(Linear) (Six-base) MAPSORT of: KIEE1862.seq Check: 7734 from: 1 to: 1143 KIEE1862.orf With 182 enzymes: SgfI * July 15, 2010 18:49 .. AatII G_ACGT'C Cuts at: 0 239 1143 Size: 239 904 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 794 1143 Size: 794 349 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 26 235 1143 Size: 26 209 908 Fragments arranged by size: 908 209 26 AlwNI CAG_nnn'CTG Cuts at: 0 261 1143 Size: 261 882 ApoI r'AATT_y Cuts at: 0 84 822 1143 Size: 84 738 321 Fragments arranged by size: 738 321 84 AscI GG'CGCG_CC Cuts at: 0 438 1143 Size: 438 705 BanI G'GyrC_C Cuts at: 0 814 1143 Size: 814 329 BanII G_rGCy'C Cuts at: 0 321 839 1131 1143 Size: 321 518 292 12 Fragments arranged by size: 518 321 292 12 BfrBI ATG'CAT Cuts at: 0 3 1143 Size: 3 1140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 1143 Size: 208 935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 1143 Size: 631 512 BsaI GGTCTCn'nnnn_ Cuts at: 0 591 1042 1143 Size: 591 451 101 Fragments arranged by size: 591 451 101 BsaBI GATnn'nnATC Cuts at: 0 68 1143 Size: 68 1075 BsaHI Gr'CG_yC Cuts at: 0 236 1143 Size: 236 907 BsaWI w'CCGG_w Cuts at: 0 1051 1143 Size: 1051 92 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 283 442 475 769 1070 1139 1143 Size: 283 159 33 294 301 69 4 Fragments arranged by size: 301 294 283 159 69 33 4 BseYI C'CCAG_C Cuts at: 0 273 503 1143 Size: 273 230 640 Fragments arranged by size: 640 273 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 517 1143 Size: 472 45 626 Fragments arranged by size: 626 472 45 BsiHKAI G_wGCw'C Cuts at: 0 30 158 348 1131 1143 Size: 30 128 190 783 12 Fragments arranged by size: 783 190 128 30 12 Bsp1286I G_dGCh'C Cuts at: 0 30 158 321 348 839 1131 1143 Size: 30 128 163 27 491 292 12 Fragments arranged by size: 491 292 163 128 30 27 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 494 520 1143 Size: 494 26 623 Fragments arranged by size: 623 494 26 BsrDI GCAATG_nn' Cuts at: 0 1038 1143 Size: 1038 105 BsrFI r'CCGG_y Cuts at: 0 278 525 1143 Size: 278 247 618 Fragments arranged by size: 618 278 247 BssHII G'CGCG_C Cuts at: 0 308 438 1143 Size: 308 130 705 Fragments arranged by size: 705 308 130 BstAPI GCAn_nnn'nTGC Cuts at: 0 261 495 1143 Size: 261 234 648 Fragments arranged by size: 648 261 234 BstXI CCAn_nnnn'nTGG Cuts at: 0 348 1120 1143 Size: 348 772 23 Fragments arranged by size: 772 348 23 BstYI r'GATC_y Cuts at: 0 1109 1143 Size: 1109 34 Bsu36I CC'TnA_GG Cuts at: 0 908 1143 Size: 908 235 BtgI C'CryG_G Cuts at: 0 227 449 779 1143 Size: 227 222 330 364 Fragments arranged by size: 364 330 227 222 BtsI GCAGTG_nn' Cuts at: 0 47 1143 Size: 47 1096 DraIII CAC_nnn'GTG Cuts at: 0 137 495 1143 Size: 137 358 648 Fragments arranged by size: 648 358 137 EaeI y'GGCC_r Cuts at: 0 47 446 887 930 1143 Size: 47 399 441 43 213 Fragments arranged by size: 441 399 213 47 43 EarI CTCTTCn'nnn_ Cuts at: 0 181 1031 1143 Size: 181 850 112 Fragments arranged by size: 850 181 112 EcoICRI GAG'CTC Cuts at: 0 1129 1143 Size: 1129 14 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 794 1143 Size: 208 586 349 Fragments arranged by size: 586 349 208 EcoNI CCTnn'n_nnAGG Cuts at: 0 911 1143 Size: 911 232 EcoO109I rG'GnC_Cy Cuts at: 0 126 1143 Size: 126 1017 EcoRI G'AATT_C Cuts at: 0 822 1143 Size: 822 321 HaeII r_GCGC'y Cuts at: 0 423 1143 Size: 423 720 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 330 362 1143 Size: 330 32 781 Fragments arranged by size: 781 330 32 MscI TGG'CCA Cuts at: 0 889 932 1143 Size: 889 43 211 Fragments arranged by size: 889 211 43 MslI CAynn'nnrTG Cuts at: 0 79 846 1143 Size: 79 767 297 Fragments arranged by size: 767 297 79 MspA1I CmG'CkG Cuts at: 0 451 711 1143 Size: 451 260 432 Fragments arranged by size: 451 432 260 NaeI GCC'GGC Cuts at: 0 280 527 1143 Size: 280 247 616 Fragments arranged by size: 616 280 247 NcoI C'CATG_G Cuts at: 0 779 1143 Size: 779 364 NdeI CA'TA_TG Cuts at: 0 699 1143 Size: 699 444 NgoMIV G'CCGG_C Cuts at: 0 278 525 1143 Size: 278 247 618 Fragments arranged by size: 618 278 247 NsiI A_TGCA'T Cuts at: 0 5 1143 Size: 5 1138 NspI r_CATG'y Cuts at: 0 216 1143 Size: 216 927 PasI CC'CwG_GG Cuts at: 0 789 1143 Size: 789 354 PpuMI rG'GwC_Cy Cuts at: 0 126 1143 Size: 126 1017 PstI C_TGCA'G Cuts at: 0 42 257 365 926 1143 Size: 42 215 108 561 217 Fragments arranged by size: 561 217 215 108 42 PvuII CAG'CTG Cuts at: 0 711 1143 Size: 711 432 SacI G_AGCT'C Cuts at: 0 1131 1143 Size: 1131 12 SacII CC_GC'GG Cuts at: 0 452 1143 Size: 452 691 SapI GCTCTTCn'nnn_ Cuts at: 0 181 1143 Size: 181 962 SfcI C'TryA_G Cuts at: 0 38 253 361 922 1143 Size: 38 215 108 561 221 Fragments arranged by size: 561 221 215 108 38 SmlI C'TyrA_G Cuts at: 0 610 1143 Size: 610 533 SphI G_CATG'C Cuts at: 0 216 1143 Size: 216 927 SspI AAT'ATT Cuts at: 0 1086 1143 Size: 1086 57 StyI C'CwwG_G Cuts at: 0 779 1143 Size: 779 364 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 736 946 1082 1143 Size: 736 210 136 61 Fragments arranged by size: 736 210 136 61 Tth111I GACn'n_nGTC Cuts at: 0 235 1143 Size: 235 908 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 738 786 882 940 1143 Size: 738 48 96 58 203 Fragments arranged by size: 738 203 96 58 48 ZraI GAC'GTC Cuts at: 0 237 1143 Size: 237 906 Enzymes that do cut: AatII AcuI AlfI AlwNI ApoI AscI BanI BanII BfrBI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PasI PpuMI PstI PvuII SacI SacII SapI SfcI SmlI SphI SspI StyI TaqII Tth111I XcmI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NheI NotI NruI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TatI TstI XbaI XhoI XmaI XmnI