96 Size: 274 80 33 51 1443 2117 1712 138 Fragments arranged by size: 2117 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5808 5840 5808 Size: 32 5816 Tth111I GACn'n_nGTC Cuts at: 1316 2240 1316 Size: 924 4924 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2743 2791 2887 2945 2743 Size: 48 96 58 5646 Fragments arranged by size: 5646 96 58 48 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2242 3656 276 Size: 53 83 186 936 708 1414 2468 Fragments arranged by size: 2468 1414 936 708 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI