(Circular) (Six-base) MAPSORT of: pFN21AE2028.seq Check: 1226 from: 1 to: 6538 pFN21AE2028 6538 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2076 2082 2674 2076 Size: 6 592 5940 Fragments arranged by size: 5940 592 6 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4348 278 Size: 53 83 186 936 2812 2468 Fragments arranged by size: 2812 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2088 3881 1937 Size: 151 1793 4594 Fragments arranged by size: 4594 1793 151 Acc65I G'GTAC_C Cuts at: 3859 3859 Size: 6538 AclI AA'CG_TT Cuts at: 1563 4665 5038 1563 Size: 3102 373 3063 Fragments arranged by size: 3102 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3129 3500 4601 5985 6253 1632 Size: 1497 371 1101 1384 268 1917 Fragments arranged by size: 1917 1497 1384 1101 371 268 AflII C'TTAA_G Cuts at: 820 1017 3418 820 Size: 197 2401 3940 Fragments arranged by size: 3940 2401 197 AflIII A'CryG_T Cuts at: 1203 5437 6414 1203 Size: 4234 977 1327 Fragments arranged by size: 4234 1327 977 AgeI A'CCGG_T Cuts at: 5369 5369 Size: 6538 AhdI GACnn_n'nnGTC Cuts at: 2216 5267 2216 Size: 3051 3487 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6538 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6538 AlwNI CAG_nnn'CTG Cuts at: 4281 5853 4281 Size: 1572 4966 ApaLI G'TGCA_C Cuts at: 4595 5751 4595 Size: 1156 5382 ApoI r'AATT_y Cuts at: 1493 2316 2576 3058 3457 3847 4073 4225 6334 Size: 823 260 482 399 390 226 152 2109 Cuts at: 6334 1493 Size: 1697 Fragments arranged by size: 2109 1697 823 482 399 390 260 226 152 AseI AT'TA_AT Cuts at: 160 1032 5092 160 Size: 872 4060 1606 Fragments arranged by size: 4060 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 AvaI C'yCGr_G Cuts at: 1870 1943 2009 3863 1870 Size: 73 66 1854 4545 Fragments arranged by size: 4545 1854 73 66 AvrII C'CTAG_G Cuts at: 3217 3217 Size: 6538 BamHI G'GATC_C Cuts at: 3868 4235 3868 Size: 367 6171 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3535 3859 5314 618 Size: 325 217 627 1748 324 1455 1842 Fragments arranged by size: 1842 1748 1455 627 325 324 217 BanII G_rGCy'C Cuts at: 1391 3857 1391 Size: 2466 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2559 928 Size: 971 660 4907 Fragments arranged by size: 4907 971 660 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4729 4763 1903 Size: 34 50 34 2708 34 3678 Fragments arranged by size: 3678 2708 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2014 2164 4430 5640 2014 Size: 150 2266 1210 2912 Fragments arranged by size: 2912 2266 1210 150 BclI T'GATC_A Cuts at: 1548 2202 3751 1548 Size: 654 1549 4335 Fragments arranged by size: 4335 1549 654 BfrBI ATG'CAT Cuts at: 3793 3793 Size: 6538 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 5149 136 Size: 107 122 71 631 605 370 3107 1525 Fragments arranged by size: 3107 1525 631 605 370 122 107 71 BglII A'GATC_T Cuts at: 2743 6533 2743 Size: 3790 2748 BlpI GC'TnA_GC Cuts at: 3955 3955 Size: 6538 Bme1580I G_kGCm'C Cuts at: 1790 3538 3555 4599 5755 1790 Size: 1748 17 1044 1156 2573 Fragments arranged by size: 2573 1748 1156 1044 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6538 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4269 5227 442 Size: 416 53 83 399 24 2852 958 1753 Fragments arranged by size: 2852 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6538 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3555 3555 Size: 6538 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 3512 5198 1239 Size: 1097 1176 1686 2579 Fragments arranged by size: 2579 1686 1176 1097 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4663 5528 5826 4663 Size: 865 298 5375 Fragments arranged by size: 5375 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2321 5201 882 Size: 617 822 2880 2219 Fragments arranged by size: 2880 2219 822 617 BsaAI yAC'GTr Cuts at: 493 1204 4329 493 Size: 711 3125 2702 Fragments arranged by size: 3125 2702 711 BsaBI GATnn'nnATC Cuts at: 6532 6532 Size: 6538 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4345 4727 5362 275 Size: 53 83 186 936 2812 382 635 1451 Fragments arranged by size: 2812 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4971 5369 5643 5790 6201 4971 Size: 398 274 147 411 5308 Fragments arranged by size: 5308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3510 3540 1178 Size: 30 165 30 2107 30 4176 Fragments arranged by size: 4176 2107 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6538 BseYI C'CCAG_C Cuts at: 5741 6237 5741 Size: 496 6042 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 1163 Size: 573 318 5647 Fragments arranged by size: 5647 573 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3988 4749 5777 1540 Size: 275 186 1987 761 1028 2301 Fragments arranged by size: 2301 1987 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3780 4599 4684 5755 3780 Size: 819 85 1071 4563 Fragments arranged by size: 4563 1071 819 85 BsmI GAATG_Cn' Cuts at: 3346 3570 3633 4049 4142 3346 Size: 224 63 416 93 5742 Fragments arranged by size: 5742 416 224 93 63 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3538 3555 3780 3857 4599 4684 5755 Size: 399 1748 17 225 77 742 85 1071 Cuts at: 5755 1391 Size: 2174 Fragments arranged by size: 2174 1748 1071 742 399 225 85 77 17 BspEI T'CCGG_A Cuts at: 6201 6201 Size: 6538 BspHI T'CATG_A Cuts at: 4427 6157 4427 Size: 1730 4808 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 2082 2674 3893 844 Size: 1232 6 592 1219 3489 Fragments arranged by size: 3489 1232 1219 592 6 BsrBI CCG'CTC Cuts at: 1394 1823 4425 1394 Size: 429 2602 3507 Fragments arranged by size: 3507 2602 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2883 3813 5033 5207 65 Size: 1183 1635 930 1220 174 1396 Fragments arranged by size: 1635 1396 1220 1183 930 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5182 5369 1700 Size: 110 3372 187 2869 Fragments arranged by size: 3372 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3576 96 Size: 3480 3058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6538 BssSI C'ACGA_G Cuts at: 4598 5610 4598 Size: 1012 5526 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6538 BstBI TT'CG_AA Cuts at: 5342 5342 Size: 6538 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6538 BstXI CCAn_nnnn'nTGG Cuts at: 3103 3103 Size: 6538 BstYI r'GATC_y Cuts at: 1972 2743 3484 3724 3868 4235 4620 4637 6089 Size: 771 741 240 144 367 385 17 1452 Cuts at: 6089 6175 6187 6533 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 771 741 385 367 346 240 144 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5986 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5799 Fragments arranged by size: 5799 683 56 BtsI GCAGTG_nn' Cuts at: 2422 4054 4848 4868 2422 Size: 1632 794 20 4092 Fragments arranged by size: 4092 1632 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6538 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3626 3661 3795 3830 529 Size: 35 3062 35 134 35 3237 Fragments arranged by size: 3237 3062 134 35 35 35 DraI TTT'AAA Cuts at: 3304 3456 3842 3304 Size: 152 386 6000 Fragments arranged by size: 6000 386 152 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2783 5545 809 Size: 507 1467 2762 1802 Fragments arranged by size: 2762 1802 1467 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3985 4874 6503 Size: 54 1425 50 275 18 2155 889 1629 Cuts at: 6503 8 Size: 43 Fragments arranged by size: 2155 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3985 1537 Size: 275 2173 4090 Fragments arranged by size: 4090 2173 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2280 3496 3546 4468 1353 Size: 927 1216 50 922 3423 Fragments arranged by size: 3423 1216 927 922 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5125 5499 5645 387 Size: 1064 138 3536 374 146 1280 Fragments arranged by size: 3536 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 3129 3500 3512 4601 5198 5985 Size: 393 704 793 371 12 1089 597 787 Cuts at: 5985 6253 1239 Size: 268 1524 Fragments arranged by size: 1524 1089 793 787 704 597 393 371 268 12 EcoO109I rG'GnC_Cy Cuts at: 2179 2179 Size: 6538 EcoRI G'AATT_C Cuts at: 3058 3847 4225 3058 Size: 789 378 5371 Fragments arranged by size: 5371 789 378 FspI TGC'GCA Cuts at: 2156 5044 2156 Size: 2888 3650 HaeII r_GCGC'y Cuts at: 1678 3671 5685 1678 Size: 1993 2014 2531 Fragments arranged by size: 2531 2014 1993 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2107 2139 2202 2234 2281 Size: 32 135 32 535 32 63 32 47 Cuts at: 2281 2313 2705 2737 2902 2934 5179 5211 5253 Size: 32 392 32 165 32 2245 32 42 Cuts at: 5253 5285 1373 Size: 32 2626 Fragments arranged by size: 2626 2245 535 392 165 135 63 47 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2089 3882 4128 669 Size: 988 281 151 1793 246 3079 Fragments arranged by size: 3079 1793 988 281 246 151 HindIII A'AGCT_T Cuts at: 748 3231 748 Size: 2483 4055 HpaI GTT'AAC Cuts at: 4128 4128 Size: 6538 KpnI G_GTAC'C Cuts at: 3863 3863 Size: 6538 MfeI C'AATT_G Cuts at: 3500 4137 3500 Size: 637 5901 MluI A'CGCG_T Cuts at: 6414 6414 Size: 6538 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2605 5652 5836 1305 Size: 1300 3047 184 2007 Fragments arranged by size: 3047 2007 1300 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4716 Fragments arranged by size: 4716 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3027 4496 4855 5014 518 Size: 648 1861 1469 359 159 2042 Fragments arranged by size: 2042 1861 1469 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3954 4631 5779 6024 1606 Size: 2348 677 1148 245 2120 Fragments arranged by size: 2348 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5986 NdeI CA'TA_TG Cuts at: 387 387 Size: 6538 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6538 NotI GC'GGCC_GC Cuts at: 3985 3985 Size: 6538 NsiI A_TGCA'T Cuts at: 3795 3795 Size: 6538 NspI r_CATG'y Cuts at: 1132 1229 3793 3896 5441 1132 Size: 97 2564 103 1545 2229 Fragments arranged by size: 2564 2229 1545 103 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6538 PciI A'CATG_T Cuts at: 5437 5437 Size: 6538 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6538 PfoI T'CCnGG_A Cuts at: 3358 3358 Size: 6538 PmeI GTTT'AAAC Cuts at: 3842 3842 Size: 6538 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4607 4639 6120 6152 4607 Size: 32 1481 32 4993 Fragments arranged by size: 4993 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2179 2179 Size: 6538 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6538 PsiI TTA'TAA Cuts at: 4108 4108 Size: 6538 PstI C_TGCA'G Cuts at: 830 2084 2429 2482 3890 830 Size: 1254 345 53 1408 3478 Fragments arranged by size: 3478 1408 1254 345 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6538 SalI G'TCGA_C Cuts at: 1936 2087 3880 1936 Size: 151 1793 4594 Fragments arranged by size: 4594 1793 151 SbfI CC_TGCA'GG Cuts at: 3890 3890 Size: 6538 ScaI AGT'ACT Cuts at: 4786 6498 4786 Size: 1712 4826 SfcI C'TryA_G Cuts at: 826 1045 2080 2425 2478 3053 3886 5021 5702 Size: 219 1035 345 53 575 833 1135 681 Cuts at: 5702 5893 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1035 833 681 575 345 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6538 SmaI CCC'GGG Cuts at: 1872 3865 1872 Size: 1993 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3418 4642 5543 5805 820 Size: 197 926 1475 1224 901 262 1553 Fragments arranged by size: 1553 1475 1224 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4329 493 Size: 3836 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6538 SphI G_CATG'C Cuts at: 1132 3793 3896 1132 Size: 2661 103 3774 Fragments arranged by size: 3774 2661 103 SspI AAT'ATT Cuts at: 5 52 4462 5 Size: 47 4410 2081 Fragments arranged by size: 4410 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6538 StyI C'CwwG_G Cuts at: 513 1065 3217 3977 513 Size: 552 2152 760 3074 Fragments arranged by size: 3074 2152 760 552 SwaI ATTT'AAAT Cuts at: 3456 3456 Size: 6538 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2406 4565 4582 4735 4920 5365 325 Size: 1255 826 2159 17 153 185 445 1498 Fragments arranged by size: 2159 1498 1255 826 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2973 3576 4784 Size: 274 80 33 51 1443 996 603 1208 Cuts at: 4784 6496 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1208 996 603 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2784 2816 2955 2987 3510 3542 6498 6530 2784 Size: 32 139 32 523 32 2956 32 2792 Fragments arranged by size: 2956 2792 523 139 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6538 XbaI T'CTAG_A Cuts at: 3874 3874 Size: 6538 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6538 XmaI C'CCGG_G Cuts at: 1870 3863 1870 Size: 1993 4545 XmnI GAAnn'nnTTC Cuts at: 3284 4667 3284 Size: 1383 5155 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4346 276 Size: 53 83 186 936 2812 2468 Fragments arranged by size: 2812 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI