(Linear) (Six-base) MAPSORT of: KIEE1413.seq Check: 5888 from: 1 to: 1500 KIEE1413.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. Acc65I G'GTAC_C Cuts at: 0 1074 1500 Size: 1074 426 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 649 1291 1500 Size: 472 177 642 209 Fragments arranged by size: 642 472 209 177 AleI CACnn'nnGTG Cuts at: 0 706 793 1500 Size: 706 87 707 Fragments arranged by size: 707 706 87 AlwNI CAG_nnn'CTG Cuts at: 0 376 543 627 1086 1354 1500 Size: 376 167 84 459 268 146 Fragments arranged by size: 459 376 268 167 146 84 ApoI r'AATT_y Cuts at: 0 1249 1500 Size: 1249 251 AvaI C'yCGr_G Cuts at: 0 547 1500 Size: 547 953 BamHI G'GATC_C Cuts at: 0 96 1500 Size: 96 1404 BanI G'GyrC_C Cuts at: 0 1074 1500 Size: 1074 426 BanII G_rGCy'C Cuts at: 0 281 782 1500 Size: 281 501 718 Fragments arranged by size: 718 501 281 BbsI GAAGACnn'nnnn_ Cuts at: 0 904 1109 1461 1500 Size: 904 205 352 39 Fragments arranged by size: 904 352 205 39 BglII A'GATC_T Cuts at: 0 668 1302 1500 Size: 668 634 198 Fragments arranged by size: 668 634 198 Bme1580I G_kGCm'C Cuts at: 0 968 1500 Size: 968 532 BmrI ACTGGGnnnn_n' Cuts at: 0 365 1364 1500 Size: 365 999 136 Fragments arranged by size: 999 365 136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 199 598 1177 1500 Size: 69 130 399 579 323 Fragments arranged by size: 579 399 323 130 69 Bpu10I CC'TnA_GC Cuts at: 0 751 1500 Size: 751 749 BsaI GGTCTCn'nnnn_ Cuts at: 0 484 1500 Size: 484 1016 BsaHI Gr'CG_yC Cuts at: 0 767 1500 Size: 767 733 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 321 351 1500 Size: 321 30 1149 Fragments arranged by size: 1149 321 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 51 856 1500 Size: 51 805 644 Fragments arranged by size: 805 644 51 BseYI C'CCAG_C Cuts at: 0 46 320 344 553 1500 Size: 46 274 24 209 947 Fragments arranged by size: 947 274 209 46 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1438 1500 Size: 1438 62 BsiHKAI G_wGCw'C Cuts at: 0 782 1500 Size: 782 718 BsmI GAATG_Cn' Cuts at: 0 717 1500 Size: 717 783 Bsp1286I G_dGCh'C Cuts at: 0 281 782 968 1500 Size: 281 501 186 532 Fragments arranged by size: 532 501 281 186 BsrDI GCAATG_nn' Cuts at: 0 482 1500 Size: 482 1018 BsrFI r'CCGG_y Cuts at: 0 824 1500 Size: 824 676 BsrGI T'GTAC_A Cuts at: 0 728 1500 Size: 728 772 BstXI CCAn_nnnn'nTGG Cuts at: 0 1389 1500 Size: 1389 111 BstYI r'GATC_y Cuts at: 0 96 668 1302 1500 Size: 96 572 634 198 Fragments arranged by size: 634 572 198 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 504 1333 1500 Size: 504 829 167 Fragments arranged by size: 829 504 167 EaeI y'GGCC_r Cuts at: 0 689 797 1500 Size: 689 108 703 Fragments arranged by size: 703 689 108 EarI CTCTTCn'nnn_ Cuts at: 0 1438 1500 Size: 1438 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1368 1500 Size: 1368 132 EcoICRI GAG'CTC Cuts at: 0 780 1500 Size: 780 720 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 199 472 598 649 1177 1291 1500 Size: 69 130 273 126 51 528 114 209 Fragments arranged by size: 528 273 209 130 126 114 69 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 535 1500 Size: 535 965 EcoO109I rG'GnC_Cy Cuts at: 0 359 373 540 624 1296 1500 Size: 359 14 167 84 672 204 Fragments arranged by size: 672 359 204 167 84 14 EcoRI G'AATT_C Cuts at: 0 1249 1500 Size: 1249 251 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 43 75 1213 1245 1500 Size: 43 32 1138 32 255 Fragments arranged by size: 1138 255 43 32 32 KpnI G_GTAC'C Cuts at: 0 1078 1500 Size: 1078 422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 324 1500 Size: 324 1176 MscI TGG'CCA Cuts at: 0 691 799 1500 Size: 691 108 701 Fragments arranged by size: 701 691 108 MslI CAynn'nnrTG Cuts at: 0 706 793 1204 1387 1500 Size: 706 87 411 183 113 Fragments arranged by size: 706 411 183 113 87 MspA1I CmG'CkG Cuts at: 0 1038 1500 Size: 1038 462 NaeI GCC'GGC Cuts at: 0 826 1500 Size: 826 674 NgoMIV G'CCGG_C Cuts at: 0 824 1500 Size: 824 676 PasI CC'CwG_GG Cuts at: 0 1493 1500 Size: 1493 7 PfoI T'CCnGG_A Cuts at: 0 176 1500 Size: 176 1324 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1103 1135 1500 Size: 1103 32 365 Fragments arranged by size: 1103 365 32 PpuMI rG'GwC_Cy Cuts at: 0 359 373 540 1296 1500 Size: 359 14 167 756 204 Fragments arranged by size: 756 359 204 167 14 PsiI TTA'TAA Cuts at: 0 958 1500 Size: 958 542 PstI C_TGCA'G Cuts at: 0 1043 1374 1500 Size: 1043 331 126 Fragments arranged by size: 1043 331 126 PvuII CAG'CTG Cuts at: 0 1038 1500 Size: 1038 462 SacI G_AGCT'C Cuts at: 0 782 1500 Size: 782 718 SfcI C'TryA_G Cuts at: 0 1039 1370 1500 Size: 1039 331 130 Fragments arranged by size: 1039 331 130 StyI C'CwwG_G Cuts at: 0 599 1025 1500 Size: 599 426 475 Fragments arranged by size: 599 475 426 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 301 322 783 1500 Size: 301 21 461 717 Fragments arranged by size: 717 461 301 21 TatI w'GTAC_w Cuts at: 0 728 1500 Size: 728 772 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 352 573 1500 Size: 352 221 927 Fragments arranged by size: 927 352 221 XmnI GAAnn'nnTTC Cuts at: 0 634 935 1500 Size: 634 301 565 Fragments arranged by size: 634 565 301 Enzymes that do cut: Acc65I AcuI AleI AlwNI ApoI AvaI BamHI BanI BanII BbsI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BsrGI BstXI BstYI BtgZI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV PasI PfoI PpiI PpuMI PsiI PstI PvuII SacI SfcI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI ZraI