(Circular) (Six-base) MAPSORT of: pFN21AE1565.seq Check: 8690 from: 1 to: 6901 pFN21AE1565 6901 bp With 182 enzymes: SgfI * January 20, 2017 10:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4711 278 Size: 53 83 186 936 3175 2468 Fragments arranged by size: 3175 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4244 1937 Size: 2307 4594 Acc65I G'GTAC_C Cuts at: 4222 4222 Size: 6901 AclI AA'CG_TT Cuts at: 1563 3558 5028 5401 1563 Size: 1995 1470 373 3063 Fragments arranged by size: 3063 1995 1470 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2254 2287 4964 6348 6616 1632 Size: 452 170 33 2677 1384 268 1917 Fragments arranged by size: 2677 1917 1384 452 268 170 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6704 AflIII A'CryG_T Cuts at: 1203 2544 4153 5800 6777 1203 Size: 1341 1609 1647 977 1327 Fragments arranged by size: 1647 1609 1341 1327 977 AgeI A'CCGG_T Cuts at: 5732 5732 Size: 6901 AhdI GACnn_n'nnGTC Cuts at: 5630 5630 Size: 6901 AleI CACnn'nnGTG Cuts at: 1166 3356 1166 Size: 2190 4711 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6901 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3074 3106 3074 Size: 32 6869 AlwNI CAG_nnn'CTG Cuts at: 2679 3802 4644 6216 2679 Size: 1123 842 1572 3364 Fragments arranged by size: 3364 1572 1123 842 ApaLI G'TGCA_C Cuts at: 4958 6114 4958 Size: 1156 5745 ApoI r'AATT_y Cuts at: 1493 2474 3088 3452 3707 4210 4436 4588 6697 Size: 981 614 364 255 503 226 152 2109 Cuts at: 6697 1493 Size: 1697 Fragments arranged by size: 2109 1697 981 614 503 364 255 226 152 AseI AT'TA_AT Cuts at: 160 1032 3395 5455 160 Size: 872 2363 2060 1606 Fragments arranged by size: 2363 2060 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 AvaI C'yCGr_G Cuts at: 1870 1943 2115 4226 1870 Size: 73 172 2111 4545 Fragments arranged by size: 4545 2111 172 73 BamHI G'GATC_C Cuts at: 2837 4231 4598 2837 Size: 1394 367 5140 Fragments arranged by size: 5140 1394 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 4222 5677 618 Size: 325 217 627 323 2112 1455 1842 Fragments arranged by size: 2112 1842 1455 627 325 323 217 BanII G_rGCy'C Cuts at: 1391 2921 3744 4220 1391 Size: 1530 823 476 4072 Fragments arranged by size: 4072 1530 823 476 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2442 928 Size: 971 543 5387 Fragments arranged by size: 5387 971 543 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5092 5126 1903 Size: 34 3155 34 3678 Fragments arranged by size: 3678 3155 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4793 6003 4793 Size: 1210 5691 BclI T'GATC_A Cuts at: 1548 1548 Size: 6901 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5512 136 Size: 107 122 71 631 605 3840 1525 Fragments arranged by size: 3840 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6896 6896 Size: 6901 BlpI GC'TnA_GC Cuts at: 4318 4318 Size: 6901 Bme1580I G_kGCm'C Cuts at: 1790 2115 4962 6118 1790 Size: 325 2847 1156 2573 Fragments arranged by size: 2847 2573 1156 325 BmgBI CAC'GTC Cuts at: 1318 3730 4174 1318 Size: 2412 444 4045 Fragments arranged by size: 4045 2412 444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2354 4632 5590 Size: 416 53 83 399 24 937 2278 958 Cuts at: 5590 442 Size: 1753 Fragments arranged by size: 2278 1753 958 937 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6901 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2701 3758 4038 5561 1239 Size: 1462 1057 280 1523 2579 Fragments arranged by size: 2579 1523 1462 1057 280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5026 5891 6189 5026 Size: 865 298 5738 Fragments arranged by size: 5738 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3842 4108 5564 882 Size: 617 2343 266 1456 2219 Fragments arranged by size: 2343 2219 1456 617 266 BsaAI yAC'GTr Cuts at: 493 1204 2547 4692 493 Size: 711 1343 2145 2702 Fragments arranged by size: 2702 2145 1343 711 BsaBI GATnn'nnATC Cuts at: 3905 6895 3905 Size: 2990 3911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 2865 4708 5090 Size: 53 83 186 936 588 744 1843 382 Cuts at: 5090 5725 275 Size: 635 1451 Fragments arranged by size: 1843 1451 936 744 635 588 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2913 2934 3624 5334 5732 6006 6153 6564 2913 Size: 21 690 1710 398 274 147 411 3250 Fragments arranged by size: 3250 1710 690 411 398 274 147 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2674 2704 3731 3761 1178 Size: 30 165 30 1271 30 1027 30 4318 Fragments arranged by size: 4318 1271 1027 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3432 1186 Size: 2246 4655 BseYI C'CCAG_C Cuts at: 2949 3592 6104 6600 2949 Size: 643 2512 496 3250 Fragments arranged by size: 3250 2512 643 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 3803 4094 4196 1163 Size: 573 543 1524 291 102 3868 Fragments arranged by size: 3868 1524 573 543 291 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4351 5112 6140 1540 Size: 275 186 2350 761 1028 2301 Fragments arranged by size: 2350 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2743 2921 4962 5047 6118 2743 Size: 178 2041 85 1071 3526 Fragments arranged by size: 3526 2041 1071 178 85 BsmI GAATG_Cn' Cuts at: 2812 4412 4505 2812 Size: 1600 93 5208 Fragments arranged by size: 5208 1600 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3713 3713 Size: 6901 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2743 2921 3744 4220 4962 5047 Size: 399 325 628 178 823 476 742 85 Cuts at: 5047 6118 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 823 742 628 476 399 325 178 85 BspEI T'CCGG_A Cuts at: 3624 6564 3624 Size: 2940 3961 BspHI T'CATG_A Cuts at: 2412 3540 4790 6520 2412 Size: 1128 1250 1730 2793 Fragments arranged by size: 2793 1730 1250 1128 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2091 2136 4256 844 Size: 1247 45 2120 3489 Fragments arranged by size: 3489 2120 1247 45 BsrBI CCG'CTC Cuts at: 1394 1823 4788 1394 Size: 429 2965 3507 Fragments arranged by size: 3507 2965 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2805 5396 5570 65 Size: 1183 1557 2591 174 1396 Fragments arranged by size: 2591 1557 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3779 4092 5545 5732 1700 Size: 110 1969 313 1453 187 2869 Fragments arranged by size: 2869 1969 1453 313 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6901 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6901 BssSI C'ACGA_G Cuts at: 2149 4961 5973 2149 Size: 2812 1012 3077 Fragments arranged by size: 3077 2812 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6901 BstBI TT'CG_AA Cuts at: 2518 5705 2518 Size: 3187 3714 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6901 BstXI CCAn_nnnn'nTGG Cuts at: 2880 2880 Size: 6901 BstYI r'GATC_y Cuts at: 1972 2296 2837 4231 4598 4983 5000 6452 6538 Size: 324 541 1394 367 385 17 1452 86 Cuts at: 6538 6550 6896 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1394 541 385 367 346 324 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3916 4197 513 Size: 552 2851 281 3217 Fragments arranged by size: 3217 2851 552 281 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3231 487 Size: 683 56 2005 4157 Fragments arranged by size: 4157 2005 683 56 BtsI GCAGTG_nn' Cuts at: 3124 4082 4144 4417 5211 5231 3124 Size: 958 62 273 794 20 4794 Fragments arranged by size: 4794 958 794 273 62 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6901 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3367 3402 3898 3933 529 Size: 35 2803 35 496 35 3497 Fragments arranged by size: 3497 2803 496 35 35 35 DraI TTT'AAA Cuts at: 3975 4205 3975 Size: 230 6671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2825 5908 809 Size: 507 1509 3083 1802 Fragments arranged by size: 3083 1802 1509 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2941 3265 3864 Size: 54 1425 50 275 18 1111 324 599 Cuts at: 3864 4348 5237 6866 8 Size: 484 889 1629 43 Fragments arranged by size: 1629 1425 1111 889 599 484 324 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4348 1537 Size: 275 2536 4090 Fragments arranged by size: 4090 2536 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2035 2306 2561 2607 2755 3468 4831 1353 Size: 682 271 255 46 148 713 1363 3423 Fragments arranged by size: 3423 1363 713 682 271 255 148 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5488 5862 6008 387 Size: 1064 138 3899 374 146 1280 Fragments arranged by size: 3899 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2919 2919 Size: 6901 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2254 2287 2701 3758 4038 4964 Size: 393 452 170 33 414 1057 280 926 Cuts at: 4964 5561 6348 6616 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1057 926 787 597 452 414 393 280 268 170 33 EcoNI CCTnn'n_nnAGG Cuts at: 2165 2165 Size: 6901 EcoO109I rG'GnC_Cy Cuts at: 3694 3694 Size: 6901 EcoRI G'AATT_C Cuts at: 3088 3707 4210 4588 3088 Size: 619 503 378 5401 Fragments arranged by size: 5401 619 503 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2156 4209 2156 Size: 2053 4848 FspI TGC'GCA Cuts at: 5407 5407 Size: 6901 HaeII r_GCGC'y Cuts at: 1678 6048 1678 Size: 4370 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2411 2443 2674 2706 3731 Size: 32 135 32 839 32 231 32 1025 Cuts at: 3731 3763 5542 5574 5616 5648 1373 Size: 32 1779 32 42 32 2626 Fragments arranged by size: 2626 1779 1025 839 231 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2125 2896 3313 3562 4245 4491 Size: 988 281 187 771 417 249 683 246 Cuts at: 4491 669 Size: 3079 Fragments arranged by size: 3079 988 771 683 417 281 249 246 187 HindIII A'AGCT_T Cuts at: 748 748 Size: 6901 HpaI GTT'AAC Cuts at: 4491 4491 Size: 6901 KpnI G_GTAC'C Cuts at: 4226 4226 Size: 6901 MfeI C'AATT_G Cuts at: 4500 4500 Size: 6901 MluI A'CGCG_T Cuts at: 6777 6777 Size: 6901 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2815 3148 3579 6015 6199 1305 Size: 1510 333 431 2436 184 2007 Fragments arranged by size: 2436 2007 1510 431 333 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2943 3866 10 Size: 54 1425 343 1111 923 3045 Fragments arranged by size: 3045 1425 1111 923 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 3036 3267 3356 3947 4008 4158 Size: 648 1194 676 231 89 591 61 150 Cuts at: 4158 4859 5218 5377 518 Size: 701 359 159 2042 Fragments arranged by size: 2042 1194 701 676 648 591 359 231 159 150 89 61 MspA1I CmG'CkG Cuts at: 1606 2072 3799 4317 4994 6142 6387 1606 Size: 466 1727 518 677 1148 245 2120 Fragments arranged by size: 2120 1727 1148 677 518 466 245 NaeI GCC'GGC Cuts at: 3781 4094 3781 Size: 313 6588 NcoI C'CATG_G Cuts at: 513 1065 4197 513 Size: 552 3132 3217 Fragments arranged by size: 3217 3132 552 NdeI CA'TA_TG Cuts at: 387 2281 387 Size: 1894 5007 NgoMIV G'CCGG_C Cuts at: 3779 4092 3779 Size: 313 6588 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6901 NotI GC'GGCC_GC Cuts at: 4348 4348 Size: 6901 NspI r_CATG'y Cuts at: 1132 1229 2485 3226 4157 4259 5804 1132 Size: 97 1256 741 931 102 1545 2229 Fragments arranged by size: 2229 1545 1256 931 741 102 97 PasI CC'CwG_GG Cuts at: 1348 4098 1348 Size: 2750 4151 PciI A'CATG_T Cuts at: 4153 5800 4153 Size: 1647 5254 PflMI CCAn_nnn'nTGG Cuts at: 1108 2679 1108 Size: 1571 5330 PfoI T'CCnGG_A Cuts at: 2018 2709 3066 4015 2018 Size: 691 357 949 4904 Fragments arranged by size: 4904 949 691 357 PmeI GTTT'AAAC Cuts at: 4205 4205 Size: 6901 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4970 5002 6483 6515 4970 Size: 32 1481 32 5356 Fragments arranged by size: 5356 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3694 3694 Size: 6901 PshAI GACnn'nnGTC Cuts at: 1613 2271 1613 Size: 658 6243 PsiI TTA'TAA Cuts at: 4471 4471 Size: 6901 PstI C_TGCA'G Cuts at: 830 4253 830 Size: 3423 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6901 PvuII CAG'CTG Cuts at: 3799 3799 Size: 6901 SacI G_AGCT'C Cuts at: 2921 2921 Size: 6901 SalI G'TCGA_C Cuts at: 1936 4243 1936 Size: 2307 4594 SanDI GG'GwC_CC Cuts at: 3694 3694 Size: 6901 SapI GCTCTTCn'nnn_ Cuts at: 2035 2035 Size: 6901 SbfI CC_TGCA'GG Cuts at: 4253 4253 Size: 6901 ScaI AGT'ACT Cuts at: 5149 6861 5149 Size: 1712 5189 SfcI C'TryA_G Cuts at: 826 1045 2367 4249 5384 6065 6256 826 Size: 219 1322 1882 1135 681 191 1471 Fragments arranged by size: 1882 1471 1322 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6901 SmaI CCC'GGG Cuts at: 1872 2117 4228 1872 Size: 245 2111 4545 Fragments arranged by size: 4545 2111 245 SmlI C'TyrA_G Cuts at: 820 1017 1943 5005 5906 6168 820 Size: 197 926 3062 901 262 1553 Fragments arranged by size: 3062 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4692 493 Size: 4199 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6901 SphI G_CATG'C Cuts at: 1132 4259 1132 Size: 3127 3774 SspI AAT'ATT Cuts at: 5 52 4825 5 Size: 47 4773 2081 Fragments arranged by size: 4773 2081 47 StuI AGG'CCT Cuts at: 1517 3154 1517 Size: 1637 5264 StyI C'CwwG_G Cuts at: 513 1065 2564 2989 3102 4197 4340 513 Size: 552 1499 425 113 1095 143 3074 Fragments arranged by size: 3074 1499 1095 552 425 143 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2068 3395 4928 4945 5098 5283 5728 Size: 1255 488 1327 1533 17 153 185 445 Cuts at: 5728 325 Size: 1498 Fragments arranged by size: 1533 1498 1327 1255 488 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3786 5147 6859 Size: 274 80 33 51 1443 1809 1361 1712 Cuts at: 6859 96 Size: 138 Fragments arranged by size: 1809 1712 1443 1361 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3098 3130 4073 4105 6861 6893 3098 Size: 32 943 32 2756 32 3106 Fragments arranged by size: 3106 2756 943 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6901 XbaI T'CTAG_A Cuts at: 4237 4237 Size: 6901 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2464 3052 3562 3859 2464 Size: 588 510 297 5506 Fragments arranged by size: 5506 588 510 297 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6901 XmaI C'CCGG_G Cuts at: 1870 2115 4226 1870 Size: 245 2111 4545 Fragments arranged by size: 4545 2111 245 XmnI GAAnn'nnTTC Cuts at: 4043 5030 4043 Size: 987 5914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4709 276 Size: 53 83 186 936 3175 2468 Fragments arranged by size: 3175 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI