(Linear) (Six-base) MAPSORT of: KIEE1939.seq Check: 4528 from: 1 to: 1443 KIEE1939.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 332 1443 Size: 332 1111 AccI GT'mk_AC Cuts at: 0 830 1443 Size: 830 613 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1035 1225 1378 1443 Size: 1035 190 153 65 Fragments arranged by size: 1035 190 153 65 AfeI AGC'GCT Cuts at: 0 1277 1443 Size: 1277 166 AflIII A'CryG_T Cuts at: 0 506 1443 Size: 506 937 AhdI GACnn_n'nnGTC Cuts at: 0 970 1443 Size: 970 473 AleI CACnn'nnGTG Cuts at: 0 254 1156 1443 Size: 254 902 287 Fragments arranged by size: 902 287 254 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1393 1425 1443 Size: 1393 32 18 Fragments arranged by size: 1393 32 18 AlwNI CAG_nnn'CTG Cuts at: 0 300 463 726 1443 Size: 300 163 263 717 Fragments arranged by size: 717 300 263 163 AvaI C'yCGr_G Cuts at: 0 707 1443 Size: 707 736 BamHI G'GATC_C Cuts at: 0 200 1443 Size: 200 1243 BanI G'GyrC_C Cuts at: 0 73 1417 1443 Size: 73 1344 26 Fragments arranged by size: 1344 73 26 BanII G_rGCy'C Cuts at: 0 21 382 1443 Size: 21 361 1061 Fragments arranged by size: 1061 361 21 BbsI GAAGACnn'nnnn_ Cuts at: 0 846 1125 1443 Size: 846 279 318 Fragments arranged by size: 846 318 279 BbvCI CC'TCA_GC Cuts at: 0 277 1443 Size: 277 1166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 468 502 1443 Size: 468 34 941 Fragments arranged by size: 941 468 34 BglI GCCn_nnn'nGGC Cuts at: 0 709 1443 Size: 709 734 Bme1580I G_kGCm'C Cuts at: 0 1031 1443 Size: 1031 412 BmgBI CAC'GTC Cuts at: 0 210 495 1443 Size: 210 285 948 Fragments arranged by size: 948 285 210 BmrI ACTGGGnnnn_n' Cuts at: 0 1205 1443 Size: 1205 238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 453 598 748 758 1000 1101 1443 Size: 453 145 150 10 242 101 342 Fragments arranged by size: 453 342 242 150 145 101 10 Bpu10I CC'TnA_GC Cuts at: 0 163 277 1443 Size: 163 114 1166 Fragments arranged by size: 1166 163 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 1443 Size: 216 1227 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 721 751 1443 Size: 721 30 692 Fragments arranged by size: 721 692 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 312 1270 1443 Size: 312 958 173 Fragments arranged by size: 958 312 173 BseYI C'CCAG_C Cuts at: 0 1111 1443 Size: 1111 332 BsiEI CG_ry'CG Cuts at: 0 216 1443 Size: 216 1227 BsiHKAI G_wGCw'C Cuts at: 0 1106 1443 Size: 1106 337 Bsp1286I G_dGCh'C Cuts at: 0 21 382 1031 1106 1443 Size: 21 361 649 75 337 Fragments arranged by size: 649 361 337 75 21 BspHI T'CATG_A Cuts at: 0 797 879 1443 Size: 797 82 564 Fragments arranged by size: 797 564 82 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 332 1443 Size: 332 1111 BsrBI CCG'CTC Cuts at: 0 1041 1443 Size: 1041 402 BsrDI GCAATG_nn' Cuts at: 0 40 1443 Size: 40 1403 BstAPI GCAn_nnn'nTGC Cuts at: 0 590 1443 Size: 590 853 BstXI CCAn_nnnn'nTGG Cuts at: 0 36 1443 Size: 36 1407 BstYI r'GATC_y Cuts at: 0 200 1391 1443 Size: 200 1191 52 Fragments arranged by size: 1191 200 52 Bsu36I CC'TnA_GG Cuts at: 0 809 1443 Size: 809 634 BtgI C'CryG_G Cuts at: 0 216 976 1443 Size: 216 760 467 Fragments arranged by size: 760 467 216 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 471 1443 Size: 471 972 BtsI GCAGTG_nn' Cuts at: 0 486 1443 Size: 486 957 DrdI GACnn_nn'nnGTC Cuts at: 0 954 1443 Size: 954 489 EaeI y'GGCC_r Cuts at: 0 11 213 291 863 1148 1331 1443 Size: 11 202 78 572 285 183 112 Fragments arranged by size: 572 285 202 183 112 78 11 EagI C'GGCC_G Cuts at: 0 213 1443 Size: 213 1230 EarI CTCTTCn'nnn_ Cuts at: 0 544 1443 Size: 544 899 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1152 1443 Size: 1152 291 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 453 598 748 758 1000 1035 1101 1225 Size: 453 145 150 10 242 35 66 124 Cuts at: 1225 1378 1443 Size: 153 65 Fragments arranged by size: 453 242 153 150 145 124 66 65 35 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 447 808 1443 Size: 447 361 635 Fragments arranged by size: 635 447 361 EcoO109I rG'GnC_Cy Cuts at: 0 5 297 1443 Size: 5 292 1146 Fragments arranged by size: 1146 292 5 EcoRV GAT'ATC Cuts at: 0 892 1443 Size: 892 551 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 381 1443 Size: 381 1062 FspI TGC'GCA Cuts at: 0 355 1443 Size: 355 1088 HaeII r_GCGC'y Cuts at: 0 225 1279 1443 Size: 225 1054 164 Fragments arranged by size: 1054 225 164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 956 988 1443 Size: 956 32 455 Fragments arranged by size: 956 455 32 HincII GTy'rAC Cuts at: 0 420 1443 Size: 420 1023 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 341 966 987 1227 1250 1443 Size: 341 625 21 240 23 193 Fragments arranged by size: 625 341 240 193 23 21 MscI TGG'CCA Cuts at: 0 13 293 1150 1443 Size: 13 280 857 293 Fragments arranged by size: 857 293 280 13 MslI CAynn'nnrTG Cuts at: 0 254 1156 1443 Size: 254 902 287 Fragments arranged by size: 902 287 254 MspA1I CmG'CkG Cuts at: 0 254 473 1443 Size: 254 219 970 Fragments arranged by size: 970 254 219 NspI r_CATG'y Cuts at: 0 510 1443 Size: 510 933 PasI CC'CwG_GG Cuts at: 0 1057 1443 Size: 1057 386 PciI A'CATG_T Cuts at: 0 506 1443 Size: 506 937 PflMI CCAn_nnn'nTGG Cuts at: 0 726 1443 Size: 726 717 PfoI T'CCnGG_A Cuts at: 0 445 1443 Size: 445 998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 547 579 1443 Size: 547 32 864 Fragments arranged by size: 864 547 32 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 753 785 1443 Size: 753 32 658 Fragments arranged by size: 753 658 32 PstI C_TGCA'G Cuts at: 0 119 152 478 795 1391 1443 Size: 119 33 326 317 596 52 Fragments arranged by size: 596 326 317 119 52 33 PvuII CAG'CTG Cuts at: 0 254 473 1443 Size: 254 219 970 Fragments arranged by size: 970 254 219 ScaI AGT'ACT Cuts at: 0 575 1443 Size: 575 868 SfcI C'TryA_G Cuts at: 0 115 148 373 474 625 791 1387 1443 Size: 115 33 225 101 151 166 596 56 Fragments arranged by size: 596 225 166 151 115 101 56 33 SmlI C'TyrA_G Cuts at: 0 195 1443 Size: 195 1248 StuI AGG'CCT Cuts at: 0 282 1443 Size: 282 1161 StyI C'CwwG_G Cuts at: 0 8 1380 1443 Size: 8 1372 63 Fragments arranged by size: 1372 63 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 61 405 1405 1443 Size: 61 344 1000 38 Fragments arranged by size: 1000 344 61 38 TatI w'GTAC_w Cuts at: 0 573 1443 Size: 573 870 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 84 214 1005 1443 Size: 84 130 791 438 Fragments arranged by size: 791 438 130 84 XmnI GAAnn'nnTTC Cuts at: 0 538 752 1443 Size: 538 214 691 Fragments arranged by size: 691 538 214 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AhdI AleI AloI AlwNI AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NspI PasI PciI PflMI PfoI PpiI PsrI PstI PvuII ScaI SfcI SmlI StuI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglII BlpI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII EcoICRI EcoRI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI ZraI