(Linear) (Six-base) MAPSORT of: KIEE2028.seq Check: 6931 from: 1 to: 1833 KIEE2028.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 71 77 669 1833 Size: 71 6 592 1164 Fragments arranged by size: 1164 592 71 6 AccI GT'mk_AC Cuts at: 0 83 1833 Size: 83 1750 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1124 1495 1833 Size: 1124 371 338 Fragments arranged by size: 1124 371 338 AflII C'TTAA_G Cuts at: 0 1413 1833 Size: 1413 420 AhdI GACnn_n'nnGTC Cuts at: 0 211 1833 Size: 211 1622 ApoI r'AATT_y Cuts at: 0 311 571 1053 1452 1833 Size: 311 260 482 399 381 Fragments arranged by size: 482 399 381 311 260 AvaI C'yCGr_G Cuts at: 0 4 1833 Size: 4 1829 AvrII C'CTAG_G Cuts at: 0 1212 1833 Size: 1212 621 BanI G'GyrC_C Cuts at: 0 1530 1833 Size: 1530 303 BbsI GAAGACnn'nnnn_ Cuts at: 0 554 1833 Size: 554 1279 BciVI GTATCCnnnnn_n' Cuts at: 0 9 159 1833 Size: 9 150 1674 Fragments arranged by size: 1674 150 9 BclI T'GATC_A Cuts at: 0 197 1746 1833 Size: 197 1549 87 Fragments arranged by size: 1549 197 87 BfrBI ATG'CAT Cuts at: 0 1788 1833 Size: 1788 45 BglI GCCn_nnn'nGGC Cuts at: 0 37 1833 Size: 37 1796 BglII A'GATC_T Cuts at: 0 738 1833 Size: 738 1095 Bme1580I G_kGCm'C Cuts at: 0 1533 1550 1833 Size: 1533 17 283 Fragments arranged by size: 1533 283 17 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1550 1833 Size: 1550 283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 1507 1833 Size: 331 1176 326 Fragments arranged by size: 1176 331 326 BsaI GGTCTCn'nnnn_ Cuts at: 0 316 1833 Size: 316 1517 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1505 1535 1833 Size: 1505 30 298 Fragments arranged by size: 1505 298 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 1833 Size: 49 1784 BsiHKAI G_wGCw'C Cuts at: 0 1775 1833 Size: 1775 58 BsmI GAATG_Cn' Cuts at: 0 1341 1565 1628 1833 Size: 1341 224 63 205 Fragments arranged by size: 1341 224 205 63 Bsp1286I G_dGCh'C Cuts at: 0 1533 1550 1775 1833 Size: 1533 17 225 58 Fragments arranged by size: 1533 225 58 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 71 77 669 1833 Size: 71 6 592 1164 Fragments arranged by size: 1164 592 71 6 BsrDI GCAATG_nn' Cuts at: 0 878 1808 1833 Size: 878 930 25 Fragments arranged by size: 930 878 25 BsrGI T'GTAC_A Cuts at: 0 1571 1833 Size: 1571 262 BstXI CCAn_nnnn'nTGG Cuts at: 0 1098 1833 Size: 1098 735 BstYI r'GATC_y Cuts at: 0 738 1479 1719 1833 Size: 738 741 240 114 Fragments arranged by size: 741 738 240 114 BtsI GCAGTG_nn' Cuts at: 0 417 1833 Size: 417 1416 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1621 1656 1790 1825 1833 Size: 1621 35 134 35 8 Fragments arranged by size: 1621 134 35 35 8 DraI TTT'AAA Cuts at: 0 1299 1451 1833 Size: 1299 152 382 Fragments arranged by size: 1299 382 152 DrdI GACnn_nn'nnGTC Cuts at: 0 778 1833 Size: 778 1055 EarI CTCTTCn'nnn_ Cuts at: 0 275 1491 1541 1833 Size: 275 1216 50 292 Fragments arranged by size: 1216 292 275 50 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 1124 1495 1507 1833 Size: 331 793 371 12 326 Fragments arranged by size: 793 371 331 326 12 EcoO109I rG'GnC_Cy Cuts at: 0 174 1833 Size: 174 1659 EcoRI G'AATT_C Cuts at: 0 1053 1833 Size: 1053 780 FspI TGC'GCA Cuts at: 0 151 1833 Size: 151 1682 HaeII r_GCGC'y Cuts at: 0 1666 1833 Size: 1666 167 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 102 134 197 229 276 308 700 732 Size: 102 32 63 32 47 32 392 32 Cuts at: 732 897 929 1833 Size: 165 32 904 Fragments arranged by size: 904 392 165 102 63 47 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 84 1833 Size: 84 1749 HindIII A'AGCT_T Cuts at: 0 1226 1833 Size: 1226 607 MfeI C'AATT_G Cuts at: 0 1495 1833 Size: 1495 338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 600 1833 Size: 600 1233 MslI CAynn'nnrTG Cuts at: 0 1022 1833 Size: 1022 811 NsiI A_TGCA'T Cuts at: 0 1790 1833 Size: 1790 43 NspI r_CATG'y Cuts at: 0 1788 1833 Size: 1788 45 PfoI T'CCnGG_A Cuts at: 0 1353 1833 Size: 1353 480 PpuMI rG'GwC_Cy Cuts at: 0 174 1833 Size: 174 1659 PstI C_TGCA'G Cuts at: 0 79 424 477 1833 Size: 79 345 53 1356 Fragments arranged by size: 1356 345 79 53 SalI G'TCGA_C Cuts at: 0 82 1833 Size: 82 1751 SfcI C'TryA_G Cuts at: 0 75 420 473 1048 1833 Size: 75 345 53 575 785 Fragments arranged by size: 785 575 345 75 53 SmlI C'TyrA_G Cuts at: 0 1413 1833 Size: 1413 420 SphI G_CATG'C Cuts at: 0 1788 1833 Size: 1788 45 StyI C'CwwG_G Cuts at: 0 1212 1833 Size: 1212 621 SwaI ATTT'AAAT Cuts at: 0 1451 1833 Size: 1451 382 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 401 1833 Size: 401 1432 TatI w'GTAC_w Cuts at: 0 968 1571 1833 Size: 968 603 262 Fragments arranged by size: 968 603 262 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 779 811 950 982 1505 1537 1833 Size: 779 32 139 32 523 32 296 Fragments arranged by size: 779 523 296 139 32 32 32 XmnI GAAnn'nnTTC Cuts at: 0 1279 1833 Size: 1279 554 Enzymes that do cut: AarI AccI AcuI AflII AhdI ApoI AvaI AvrII BanI BbsI BciVI BclI BfrBI BglI BglII Bme1580I BplI BpmI BsaI BsaXI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstXI BstYI BtsI CspCI DraI DrdI EarI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII MfeI MmeI MslI NsiI NspI PfoI PpuMI PstI SalI SfcI SmlI SphI StyI SwaI TaqII TatI TstI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BanII BbeI BbvCI BcgI BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI DraIII EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspAI HpaI KasI KpnI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI Tth111I XbaI XcmI XhoI XmaI ZraI