(Circular) (Six-base) MAPSORT of: pFN21AE1939.seq Check: 9317 from: 1 to: 6148 pFN21AE1939 6148 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2337 2337 Size: 6148 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2835 3491 1937 Size: 898 656 4594 Fragments arranged by size: 4594 898 656 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3040 3230 3383 4211 5595 5863 1632 Size: 1408 190 153 828 1384 268 1917 Fragments arranged by size: 1917 1408 1384 828 268 190 153 AfeI AGC'GCT Cuts at: 3282 3282 Size: 6148 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 2511 5047 6024 1203 Size: 1308 2536 977 1327 Fragments arranged by size: 2536 1327 1308 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 2975 4877 2975 Size: 1902 4246 AleI CACnn'nnGTG Cuts at: 1166 2259 3161 1166 Size: 1093 902 4153 Fragments arranged by size: 4153 1093 902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3398 3430 3398 Size: 32 6116 AlwNI CAG_nnn'CTG Cuts at: 2305 2468 2731 3891 5463 2305 Size: 163 263 1160 1572 2990 Fragments arranged by size: 2990 1572 1160 263 163 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 3457 3683 3835 5944 1493 Size: 1964 226 152 2109 1697 Fragments arranged by size: 2109 1964 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 2712 3473 1870 Size: 73 769 761 4545 Fragments arranged by size: 4545 769 761 73 BamHI G'GATC_C Cuts at: 2205 3478 3845 2205 Size: 1273 367 4508 Fragments arranged by size: 4508 1273 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 3422 3469 4924 618 Size: 325 217 627 291 1344 47 1455 1842 Fragments arranged by size: 1842 1455 1344 627 325 291 217 47 BanII G_rGCy'C Cuts at: 1391 2026 2387 3467 1391 Size: 635 361 1080 4072 Fragments arranged by size: 4072 1080 635 361 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2851 3130 928 Size: 971 952 279 3946 Fragments arranged by size: 3946 971 952 279 BbvCI CC'TCA_GC Cuts at: 2282 2282 Size: 6148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2473 2507 4339 4373 1903 Size: 34 536 34 1832 34 3678 Fragments arranged by size: 3678 1832 536 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2714 4759 Size: 107 122 71 631 605 337 705 2045 Cuts at: 4759 136 Size: 1525 Fragments arranged by size: 2045 1525 705 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6143 6143 Size: 6148 BlpI GC'TnA_GC Cuts at: 3565 3565 Size: 6148 Bme1580I G_kGCm'C Cuts at: 1790 3036 4209 5365 1790 Size: 1246 1173 1156 2573 Fragments arranged by size: 2573 1246 1173 1156 BmgBI CAC'GTC Cuts at: 1318 2215 2500 1318 Size: 897 285 4966 Fragments arranged by size: 4966 897 285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3210 3879 4837 Size: 416 53 83 399 24 1793 669 958 Cuts at: 4837 442 Size: 1753 Fragments arranged by size: 1793 1753 958 669 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2458 2603 2753 2763 3005 3106 4808 1239 Size: 1219 145 150 10 242 101 1702 2579 Fragments arranged by size: 2579 1702 1219 242 150 145 101 10 Bpu10I CC'TnA_GC Cuts at: 2168 2282 2168 Size: 114 6034 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2221 4273 5138 5436 2221 Size: 2052 865 298 2933 Fragments arranged by size: 2933 2052 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4811 882 Size: 617 3312 2219 Fragments arranged by size: 3312 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3939 493 Size: 711 2735 2702 Fragments arranged by size: 2735 2702 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3955 4337 4972 275 Size: 53 83 186 936 2422 382 635 1451 Fragments arranged by size: 2422 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 4979 5253 5400 5811 4581 Size: 398 274 147 411 4918 Fragments arranged by size: 4918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2726 2756 1178 Size: 30 165 30 1323 30 4570 Fragments arranged by size: 4570 1323 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2317 3275 1186 Size: 1131 958 4059 Fragments arranged by size: 4059 1131 958 BseYI C'CCAG_C Cuts at: 3116 5351 5847 3116 Size: 2235 496 3417 Fragments arranged by size: 3417 2235 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5575 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2221 3598 4359 5387 1540 Size: 275 186 220 1377 761 1028 2301 Fragments arranged by size: 2301 1377 1028 761 275 220 186 BsiHKAI G_wGCw'C Cuts at: 3111 4209 4294 5365 3111 Size: 1098 85 1071 3894 Fragments arranged by size: 3894 1098 1071 85 BsmI GAATG_Cn' Cuts at: 3659 3752 3659 Size: 93 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2387 3036 3111 3467 4209 4294 Size: 399 236 361 649 75 356 742 85 Cuts at: 4294 5365 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 649 399 361 356 236 85 75 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 2802 2884 4037 5767 2802 Size: 82 1153 1730 3183 Fragments arranged by size: 3183 1730 1153 82 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2337 3503 844 Size: 1493 1166 3489 Fragments arranged by size: 3489 1493 1166 BsrBI CCG'CTC Cuts at: 1394 1823 3046 4035 1394 Size: 429 1223 989 3507 Fragments arranged by size: 3507 1223 989 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2045 4643 4817 65 Size: 1183 797 2598 174 1396 Fragments arranged by size: 2598 1396 1183 797 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 2595 794 Size: 1801 4347 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6148 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2041 Size: 6148 BstYI r'GATC_y Cuts at: 1972 2205 3396 3478 3845 4230 4247 5699 5785 Size: 233 1191 82 367 385 17 1452 86 Cuts at: 5785 5797 6143 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 233 86 82 17 12 Bsu36I CC'TnA_GG Cuts at: 2814 2814 Size: 6148 BtgI C'CryG_G Cuts at: 513 1065 2004 2221 2981 513 Size: 552 939 217 760 3680 Fragments arranged by size: 3680 939 760 552 217 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2476 487 Size: 683 56 1250 4159 Fragments arranged by size: 4159 1250 683 56 BtsI GCAGTG_nn' Cuts at: 2491 3664 4458 4478 2491 Size: 1173 794 20 4161 Fragments arranged by size: 4161 1173 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2959 5155 809 Size: 507 1643 2196 1802 Fragments arranged by size: 2196 1802 1643 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2218 2296 Size: 54 1425 50 275 18 186 202 78 Cuts at: 2296 2868 3153 3336 3595 4484 6113 8 Size: 572 285 183 259 889 1629 43 Fragments arranged by size: 1629 1425 889 572 285 275 259 202 186 183 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2218 3595 1537 Size: 275 406 1377 4090 Fragments arranged by size: 4090 1377 406 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2549 4078 1353 Size: 1196 1529 3423 Fragments arranged by size: 3423 1529 1196 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3157 4735 5109 5255 387 Size: 1064 138 1568 1578 374 146 1280 Fragments arranged by size: 1578 1568 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2458 2603 2753 2763 3005 3040 3106 Size: 393 826 145 150 10 242 35 66 Cuts at: 3106 3230 3383 4211 4808 5595 5863 1239 Size: 124 153 828 597 787 268 1524 Fragments arranged by size: 1524 828 826 787 597 393 268 242 153 150 145 124 66 35 10 EcoNI CCTnn'n_nnAGG Cuts at: 2452 2813 2452 Size: 361 5787 EcoO109I rG'GnC_Cy Cuts at: 2010 2302 2010 Size: 292 5856 EcoRI G'AATT_C Cuts at: 3457 3835 3457 Size: 378 5770 EcoRV GAT'ATC Cuts at: 2897 2897 Size: 6148 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2386 2386 Size: 6148 FspI TGC'GCA Cuts at: 2360 4654 2360 Size: 2294 3854 HaeII r_GCGC'y Cuts at: 1678 2230 3284 5295 1678 Size: 552 1054 2011 2531 Fragments arranged by size: 2531 2011 1054 552 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2961 2993 4789 4821 4863 Size: 32 135 32 1389 32 1796 32 42 Cuts at: 4863 4895 1373 Size: 32 2626 Fragments arranged by size: 2626 1796 1389 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 3492 3738 669 Size: 988 281 487 1067 246 3079 Fragments arranged by size: 3079 1067 988 487 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2346 2971 2992 3232 3255 5262 5446 1305 Size: 1041 625 21 240 23 2007 184 2007 Fragments arranged by size: 2007 2007 1041 625 240 184 23 21 MscI TGG'CCA Cuts at: 10 64 1489 1832 2018 2298 3155 10 Size: 54 1425 343 186 280 857 3003 Fragments arranged by size: 3003 1425 857 343 280 186 54 MslI CAynn'nnrTG Cuts at: 518 1166 2259 3161 4106 4465 4624 518 Size: 648 1093 902 945 359 159 2042 Fragments arranged by size: 2042 1093 945 902 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2259 2478 3564 4241 5389 5634 1606 Size: 653 219 1086 677 1148 245 2120 Fragments arranged by size: 2120 1148 1086 677 653 245 219 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4657 Fragments arranged by size: 4657 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 2515 3506 5051 1132 Size: 97 1286 991 1545 2229 Fragments arranged by size: 2229 1545 1286 991 97 PasI CC'CwG_GG Cuts at: 1348 3062 1348 Size: 1714 4434 PciI A'CATG_T Cuts at: 2511 5047 2511 Size: 2536 3612 PflMI CCAn_nnn'nTGG Cuts at: 1108 2731 1108 Size: 1623 4525 PfoI T'CCnGG_A Cuts at: 2450 2450 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2552 2584 4217 4249 5730 5762 2552 Size: 32 1633 32 1481 32 2938 Fragments arranged by size: 2938 1633 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2758 2790 2758 Size: 32 6116 PstI C_TGCA'G Cuts at: 830 2124 2157 2483 2800 3396 3500 830 Size: 1294 33 326 317 596 104 3478 Fragments arranged by size: 3478 1294 596 326 317 104 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2259 2478 2259 Size: 219 5929 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 2580 4396 6108 2580 Size: 1816 1712 2620 Fragments arranged by size: 2620 1816 1712 SfcI C'TryA_G Cuts at: 826 1045 2120 2153 2378 2479 2630 2796 3392 Size: 219 1075 33 225 101 151 166 596 Cuts at: 3392 3496 4631 5312 5503 826 Size: 104 1135 681 191 1471 Fragments arranged by size: 1471 1135 1075 681 596 225 219 191 166 151 104 101 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2200 4252 5153 5415 820 Size: 197 926 257 2052 901 262 1553 Fragments arranged by size: 2052 1553 926 901 262 257 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 2287 1517 Size: 770 5378 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 3385 3587 513 Size: 552 939 9 1372 202 3074 Fragments arranged by size: 3074 1372 939 552 202 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2066 2410 3410 4175 4192 4345 4530 Size: 1255 486 344 1000 765 17 153 185 Cuts at: 4530 4975 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1000 765 486 445 344 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2578 4394 6106 Size: 274 80 33 51 1443 601 1816 1712 Cuts at: 6106 96 Size: 138 Fragments arranged by size: 1816 1712 1443 601 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6108 6140 6108 Size: 32 6116 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2089 2219 3010 2011 Size: 78 130 791 5149 Fragments arranged by size: 5149 791 130 78 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 2543 2757 4277 2543 Size: 214 1520 4414 Fragments arranged by size: 4414 1520 214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI