################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI BalI BamHI BanII BbeI BbrPI BbvCI BcgI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FseI Hin1I HpaI KasI KpnI KspI MaeII MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 82 GTCGAC GT^[AC][GT]AC AflII 1413 CTTAAG C^TTAAG AhdI 206 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 1275 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 206 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1771 GAGCAC G[AT]GC[AT]^C AvaI 4 CCCGAG C^[CT]CG[GA]G AviII 149 TGCGCA TGC^GCA AvrII 1212 CCTAGG C^CTAGG BanI 1530 GGCACC G^G[CT][GA]CC BbsI 547 GAAGAC GAAGAC(2/6) BfrI 1413 CTTAAG C^TTAAG BglI 31 GCCTCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 738 AGATCT A^GATCT BlnI 1212 CCTAGG C^CTAGG BpuAI 547 GAAGAC GAAGAC(2/6) BsaI 322 GAGACC GGTCTC(1/5) BsaJI 1212 CCTAGG C^C[ACGT][ACGT]GG BsgI 64 CTGCAC GTGCAG(16/14) BsiHKAI 1771 GAGCAC G[AT]GC[AT]^C BsoBI 4 CCCGAG C^[CT]CG[GA]G Bsp1407I 1571 TGTACA T^GTACA BsrGI 1571 TGTACA T^GTACA BstXI 1091 CCAAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 173 AGGACCT [GA]G^G[ACGT]CC[CT] DrdI 772 GACCTATTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 206 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 173 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1053 GAATTC G^AATTC EcoT14I 1212 CCTAGG C^C[AT][AT]GG EcoT22I 1786 ATGCAT ATGCA^T FspI 149 TGCGCA TGC^GCA HaeII 1662 GGCGCT [GA]GCGC^[CT] HincII 82 GTCGAC GT[CT]^[GA]AC HindII 82 GTCGAC GT[CT]^[GA]AC HindIII 1226 AAGCTT A^AGCTT MfeI 1495 CAATTG C^AATTG MslI 1018 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1495 CAATTG C^AATTG NlaIV 1530 GGCACC GG[ACGT]^[ACGT]CC NsiI 1786 ATGCAT ATGCA^T NspI 1784 GCATGC [GA]CATG^[CT] PleI 1389 GACTC GAGTC(4/5) PpuMI 173 AGGACCT [GA]G^G[AT]CC[CT] SalI 82 GTCGAC G^TCGAC SfaNI 1788 GCATC GCATC(5/9) SmlI 1413 CTTAAG C^T[CT][GA]AG SphI 1784 GCATGC GCATG^C SspBI 1571 TGTACA T^GTACA StyI 1212 CCTAGG C^C[AT][AT]GG SwaI 1448 ATTTAAAT ATTT^AAAT TaqI 83 TCGA T^CGA TthHB8I 83 TCGA T^CGA XmnI 1275 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 989 GCGG CCGC(-3/-1) 1620 GCGG AfaI 969 GTAC GT^AC 1572 GTAC BciVI 15 GGATAC GTATCC(6/5) 165 GGATAC BclI 197 TGATCA T^GATCA 1746 TGATCA BcnI 107 CCGGG CC^[GC]GG 1703 CCCGG BfaI 916 CTAG C^TAG 1213 CTAG BpmI 346 CTCCAG CTGGAG(16/14) 1522 CTCCAG BsrDI 879 CATTGC GCAATG(2/0) 1801 GCAATG DraI 1297 TTTAAA TTT^AAA 1449 TTTAAA FbaI 197 TGATCA T^GATCA 1746 TGATCA HphI 1003 GGTGA GGTGA(8/7) 1088 TCACC MaeI 916 CTAG C^TAG 1213 CTAG NciI 107 CCGGG CC^[GC]GG 1703 CCCGG RsaI 969 GTAC GT^AC 1572 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AcsI AluI AlwI ApoI AvaII BbvI BmyI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII PstI Sau3AI Sau96I ScrFI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII