Restriction map for
pFN21AE2067
(Linear) (Six-base) MAPSORT of: KIEE2067.seq Check: 9078 from: 1 to: 2178
KIEE2067.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 689 850 932 1785 1800 2178
Size: 689 161 82 853 15 378
Fragments arranged by size:
853 689 378 161 82 15
AccI GT'mk_AC
Cuts at: 0 419 1594 2178
Size: 419 1175 584
Fragments arranged by size:
1175 584 419
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1653 2178
Size: 1653 525
AflIII A'CryG_T
Cuts at: 0 1142 2178
Size: 1142 1036
AleI CACnn'nnGTG
Cuts at: 0 1184 2178
Size: 1184 994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1438 2178
Size: 1438 740
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 226 258 2178
Size: 226 32 1920
Fragments arranged by size:
1920 226 32
AlwNI CAG_nnn'CTG
Cuts at: 0 599 1557 2178
Size: 599 958 621
Fragments arranged by size:
958 621 599
ApaI G_GGCC'C
Cuts at: 0 258 450 2178
Size: 258 192 1728
Fragments arranged by size:
1728 258 192
ApoI r'AATT_y
Cuts at: 0 2122 2178
Size: 2122 56
AvaI C'yCGr_G
Cuts at: 0 19 85 2178
Size: 19 66 2093
Fragments arranged by size:
2093 66 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 934 967 2178
Size: 934 33 1211
Fragments arranged by size:
1211 934 33
BanI G'GyrC_C
Cuts at: 0 1237 1245 1656 2178
Size: 1237 8 411 522
Fragments arranged by size:
1237 522 411 8
BanII G_rGCy'C
Cuts at: 0 258 450 1030 1813 1840 1916 2089 2178
Size: 258 192 580 783 27 76 173 89
Fragments arranged by size:
783 580 258 192 173 89 76 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 1294 1888 2178
Size: 294 1000 594 290
Fragments arranged by size:
1000 594 294 290
BbvCI CC'TCA_GC
Cuts at: 0 1984 2178
Size: 1984 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 689 723 1054 1088 2178
Size: 689 34 331 34 1090
Fragments arranged by size:
1090 689 331 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1623 2178
Size: 1623 555
BclI T'GATC_A
Cuts at: 0 506 554 2178
Size: 506 48 1624
Fragments arranged by size:
1624 506 48
BglII A'GATC_T
Cuts at: 0 914 2178
Size: 914 1264
Bme1580I G_kGCm'C
Cuts at: 0 258 450 1242 1250 1661 2178
Size: 258 192 792 8 411 517
Fragments arranged by size:
792 517 411 258 192 8
BmgBI CAC'GTC
Cuts at: 0 663 2178
Size: 663 1515
BmrI ACTGGGnnnn_n'
Cuts at: 0 601 2178
Size: 601 1577
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 45 1044 1788 2178
Size: 45 999 744 390
Fragments arranged by size:
999 744 390 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 1507 1784 2178
Size: 1015 492 277 394
Fragments arranged by size:
1015 492 394 277
Bpu10I CC'TnA_GC
Cuts at: 0 1984 1996 2178
Size: 1984 12 182
Fragments arranged by size:
1984 182 12
BsaAI yAC'GTr
Cuts at: 0 1145 1382 2178
Size: 1145 237 796
Fragments arranged by size:
1145 796 237
BsaBI GATnn'nnATC
Cuts at: 0 1432 2178
Size: 1432 746
BsaHI Gr'CG_yC
Cuts at: 0 337 2178
Size: 337 1841
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 566 596 1075 1105 2178
Size: 226 30 310 30 479 30 1073
Fragments arranged by size:
1073 479 310 226 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 231 913 1243 1365 1663 1711 1972
Size: 208 23 682 330 122 298 48 261
Cuts at: 1972 1975 2178
Size: 3 203
Fragments arranged by size:
682 330 298 261 208 203 122 48
23 3
BseYI C'CCAG_C
Cuts at: 0 443 449 1077 1550 1812 1925 2178
Size: 443 6 628 473 262 113 253
Fragments arranged by size:
628 473 443 262 253 113 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 667 1054 1772 1825 2178
Size: 616 51 387 718 53 353
Fragments arranged by size:
718 616 387 353 53 51
BsiEI CG_ry'CG
Cuts at: 0 810 2178
Size: 810 1368
BsiHKAI G_wGCw'C
Cuts at: 0 1051 2178
Size: 1051 1127
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4 1616 1645 2178
Size: 4 1612 29 533
Fragments arranged by size:
1612 533 29 4
Bsp1286I G_dGCh'C
Cuts at: 0 258 450 1030 1051 1242 1250 1661 1813
Size: 258 192 580 21 191 8 411 152
Cuts at: 1813 1840 1916 2089 2178
Size: 27 76 173 89
Fragments arranged by size:
580 411 258 192 191 173 152 89
76 27 21 8
BspHI T'CATG_A
Cuts at: 0 1103 2178
Size: 1103 1075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 689 827 850 932 1328 1785 1800
Size: 128 561 138 23 82 396 457 15
Cuts at: 1800 2178
Size: 378
Fragments arranged by size:
561 457 396 378 138 128 82 23
15
BsrBI CCG'CTC
Cuts at: 0 390 2178
Size: 390 1788
BsrDI GCAATG_nn'
Cuts at: 0 1568 2178
Size: 1568 610
BsrFI r'CCGG_y
Cuts at: 0 690 1010 2178
Size: 690 320 1168
Fragments arranged by size:
1168 690 320
BstAPI GCAn_nnn'nTGC
Cuts at: 0 599 1786 2178
Size: 599 1187 392
Fragments arranged by size:
1187 599 392
BstEII G'GTnAC_C
Cuts at: 0 2058 2178
Size: 2058 120
BstYI r'GATC_y
Cuts at: 0 848 914 2178
Size: 848 66 1264
Fragments arranged by size:
1264 848 66
Bsu36I CC'TnA_GG
Cuts at: 0 515 2178
Size: 515 1663
BtgI C'CryG_G
Cuts at: 0 867 1205 2178
Size: 867 338 973
Fragments arranged by size:
973 867 338
BtsI GCAGTG_nn'
Cuts at: 0 420 537 1042 1422 2178
Size: 420 117 505 380 756
Fragments arranged by size:
756 505 420 380 117
DrdI GACnn_nn'nnGTC
Cuts at: 0 466 2178
Size: 466 1712
EaeI y'GGCC_r
Cuts at: 0 281 377 638 692 710 1334 2178
Size: 281 96 261 54 18 624 844
Fragments arranged by size:
844 624 281 261 96 54 18
EarI CTCTTCn'nnn_
Cuts at: 0 2 1063 1573 1757 2178
Size: 2 1061 510 184 421
Fragments arranged by size:
1061 510 421 184 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 130 2178
Size: 130 2048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 1507 1653 1784 2178
Size: 1015 492 146 131 394
Fragments arranged by size:
1015 492 394 146 131
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 186 211 556 997 2178
Size: 124 62 25 345 441 1181
Fragments arranged by size:
1181 441 345 124 62 25
EcoO109I rG'GnC_Cy
Cuts at: 0 255 723 1098 1540 2178
Size: 255 468 375 442 638
Fragments arranged by size:
638 468 442 375 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 1516 1548 2178
Size: 55 32 1429 32 630
Fragments arranged by size:
1429 630 55 32 32
HincII GTy'rAC
Cuts at: 0 1947 2178
Size: 1947 231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1673 2178
Size: 269 1404 505
Fragments arranged by size:
1404 505 269
MscI TGG'CCA
Cuts at: 0 283 379 2178
Size: 283 96 1799
Fragments arranged by size:
1799 283 96
MslI CAynn'nnrTG
Cuts at: 0 1108 1184 1384 2178
Size: 1108 76 200 794
Fragments arranged by size:
1108 794 200 76
MspA1I CmG'CkG
Cuts at: 0 361 453 599 703 1295 1537 1608 2066
Size: 361 92 146 104 592 242 71 458
Cuts at: 2066 2178
Size: 112
Fragments arranged by size:
592 458 361 242 146 112 104 92
71
NcoI C'CATG_G
Cuts at: 0 867 2178
Size: 867 1311
NspI r_CATG'y
Cuts at: 0 96 938 1267 1425 2178
Size: 96 842 329 158 753
Fragments arranged by size:
842 753 329 158 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 251 719 873 1023 1557 1692 2178
Size: 251 468 154 150 534 135 486
Fragments arranged by size:
534 486 468 251 154 150 135
PfoI T'CCnGG_A
Cuts at: 0 212 428 992 2178
Size: 212 216 564 1186
Fragments arranged by size:
1186 564 216 212
PmlI CAC'GTG
Cuts at: 0 1382 2178
Size: 1382 796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 2178
Size: 226 32 1920
Fragments arranged by size:
1920 226 32
PspOMI G'GGCC_C
Cuts at: 0 254 446 2178
Size: 254 192 1732
Fragments arranged by size:
1732 254 192
PstI C_TGCA'G
Cuts at: 0 176 824 929 2178
Size: 176 648 105 1249
Fragments arranged by size:
1249 648 176 105
PvuI CG_AT'CG
Cuts at: 0 810 2178
Size: 810 1368
PvuII CAG'CTG
Cuts at: 0 361 453 599 1608 2178
Size: 361 92 146 1009 570
Fragments arranged by size:
1009 570 361 146 92
SapI GCTCTTCn'nnn_
Cuts at: 0 1573 1757 2178
Size: 1573 184 421
Fragments arranged by size:
1573 421 184
SbfI CC_TGCA'GG
Cuts at: 0 929 2178
Size: 929 1249
ScaI AGT'ACT
Cuts at: 0 1484 2178
Size: 1484 694
SfcI C'TryA_G
Cuts at: 0 172 820 925 1128 1595 2178
Size: 172 648 105 203 467 583
Fragments arranged by size:
648 583 467 203 172 105
SphI G_CATG'C
Cuts at: 0 1267 1425 2178
Size: 1267 158 753
Fragments arranged by size:
1267 753 158
StuI AGG'CCT
Cuts at: 0 206 1994 2178
Size: 206 1788 184
Fragments arranged by size:
1788 206 184
StyI C'CwwG_G
Cuts at: 0 831 867 1616 2178
Size: 831 36 749 562
Fragments arranged by size:
831 749 562 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 874 2046 2178
Size: 874 1172 132
Fragments arranged by size:
1172 874 132
TatI w'GTAC_w
Cuts at: 0 89 1482 1700 2178
Size: 89 1393 218 478
Fragments arranged by size:
1393 478 218 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 566 598 2178
Size: 566 32 1580
Fragments arranged by size:
1580 566 32
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApoI AvaI BaeI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstEII BstYI Bsu36I BtgI BtsI
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I
HincII MmeI MscI MslI MspA1I NcoI NspI PflMI
PfoI PmlI PpiI PspOMI PstI PvuI PvuII SapI
SbfI ScaI SfcI SphI StuI StyI TaqII TatI
TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI ApaLI
AscI AseI AsiSI AvrII BamHI BbeI BfrBI BglI
BlpI BmtI BpuEI BsaI BsaWI BsiWI BsmI BspEI
BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PpuMI PshAI
PsiI PspXI PsrI RsrII SacI SacII SalI SanDI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI