Restriction map for
pFN21AE1565
##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccII AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsiEI BsiWI Bsp1407I BspDI BsrBI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NarI NcoI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 1619 TCCGGA T^CCGGA
AclI 1552 AACGTT AA^CGTT
AseI 1389 ATTAAT AT^TAAT
AsnI 1389 ATTAAT AT^TAAT
Asp700I 2034 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 110 CCCGGG C^[CT]CG[GA]G
BamHI 832 GGATCC G^GATCC
BanI 105 GGTGCC G^G[CT][GA]CC
BbsI 444 GTCTTC GAAGAC(2/6)
BfaI 1483 CTAG C^TAG
BpuAI 444 GTCTTC GAAGAC(2/6)
BsaAI 540 CACGTA [CT]AC^GT[GA]
BsaBI 1896 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1619 TCCGGA T^CCGGA
BseRI 1412 GAGGAG GAGGAG(10/8)
BsmBI 1714 GAGACG CGTCTC(1/5)
BsmI 801 GAATGC GAATGC(1/-1)
BsoBI 110 CCCGGG C^[CT]CG[GA]G
BspEI 1619 TCCGGA T^CCGGA
BspLU11I 2148 ACATGT A^CATGT
BsrDI 793 GCAATG GCAATG(2/0)
BssSI 144 CACGAG CACGAG(-5/-1)
BstBI 512 TTCGAA TT^CGAA
BstXI 868 CCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1968 TTTAAA TTT^AAA
DraII 1688 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 814 GACGAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1911 CCGTGG C^C[GA][CT]GG
EcoNI 156 CCTGTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1688 GGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 1234 CCCGC CCCGC(4/6)
MaeI 1483 CTAG C^TAG
MamI 1896 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1619 TCCGGA T^CCGGA
NdeI 275 CATATG CA^TATG
NspV 512 TTCGAA TT^CGAA
PflMI 668 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1688 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 262 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1389 ATTAAT AT^TAAT
Psp1406I 1552 AACGTT AA^CGTT
PvuII 1792 CAGCTG CAG^CTG
SacI 912 GAGCTC GAGCT^C
SapI 35 GAAGAGC GCTCTTC(1/4)
SfcI 362 CTATAG C^T[GA][CT]AG
SfuI 512 TTCGAA TT^CGAA
SmaI 110 CCCGGG CCC^GGG
StuI 1147 AGGCCT AGG^CCT
Van91I 668 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 110 CCCGGG C^CCGGG
XmaI 110 CCCGGG C^CCGGG
XmnI 2034 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 115 GGCGTC G[GA]^CG[CT]C
859 GGCGTC
AflIII 539 ACACGT A^C[GA][CT]GT
2148 ACATGT
AlwNI 669 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1792 CAGCTGCTG
AspHI 734 GAGCAC G[AT]GC[AT]^C
912 GAGCTC
BalI 936 TGGCCA TGG^CCA
1859 TGGCCA
BanII 912 GAGCTC G[GA]GC[CT]^C
1735 GAGCCC
BbvI 64 GCAGC GCAGC(8/12)
1794 GCTGC
BsaHI 115 GGCGTC G[GA]^CG[CT]C
859 GGCGTC
BsaI 1843 GAGACC GGTCTC(1/5)
2097 GGTCTC
BsiHKAI 734 GAGCAC G[AT]GC[AT]^C
912 GAGCTC
BspHI 407 TCATGA T^CATGA
1535 TCATGA
BspMI 77 ACCTGC ACCTGC(4/8)
122 ACCTGC
BsrFI 1774 GCCGGC [GA]^CCGG[CT]
2087 GCCGGC
BstYI 291 GGATCT [GA]^GATC[CT]
832 GGATCC
Cfr10I 1774 GCCGGC [GA]^CCGG[CT]
2087 GCCGGC
EcoRI 1083 GAATTC G^AATTC
1702 GAATTC
Hin1I 115 GGCGTC G[GA]^CG[CT]C
859 GGCGTC
MflI 291 GGATCT [GA]^GATC[CT]
832 GGATCC
MluNI 936 TGGCCA TGG^CCA
1859 TGGCCA
MscI 936 TGGCCA TGG^CCA
1859 TGGCCA
MspA1I 65 CAGCGG C[AC]G^C[GT]G
1792 CAGCTG
NaeI 1774 GCCGGC GCC^GGC
2087 GCCGGC
NgoMIV 1774 GCCGGC G^CCGGC
2087 GCCGGC
PleI 495 GACTC GAGTC(4/5)
2137 GACTC
RcaI 407 TCATGA T^CATGA
1535 TCATGA
TseI 64 GCAGC G^C[AT]GC
1794 GCTGC
XhoII 291 GGATCT [GA]^GATC[CT]
832 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BcnI BmyI BpmI
BsaJI BsaWI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI TthHB8I
XcmI