Restriction map for
pFN21AE2067
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BfrI BglI BlnI BlpI
Bpu1102I BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HaeII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I RsrII SacI SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SspBI
SspI SwaI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 2122 AAATTC [GA]^AATT[CT]
AcyI 336 GACGCC G[GA]^CG[CT]C
AflIII 1142 ACACGT A^C[GA][CT]GT
AocI 514 CCTAAGG CC^T[ACGT]AGG
ApoI 2122 AAATTC [GA]^AATT[CT]
AspHI 1047 GAGCAC G[AT]GC[AT]^C
BbrPI 1380 CACGTG CAC^GTG
BbvCI 1983 CCTCAGC CCTCAGC(-5/-2)
BciVI 1629 GGATAC GTATCC(6/5)
BfaI 1729 CTAG C^TAG
BglII 914 AGATCT A^GATCT
BsaBI 1428 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 336 GACGCC G[GA]^CG[CT]C
BsiEI 807 CGATCG CG[GA][CT]^CG
BsiHKAI 1047 GAGCAC G[AT]GC[AT]^C
BspHI 1103 TCATGA T^CATGA
BsrBI 388 GAGCGG CCGCTC(-3/-3)
BsrDI 1569 CATTGC GCAATG(2/0)
BstEII 2058 GGTCACC G^GT[ACGT]ACC
BstPI 2058 GGTCACC G^GT[ACGT]ACC
Bsu36I 514 CCTAAGG CC^T[ACGT]AGG
DrdI 460 GACACCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 514 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2058 GGTCACC G^GT[ACGT]ACC
Hin1I 336 GACGCC G[GA]^CG[CT]C
HincII 1945 GTCAAC GT[CT]^[GA]AC
HindII 1945 GTCAAC GT[CT]^[GA]AC
MaeI 1729 CTAG C^TAG
MamI 1428 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 867 CCATGG C^CATGG
PmaCI 1380 CACGTG CAC^GTG
PmlI 1380 CACGTG CAC^GTG
PvuI 807 CGATCG CGAT^CG
RcaI 1103 TCATGA T^CATGA
SbfI 924 CCTGCAGG CCTGCA^GG
ScaI 1482 AGTACT AGT^ACT
Sse8387I 924 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 418 GTCTAC GT^[AC][GT]AC
1593 GTCTAC
AccII 157 CGCG CG^CG
270 CGCG
AlwNI 594 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1552 CAGCTCCTG
ApaI 254 GGGCCC GGGCC^C
446 GGGCCC
AvaI 19 CCCGAG C^[CT]CG[GA]G
85 CCCGAG
BalI 281 TGGCCA TGG^CCA
377 TGGCCA
BcgI 700 GCAGCGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1065 GCAGAAGAGTCG
BclI 506 TGATCA T^GATCA
554 TGATCA
BsaAI 1143 CACGTA [CT]AC^GT[GA]
1380 CACGTG
BsoBI 19 CCCGAG C^[CT]CG[GA]G
85 CCCGAG
BsrFI 690 ACCGGC [GA]^CCGG[CT]
1010 ACCGGC
BstAPI 593 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1780 GCAAAGTCTGC
BstUI 157 CGCG CG^CG
270 CGCG
BstYI 848 AGATCC [GA]^GATC[CT]
914 AGATCT
Cfr10I 690 ACCGGC [GA]^CCGG[CT]
1010 ACCGGC
DsaI 867 CCATGG C^C[GA][CT]GG
1205 CCACGG
FbaI 506 TGATCA T^GATCA
554 TGATCA
MflI 848 AGATCC [GA]^GATC[CT]
914 AGATCT
MluNI 281 TGGCCA TGG^CCA
377 TGGCCA
MscI 281 TGGCCA TGG^CCA
377 TGGCCA
MvnI 157 CGCG CG^CG
270 CGCG
SapI 1578 GAAGAGC GCTCTTC(1/4)
1762 GAAGAGC
SphI 1263 GCATGC GCATG^C
1421 GCATGC
StuI 204 AGGCCT AGG^CCT
1992 AGGCCT
TaqI 1436 TCGA T^CGA
1506 TCGA
Tsp509I 1451 AATT ^AATT
2123 AATT
TthHB8I 1436 TCGA T^CGA
1506 TCGA
XhoII 848 AGATCC [GA]^GATC[CT]
914 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I TspRI Van91I