(Circular) (Six-base) MAPSORT of: pFN21AE1413.seq Check: 2636 from: 1 to: 6205 pFN21AE1413 6205 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3548 1937 Size: 1611 4594 Acc65I G'GTAC_C Cuts at: 3079 3526 3079 Size: 447 5758 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2477 2654 3296 4268 5652 5920 1632 Size: 845 177 642 972 1384 268 1917 Fragments arranged by size: 1917 1384 972 845 642 268 177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 2711 2798 1166 Size: 1545 87 4573 Fragments arranged by size: 4573 1545 87 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AlwNI CAG_nnn'CTG Cuts at: 2381 2548 2632 3091 3359 3948 5520 2381 Size: 167 84 459 268 589 1572 3066 Fragments arranged by size: 3066 1572 589 459 268 167 84 ApaLI G'TGCA_C Cuts at: 4262 5418 4262 Size: 1156 5049 ApoI r'AATT_y Cuts at: 1493 3254 3514 3740 3892 6001 1493 Size: 1761 260 226 152 2109 1697 Fragments arranged by size: 2109 1761 1697 260 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2552 3530 1870 Size: 73 609 978 4545 Fragments arranged by size: 4545 978 609 73 BamHI G'GATC_C Cuts at: 2101 3535 3902 2101 Size: 1434 367 4404 Fragments arranged by size: 4404 1434 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3079 3526 4981 618 Size: 325 217 627 1292 447 1455 1842 Fragments arranged by size: 1842 1455 1292 627 447 325 217 BanII G_rGCy'C Cuts at: 1391 2286 2787 3524 1391 Size: 895 501 737 4072 Fragments arranged by size: 4072 895 737 501 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2909 3114 3466 928 Size: 971 1010 205 352 3667 Fragments arranged by size: 3667 1010 971 352 205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4396 4430 1903 Size: 34 2459 34 3678 Fragments arranged by size: 3678 2459 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4816 136 Size: 107 122 71 631 605 3144 1525 Fragments arranged by size: 3144 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2673 3307 6200 2673 Size: 634 2893 2678 Fragments arranged by size: 2893 2678 634 BlpI GC'TnA_GC Cuts at: 3622 3622 Size: 6205 Bme1580I G_kGCm'C Cuts at: 1790 2973 4266 5422 1790 Size: 1183 1293 1156 2573 Fragments arranged by size: 2573 1293 1183 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2370 3369 3936 Size: 416 53 83 399 24 953 999 567 Cuts at: 3936 4894 442 Size: 958 1753 Fragments arranged by size: 1753 999 958 953 567 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1996 2074 2204 2603 3182 4865 1239 Size: 757 78 130 399 579 1683 2579 Fragments arranged by size: 2579 1683 757 579 399 130 78 Bpu10I CC'TnA_GC Cuts at: 2756 2756 Size: 6205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4330 5195 5493 4330 Size: 865 298 5042 Fragments arranged by size: 5042 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2489 4868 882 Size: 617 990 2379 2219 Fragments arranged by size: 2379 2219 990 617 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 6199 6199 Size: 6205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2772 4012 4394 5029 Size: 53 83 186 936 1239 1240 382 635 Cuts at: 5029 275 Size: 1451 Fragments arranged by size: 1451 1240 1239 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4638 5036 5310 5457 5868 4638 Size: 398 274 147 411 4975 Fragments arranged by size: 4975 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2326 2356 1178 Size: 30 165 30 923 30 5027 Fragments arranged by size: 5027 923 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2056 2861 1186 Size: 870 805 4530 Fragments arranged by size: 4530 870 805 BseYI C'CCAG_C Cuts at: 2051 2325 2349 2558 5408 5904 2051 Size: 274 24 209 2850 496 2352 Fragments arranged by size: 2850 2352 496 274 209 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3443 1163 Size: 573 1707 3925 Fragments arranged by size: 3925 1707 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2787 4266 4351 5422 2787 Size: 1479 85 1071 3570 Fragments arranged by size: 3570 1479 1071 85 BsmI GAATG_Cn' Cuts at: 2722 3716 3809 2722 Size: 994 93 5118 Fragments arranged by size: 5118 994 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 2787 2973 3524 4266 4351 5422 Size: 399 496 501 186 551 742 85 1071 Cuts at: 5422 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 551 501 496 399 186 85 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 4094 5824 4094 Size: 1730 4475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3560 844 Size: 2716 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2487 4700 4874 65 Size: 1183 1239 2213 174 1396 Fragments arranged by size: 2213 1396 1239 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2829 4849 5036 1700 Size: 110 1019 2020 187 2869 Fragments arranged by size: 2869 2020 1019 187 110 BsrGI T'GTAC_A Cuts at: 96 2733 96 Size: 2637 3568 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 4265 5277 4265 Size: 1012 5193 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6205 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstXI CCAn_nnnn'nTGG Cuts at: 3394 3394 Size: 6205 BstYI r'GATC_y Cuts at: 1972 2101 2673 3307 3535 3902 4287 4304 5756 Size: 129 572 634 228 367 385 17 1452 Cuts at: 5756 5842 5854 6200 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 634 572 385 367 346 228 129 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4714 Fragments arranged by size: 4714 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2509 3338 487 Size: 683 56 1283 829 3354 Fragments arranged by size: 3354 1283 829 683 56 BtsI GCAGTG_nn' Cuts at: 3721 4515 4535 3721 Size: 794 20 5391 Fragments arranged by size: 5391 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2694 2802 3652 Size: 54 1425 50 275 18 864 108 850 Cuts at: 3652 4541 6170 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 864 850 275 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3443 4135 1353 Size: 2090 692 3423 Fragments arranged by size: 3423 2090 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3373 4792 5166 5312 387 Size: 1064 138 1784 1419 374 146 1280 Fragments arranged by size: 1784 1419 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2785 2785 Size: 6205 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1996 2074 2204 2477 2603 2654 3182 Size: 393 364 78 130 273 126 51 528 Cuts at: 3182 3296 4268 4865 5652 5920 1239 Size: 114 972 597 787 268 1524 Fragments arranged by size: 1524 972 787 597 528 393 364 273 268 130 126 114 78 51 EcoNI CCTnn'n_nnAGG Cuts at: 2540 2540 Size: 6205 EcoO109I rG'GnC_Cy Cuts at: 2364 2378 2545 2629 3301 2364 Size: 14 167 84 672 5268 Fragments arranged by size: 5268 672 167 84 14 EcoRI G'AATT_C Cuts at: 3254 3514 3892 3254 Size: 260 378 5567 Fragments arranged by size: 5567 378 260 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 5352 1678 Size: 3674 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 3218 3250 4846 Size: 32 135 32 476 32 1138 32 1596 Cuts at: 4846 4878 4920 4952 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1596 1138 476 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3549 3795 669 Size: 988 281 1611 246 3079 Fragments arranged by size: 3079 1611 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KpnI G_GTAC'C Cuts at: 3083 3530 3083 Size: 447 5758 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2329 5319 5503 1305 Size: 1024 2990 184 2007 Fragments arranged by size: 2990 2007 1024 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2696 2804 10 Size: 54 1425 343 864 108 3411 Fragments arranged by size: 3411 1425 864 343 108 54 MslI CAynn'nnrTG Cuts at: 518 1166 2711 2798 3209 3392 4163 4522 4681 Size: 648 1545 87 411 183 771 359 159 Cuts at: 4681 518 Size: 2042 Fragments arranged by size: 2042 1545 771 648 411 359 183 159 87 MspA1I CmG'CkG Cuts at: 1606 3043 3621 4298 5446 5691 1606 Size: 1437 578 677 1148 245 2120 Fragments arranged by size: 2120 1437 1148 677 578 245 NaeI GCC'GGC Cuts at: 2831 2831 Size: 6205 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4714 Fragments arranged by size: 4714 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NgoMIV G'CCGG_C Cuts at: 2829 2829 Size: 6205 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 3563 5108 1132 Size: 97 2334 1545 2229 Fragments arranged by size: 2334 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3498 1348 Size: 2150 4055 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6205 PfoI T'CCnGG_A Cuts at: 2181 2181 Size: 6205 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3108 3140 4274 4306 5787 5819 3108 Size: 32 1134 32 1481 32 3494 Fragments arranged by size: 3494 1481 1134 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2364 2378 2545 3301 2364 Size: 14 167 756 5268 Fragments arranged by size: 5268 756 167 14 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 2963 3775 2963 Size: 812 5393 PstI C_TGCA'G Cuts at: 830 3048 3379 3557 830 Size: 2218 331 178 3478 Fragments arranged by size: 3478 2218 331 178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 PvuII CAG'CTG Cuts at: 3043 3043 Size: 6205 SacI G_AGCT'C Cuts at: 2787 2787 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 3044 3375 3553 4688 5369 5560 826 Size: 219 1999 331 178 1135 681 191 1471 Fragments arranged by size: 1999 1471 1135 681 331 219 191 178 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3532 1872 Size: 1660 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4309 5210 5472 820 Size: 197 926 2366 901 262 1553 Fragments arranged by size: 2366 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 2004 2604 3030 3644 513 Size: 552 939 600 426 614 3074 Fragments arranged by size: 3074 939 614 600 552 426 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 2327 2788 4232 4249 4402 4587 Size: 1255 726 21 461 1444 17 153 185 Cuts at: 4587 5032 325 Size: 445 1498 Fragments arranged by size: 1498 1444 1255 726 461 445 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2733 4451 6163 Size: 274 80 33 51 1443 756 1718 1712 Cuts at: 6163 96 Size: 138 Fragments arranged by size: 1718 1712 1443 756 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6165 6197 6165 Size: 32 6173 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2357 2578 2357 Size: 221 5984 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3530 1870 Size: 1660 4545 XmnI GAAnn'nnTTC Cuts at: 2639 2940 4334 2639 Size: 301 1394 4510 Fragments arranged by size: 4510 1394 301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI