################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspI AvrII BbeI BbrPI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII EcoO65I EcoRI EcoT22I FauI FbaI FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 829 GTCTAC GT^[AC][GT]AC AfaI 574 GTAC GT^AC AflIII 506 ACATGT A^C[GA][CT]GT AhdI 965 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 808 CCTGAGG CC^T[ACGT]AGG Aor51HI 1275 AGCGCT AGC^GCT AspEI 965 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1102 GTGCTC G[AT]GC[AT]^C AvaI 707 CTCGGG C^[CT]CG[GA]G AviII 353 TGCGCA TGC^GCA BamHI 200 GGATCC G^GATCC BbvCI 276 GCTGAGG CCTCAGC(-5/-2) BcgI 479 GCAGTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 703 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiEI 213 CGGCCG CG[GA][CT]^CG BsiHKAI 1102 GTGCTC G[AT]GC[AT]^C BsoBI 707 CTCGGG C^[CT]CG[GA]G BspLU11I 506 ACATGT A^CATGT BspMI 341 GCAGGT ACCTGC(4/8) BsrBI 1039 CCGCTC CCGCTC(-3/-3) BsrDI 41 CATTGC GCAATG(2/0) BstAPI 584 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 29 CCATACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 808 CCTGAGG CC^T[ACGT]AGG DrdI 948 GACCACCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 213 CGGCCG C^GGCCG Eam1105I 965 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 538 CTCTTC CTCTTC(1/4) EclXI 213 CGGCCG C^GGCCG Eco47III 1275 AGCGCT AGC^GCT Eco52I 213 CGGCCG C^GGCCG Eco81I 808 CCTGAGG CC^T[ACGT]AGG EcoRV 890 GATATC GAT^ATC FspI 353 TGCGCA TGC^GCA HincII 418 GTCAAC GT[CT]^[GA]AC HindII 418 GTCAAC GT[CT]^[GA]AC Ksp632I 538 CTCTTC CTCTTC(1/4) NspI 506 ACATGT [GA]CATG^[CT] PflMI 720 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 574 GTAC GT^AC ScaI 573 AGTACT AGT^ACT SmlI 195 CTTGAG C^T[CT][GA]AG StuI 280 AGGCCT AGG^CCT Tsp45I 482 GTGAC ^GT[GC]AC Van91I 720 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Asp700I 534 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 748 GAAATCTTTC AvaII 333 GGTCC G^G[AT]CC 1074 GGACC BanI 73 GGTGCC G^G[CT][GA]CC 1417 GGTGCC BanII 17 GAGCCC G[GA]GC[CT]^C 378 GGGCTC BbsI 853 GTCTTC GAAGAC(2/6) 1132 GTCTTC BpuAI 853 GTCTTC GAAGAC(2/6) 1132 GTCTTC BseRI 321 CTCCTC GAGGAG(10/8) 1279 CTCCTC BsmAI 515 GAGAC GTCTC(1/5) 305 GTCTC BspHI 797 TCATGA T^CATGA 879 TCATGA BstYI 200 GGATCC [GA]^GATC[CT] 1391 AGATCC Cac8I 36 GCTGGC GC[ACGT]^[ACGT]GC 865 GCCAGC DraII 4 GGGGCCT [GA]G^G[ACGT]CC[CT] 296 AGGGCCT DsaI 216 CCGTGG C^C[GA][CT]GG 976 CCACGG EcoNI 443 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 804 CCTTCCTGAGG EcoO109I 4 GGGGCCT [GA]G^G[ACGT]CC[CT] 296 AGGGCCT EcoT14I 8 CCTTGG C^C[AT][AT]GG 1380 CCTTGG HaeII 221 GGCGCT [GA]GCGC^[CT] 1275 AGCGCT MflI 200 GGATCC [GA]^GATC[CT] 1391 AGATCC MseI 740 TTAA T^TAA 1298 TTAA MslI 250 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1152 CACTAATGTG MspA1I 252 CAGCTG C[AC]G^C[GT]G 471 CAGCTG PvuII 252 CAGCTG CAG^CTG 471 CAGCTG RcaI 797 TCATGA T^CATGA 879 TCATGA StyI 8 CCTTGG C^C[AT][AT]GG 1380 CCTTGG TfiI 1062 GAATC G^A[AT]TC 1237 GAATC Tru9I 740 TTAA T^TAA 1298 TTAA XhoII 200 GGATCC [GA]^GATC[CT] 1391 AGATCC XmnI 534 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 748 GAAATCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AlwI AlwNI BalI BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI XcmI