################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MroI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 1074 GGTACC G^GTACC AcsI 1249 GAATTC [GA]^AATT[CT] AcyI 766 GACGCC G[GA]^CG[CT]C ApoI 1249 GAATTC [GA]^AATT[CT] Asp718I 1074 GGTACC G^GTACC AspHI 778 GAGCTC G[AT]GC[AT]^C AvaI 547 CTCGGG C^[CT]CG[GA]G BamHI 96 GGATCC G^GATCC BanI 1074 GGTACC G^G[CT][GA]CC BfaI 832 CTAG C^TAG BsaHI 766 GACGCC G[GA]^CG[CT]C BsaI 490 GAGACC GGTCTC(1/5) BsgI 1417 GTGCAG GTGCAG(16/14) BsiHKAI 778 GAGCTC G[AT]GC[AT]^C BsmI 711 GAATGC GAATGC(1/-1) BsoBI 547 CTCGGG C^[CT]CG[GA]G Bsp1407I 728 TGTACA T^GTACA BsrDI 475 GCAATG GCAATG(2/0) BsrFI 824 GCCGGC [GA]^CCGG[CT] BsrGI 728 TGTACA T^GTACA BstXI 1382 CCATCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 824 GCCGGC [GA]^CCGG[CT] EarI 1432 CTCTTC CTCTTC(1/4) EcoNI 531 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1249 GAATTC G^AATTC HapII 825 CCGG C^CGG HgaI 766 GACGC GACGC(5/10) Hin1I 766 GACGCC G[GA]^CG[CT]C HpaII 825 CCGG C^CGG KpnI 1074 GGTACC GGTAC^C Ksp632I 1432 CTCTTC CTCTTC(1/4) MaeI 832 CTAG C^TAG MseI 1274 TTAA T^TAA MspA1I 1036 CAGCTG C[AC]G^C[GT]G MspI 825 CCGG C^CGG NaeI 824 GCCGGC GCC^GGC NgoMIV 824 GCCGGC G^CCGGC PvuII 1036 CAGCTG CAG^CTG SacI 778 GAGCTC GAGCT^C SspBI 728 TGTACA T^GTACA TaqI 1256 TCGA T^CGA TfiI 334 GATTC G^A[AT]TC Tru9I 1274 TTAA T^TAA TthHB8I 1256 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Asp700I 630 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 931 GAACCCTTTC BalI 689 TGGCCA TGG^CCA 797 TGGCCA BanII 277 GAGCCC G[GA]GC[CT]^C 778 GAGCTC BglII 668 AGATCT A^GATCT 1302 AGATCT BseRI 60 CTCCTC GAGGAG(10/8) 865 CTCCTC BsmFI 698 GGGAC GGGAC(10/14) 1445 GGGAC EaeI 689 TGGCCA [CT]^GGCC[GA] 797 TGGCCA EcoT14I 599 CCAAGG C^C[AT][AT]GG 1025 CCAAGG MluNI 689 TGGCCA TGG^CCA 797 TGGCCA MscI 689 TGGCCA TGG^CCA 797 TGGCCA PleI 402 GAGTC GAGTC(4/5) 746 GAGTC PstI 1039 CTGCAG CTGCA^G 1370 CTGCAG SfcI 1039 CTGCAG C^T[GA][CT]AG 1370 CTGCAG StyI 599 CCAAGG C^C[AT][AT]GG 1025 CCAAGG XcmI 345 CCAGCCCACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 566 CCAAAACAAACCTGG XmnI 630 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 931 GAACCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BbsI BbvI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI XhoII