(Circular) (Six-base) MAPSORT of: pFN21AE2067.seq Check: 7469 from: 1 to: 6883 pFN21AE2067 6883 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2694 2855 2937 3790 3805 2694 Size: 161 82 853 15 5772 Fragments arranged by size: 5772 853 161 82 15 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4693 278 Size: 53 83 186 936 3157 2468 Fragments arranged by size: 3157 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2424 3599 4226 1937 Size: 487 1175 627 4594 Fragments arranged by size: 4594 1175 627 487 Acc65I G'GTAC_C Cuts at: 4204 4204 Size: 6883 AclI AA'CG_TT Cuts at: 1563 5010 5383 1563 Size: 3447 373 3063 Fragments arranged by size: 3447 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3658 4946 6330 6598 1632 Size: 2026 1288 1384 268 1917 Fragments arranged by size: 2026 1917 1384 1288 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6686 AflIII A'CryG_T Cuts at: 1203 3147 5782 6759 1203 Size: 1944 2635 977 1327 Fragments arranged by size: 2635 1944 1327 977 AgeI A'CCGG_T Cuts at: 5714 5714 Size: 6883 AhdI GACnn_n'nnGTC Cuts at: 5612 5612 Size: 6883 AleI CACnn'nnGTG Cuts at: 1166 3189 1166 Size: 2023 4860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3443 1184 Size: 2259 4624 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2231 2263 2231 Size: 32 6851 AlwNI CAG_nnn'CTG Cuts at: 2604 3562 4626 6198 2604 Size: 958 1064 1572 3289 Fragments arranged by size: 3289 1572 1064 958 ApaI G_GGCC'C Cuts at: 2263 2455 2263 Size: 192 6691 ApaLI G'TGCA_C Cuts at: 4940 6096 4940 Size: 1156 5727 ApoI r'AATT_y Cuts at: 1493 4127 4192 4418 4570 6679 1493 Size: 2634 65 226 152 2109 1697 Fragments arranged by size: 2634 2109 1697 226 152 65 AseI AT'TA_AT Cuts at: 160 1032 5437 160 Size: 872 4405 1606 Fragments arranged by size: 4405 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6883 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2090 4208 1870 Size: 73 81 66 2118 4545 Fragments arranged by size: 4545 2118 81 73 66 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2939 2972 2939 Size: 33 6850 BamHI G'GATC_C Cuts at: 4213 4580 4213 Size: 367 6516 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3242 3250 3661 4204 5659 Size: 325 217 627 1455 8 411 543 1455 Cuts at: 5659 618 Size: 1842 Fragments arranged by size: 1842 1455 1455 627 543 411 325 217 8 BanII G_rGCy'C Cuts at: 1391 2263 2455 3035 3818 3845 3921 4094 4202 Size: 872 192 580 783 27 76 173 108 Cuts at: 4202 1391 Size: 4072 Fragments arranged by size: 4072 872 783 580 192 173 108 76 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2299 3299 3893 928 Size: 971 400 1000 594 3918 Fragments arranged by size: 3918 1000 971 594 400 BbvCI CC'TCA_GC Cuts at: 3989 3989 Size: 6883 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2694 2728 3059 3093 5074 5108 1903 Size: 34 757 34 331 34 1981 34 3678 Fragments arranged by size: 3678 1981 757 331 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3628 4775 5985 3628 Size: 1147 1210 4526 Fragments arranged by size: 4526 1210 1147 BclI T'GATC_A Cuts at: 1548 2511 2559 1548 Size: 963 48 5872 Fragments arranged by size: 5872 963 48 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5494 136 Size: 107 122 71 631 605 3822 1525 Fragments arranged by size: 3822 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2919 6878 2919 Size: 3959 2924 BlpI GC'TnA_GC Cuts at: 4300 4300 Size: 6883 Bme1580I G_kGCm'C Cuts at: 1790 2263 2455 3247 3255 3666 4944 6100 1790 Size: 473 192 792 8 411 1278 1156 2573 Fragments arranged by size: 2573 1278 1156 792 473 411 192 8 BmgBI CAC'GTC Cuts at: 1318 2668 1318 Size: 1350 5533 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2606 4614 5572 Size: 416 53 83 399 24 1189 2008 958 Cuts at: 5572 442 Size: 1753 Fragments arranged by size: 2008 1753 1189 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6883 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2050 3049 3793 2050 Size: 999 744 5140 Fragments arranged by size: 5140 999 744 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3020 3512 3789 5543 1239 Size: 1781 492 277 1754 2579 Fragments arranged by size: 2579 1781 1754 492 277 Bpu10I CC'TnA_GC Cuts at: 3989 4001 3989 Size: 12 6871 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5008 5873 6171 5008 Size: 865 298 5720 Fragments arranged by size: 5720 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5546 882 Size: 617 4047 2219 Fragments arranged by size: 4047 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3150 3387 4674 493 Size: 711 1946 237 1287 2702 Fragments arranged by size: 2702 1946 1287 711 237 BsaBI GATnn'nnATC Cuts at: 3437 6877 3437 Size: 3440 3443 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2342 4690 5072 5707 Size: 53 83 186 936 809 2348 382 635 Cuts at: 5707 275 Size: 1451 Fragments arranged by size: 2348 1451 936 809 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5316 5714 5988 6135 6546 5316 Size: 398 274 147 411 5653 Fragments arranged by size: 5653 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 2571 2601 3080 Size: 30 165 30 828 30 310 30 479 Cuts at: 3080 3110 1178 Size: 30 4951 Fragments arranged by size: 4951 828 479 310 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2236 2918 3248 3370 3668 3716 3977 Size: 1027 23 682 330 122 298 48 261 Cuts at: 3977 3980 1186 Size: 3 4089 Fragments arranged by size: 4089 1027 682 330 298 261 122 48 23 3 BseYI C'CCAG_C Cuts at: 2448 2454 3082 3555 3817 3930 6086 6582 2448 Size: 6 628 473 262 113 2156 496 2749 Fragments arranged by size: 2749 2156 628 496 473 262 113 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2621 2672 3059 3777 3830 1163 Size: 573 885 51 387 718 53 4216 Fragments arranged by size: 4216 885 718 573 387 53 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2815 4333 5094 6122 1540 Size: 275 186 814 1518 761 1028 2301 Fragments arranged by size: 2301 1518 1028 814 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3056 4944 5029 6100 3056 Size: 1888 85 1071 3839 Fragments arranged by size: 3839 1888 1071 85 BsmI GAATG_Cn' Cuts at: 4394 4487 4394 Size: 93 6790 BsmBI CGTCTCn'nnnn_ Cuts at: 2009 3621 3650 2009 Size: 1612 29 5242 Fragments arranged by size: 5242 1612 29 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 2455 3035 3056 3247 3255 3666 Size: 399 473 192 580 21 191 8 411 Cuts at: 3666 3818 3845 3921 4094 4202 4944 5029 6100 Size: 152 27 76 173 108 742 85 1071 Cuts at: 6100 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 580 473 411 399 192 191 173 152 108 85 76 27 21 8 BspEI T'CCGG_A Cuts at: 6546 6546 Size: 6883 BspHI T'CATG_A Cuts at: 3108 4772 6502 3108 Size: 1664 1730 3489 Fragments arranged by size: 3489 1730 1664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 2694 2832 2855 2937 3333 3790 3805 Size: 1289 561 138 23 82 396 457 15 Cuts at: 3805 4238 844 Size: 433 3489 Fragments arranged by size: 3489 1289 561 457 433 396 138 82 23 15 BsrBI CCG'CTC Cuts at: 1394 1823 2395 4770 1394 Size: 429 572 2375 3507 Fragments arranged by size: 3507 2375 572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3573 5378 5552 65 Size: 1183 2325 1805 174 1396 Fragments arranged by size: 2325 1805 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2695 3015 5527 5714 1700 Size: 110 885 320 2512 187 2869 Fragments arranged by size: 2869 2512 885 320 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6883 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6883 BssSI C'ACGA_G Cuts at: 4943 5955 4943 Size: 1012 5871 BstAPI GCAn_nnn'nTGC Cuts at: 794 2604 3791 794 Size: 1810 1187 3886 Fragments arranged by size: 3886 1810 1187 BstBI TT'CG_AA Cuts at: 5687 5687 Size: 6883 BstEII G'GTnAC_C Cuts at: 1184 4063 1184 Size: 2879 4004 BstYI r'GATC_y Cuts at: 1972 2853 2919 4213 4580 4965 4982 6434 6520 Size: 881 66 1294 367 385 17 1452 86 Cuts at: 6520 6532 6878 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1294 881 385 367 346 86 66 17 12 Bsu36I CC'TnA_GG Cuts at: 2520 2520 Size: 6883 BtgI C'CryG_G Cuts at: 513 1065 2872 3210 513 Size: 552 1807 338 4186 Fragments arranged by size: 4186 1807 552 338 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6144 Fragments arranged by size: 6144 683 56 BtsI GCAGTG_nn' Cuts at: 2425 2542 3047 3427 4399 5193 5213 2425 Size: 117 505 380 972 794 20 4095 Fragments arranged by size: 4095 972 794 505 380 117 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6883 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6848 DraI TTT'AAA Cuts at: 4187 4187 Size: 6883 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2471 5890 809 Size: 507 1155 3419 1802 Fragments arranged by size: 3419 1802 1155 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 2382 2643 Size: 54 1425 50 275 18 456 96 261 Cuts at: 2643 2697 2715 3339 4330 5219 6848 8 Size: 54 18 624 991 889 1629 43 Fragments arranged by size: 1629 1425 991 889 624 456 275 261 96 54 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4330 1537 Size: 275 2518 4090 Fragments arranged by size: 4090 2518 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2007 3068 3578 3762 4813 1353 Size: 654 1061 510 184 1051 3423 Fragments arranged by size: 3423 1061 1051 654 510 184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2135 5470 5844 5990 387 Size: 1064 138 546 3335 374 146 1280 Fragments arranged by size: 3335 1280 1064 546 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3020 3512 3658 3789 4946 5543 6330 Size: 393 1388 492 146 131 1157 597 787 Cuts at: 6330 6598 1239 Size: 268 1524 Fragments arranged by size: 1524 1388 1157 787 597 492 393 268 146 131 EcoNI CCTnn'n_nnAGG Cuts at: 2129 2191 2216 2561 3002 2129 Size: 62 25 345 441 6010 Fragments arranged by size: 6010 441 345 62 25 EcoO109I rG'GnC_Cy Cuts at: 2260 2728 3103 3545 2260 Size: 468 375 442 5598 Fragments arranged by size: 5598 468 442 375 EcoRI G'AATT_C Cuts at: 4192 4570 4192 Size: 378 6505 FspI TGC'GCA Cuts at: 5389 5389 Size: 6883 HaeII r_GCGC'y Cuts at: 1678 6030 1678 Size: 4352 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 3521 3553 5524 Size: 32 135 32 488 32 1429 32 1971 Cuts at: 5524 5556 5598 5630 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1971 1429 488 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3952 4227 4473 669 Size: 988 281 2014 275 246 3079 Fragments arranged by size: 3079 2014 988 281 275 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6883 HpaI GTT'AAC Cuts at: 4473 4473 Size: 6883 KpnI G_GTAC'C Cuts at: 4208 4208 Size: 6883 MfeI C'AATT_G Cuts at: 4482 4482 Size: 6883 MluI A'CGCG_T Cuts at: 6759 6759 Size: 6883 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 3678 5997 6181 1305 Size: 969 1404 2319 184 2007 Fragments arranged by size: 2319 2007 1404 969 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2288 2384 10 Size: 54 1425 343 456 96 4509 Fragments arranged by size: 4509 1425 456 343 96 54 MslI CAynn'nnrTG Cuts at: 518 1166 3113 3189 3389 4841 5200 5359 518 Size: 648 1947 76 200 1452 359 159 2042 Fragments arranged by size: 2042 1947 1452 648 359 200 159 76 MspA1I CmG'CkG Cuts at: 1606 2366 2458 2604 2708 3300 3542 3613 4071 Size: 760 92 146 104 592 242 71 458 Cuts at: 4071 4299 4976 6124 6369 1606 Size: 228 677 1148 245 2120 Fragments arranged by size: 2120 1148 760 677 592 458 245 242 228 146 104 92 71 NcoI C'CATG_G Cuts at: 513 1065 2872 513 Size: 552 1807 4524 Fragments arranged by size: 4524 1807 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6883 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6883 NotI GC'GGCC_GC Cuts at: 4330 4330 Size: 6883 NspI r_CATG'y Cuts at: 1132 1229 2101 2943 3272 3430 4241 5786 1132 Size: 97 872 842 329 158 811 1545 2229 Fragments arranged by size: 2229 1545 872 842 811 329 158 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6883 PciI A'CATG_T Cuts at: 5782 5782 Size: 6883 PflMI CCAn_nnn'nTGG Cuts at: 1108 2256 2724 2878 3028 3562 3697 1108 Size: 1148 468 154 150 534 135 4294 Fragments arranged by size: 4294 1148 534 468 154 150 135 PfoI T'CCnGG_A Cuts at: 2217 2433 2997 2217 Size: 216 564 6103 Fragments arranged by size: 6103 564 216 PmeI GTTT'AAAC Cuts at: 4187 4187 Size: 6883 PmlI CAC'GTG Cuts at: 3387 3387 Size: 6883 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2231 2263 4952 4984 6465 6497 2231 Size: 32 2689 32 1481 32 2617 Fragments arranged by size: 2689 2617 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6883 PsiI TTA'TAA Cuts at: 4453 4453 Size: 6883 PspOMI G'GGCC_C Cuts at: 2259 2451 2259 Size: 192 6691 PstI C_TGCA'G Cuts at: 830 2181 2829 2934 4235 830 Size: 1351 648 105 1301 3478 Fragments arranged by size: 3478 1351 1301 648 105 PvuI CG_AT'CG Cuts at: 2001 2815 2001 Size: 814 6069 PvuII CAG'CTG Cuts at: 2366 2458 2604 3613 2366 Size: 92 146 1009 5636 Fragments arranged by size: 5636 1009 146 92 SalI G'TCGA_C Cuts at: 1936 4225 1936 Size: 2289 4594 SapI GCTCTTCn'nnn_ Cuts at: 3578 3762 3578 Size: 184 6699 SbfI CC_TGCA'GG Cuts at: 2934 4235 2934 Size: 1301 5582 ScaI AGT'ACT Cuts at: 3489 5131 6843 3489 Size: 1642 1712 3529 Fragments arranged by size: 3529 1712 1642 SfcI C'TryA_G Cuts at: 826 1045 2177 2825 2930 3133 3600 4231 5366 Size: 219 1132 648 105 203 467 631 1135 Cuts at: 5366 6047 6238 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1132 681 648 631 467 219 203 191 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6883 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6883 SmaI CCC'GGG Cuts at: 1872 4210 1872 Size: 2338 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4987 5888 6150 820 Size: 197 926 3044 901 262 1553 Fragments arranged by size: 3044 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4674 493 Size: 4181 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6883 SphI G_CATG'C Cuts at: 1132 3272 3430 4241 1132 Size: 2140 158 811 3774 Fragments arranged by size: 3774 2140 811 158 SspI AAT'ATT Cuts at: 5 52 4807 5 Size: 47 4755 2081 Fragments arranged by size: 4755 2081 47 StuI AGG'CCT Cuts at: 1517 2211 3999 1517 Size: 694 1788 4401 Fragments arranged by size: 4401 1788 694 StyI C'CwwG_G Cuts at: 513 1065 2836 2872 3621 4322 513 Size: 552 1771 36 749 701 3074 Fragments arranged by size: 3074 1771 749 701 552 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2879 4051 4910 4927 5080 5265 5710 Size: 1255 1299 1172 859 17 153 185 445 Cuts at: 5710 325 Size: 1498 Fragments arranged by size: 1498 1299 1255 1172 859 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 3487 3705 Size: 274 80 33 51 1443 117 1393 218 Cuts at: 3705 5129 6841 96 Size: 1424 1712 138 Fragments arranged by size: 1712 1443 1424 1393 274 218 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2571 2603 6843 6875 2571 Size: 32 4240 32 2579 Fragments arranged by size: 4240 2579 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6883 XbaI T'CTAG_A Cuts at: 4219 4219 Size: 6883 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6883 XmaI C'CCGG_G Cuts at: 1870 4208 1870 Size: 2338 4545 XmnI GAAnn'nnTTC Cuts at: 5012 5012 Size: 6883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4691 276 Size: 53 83 186 936 3157 2468 Fragments arranged by size: 3157 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI