################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MaeII MluI MluNI MroI MscI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 481 GTCTAC GT^[AC][GT]AC AcsI 1699 GAATTT [GA]^AATT[CT] ApoI 1699 GAATTT [GA]^AATT[CT] AvaII 1000 GGACC G^G[AT]CC BanI 233 GGTGCC G^G[CT][GA]CC BanII 289 GAGCCC G[GA]GC[CT]^C BbvCI 1384 GCTGAGG CCTCAGC(-5/-2) BsaBI 877 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1194 ACCGGA [AT]^CCGG[AT] BsmFI 817 GGGAC GGGAC(10/14) BspMI 1002 ACCTGC ACCTGC(4/8) BsrBI 10 GAGCGG CCGCTC(-3/-3) BssSI 98 CTCGTG CACGAG(-5/-1) CfoI 24 GCGC GCG^C DraI 739 TTTAAA TTT^AAA DraII 263 AGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 1836 GACATCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1684 CCGTGG C^C[GA][CT]GG EarI 286 GAAGAG CTCTTC(1/4) EcoO109I 263 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 962 GATATC GAT^ATC EcoT22I 1213 ATGCAT ATGCA^T FauI 407 GCGGG CCCGC(4/6) HhaI 24 GCGC GCG^C HinP1I 24 GCGC G^CGC HincII 1321 GTTGAC GT[CT]^[GA]AC HindII 1321 GTTGAC GT[CT]^[GA]AC Ksp632I 286 GAAGAG CTCTTC(1/4) MamI 877 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1301 CAATTG C^AATTG MspA1I 1084 CAGCTG C[AC]G^C[GT]G MunI 1301 CAATTG C^AATTG NsiI 1213 ATGCAT ATGCA^T PvuII 1084 CAGCTG CAG^CTG SapI 286 GAAGAGC GCTCTTC(1/4) StuI 1388 AGGCCT AGG^CCT XbaI 426 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccII 25 CGCG CG^CG 406 CGCG AlwI 346 GATCC GGATC(4/5) 781 GATCC AlwNI 354 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG 1408 CAGGCACTG BpmI 351 CTCCAG CTGGAG(16/14) 1766 CTCCAG BsmAI 717 GAGAC GTCTC(1/5) 1834 GAGAC Bsp1407I 807 TGTACA T^GTACA 1574 TGTACA BsrDI 1188 CATTGC GCAATG(2/0) 1561 CATTGC BsrGI 807 TGTACA T^GTACA 1574 TGTACA BssKI 111 CCAGG ^CC[ACGT]GG 441 CCTGG BstNI 111 CCAGG CC^[AT]GG 441 CCTGG BstUI 25 CGCG CG^CG 406 CGCG BstYI 345 AGATCC [GA]^GATC[CT] 780 AGATCC Cfr13I 264 GGCCC G^G[ACGT]CC 1000 GGACC EcoRII 111 CCAGG ^CC[AT]GG 441 CCTGG HaeIII 264 GGCC GG^CC 1389 GGCC HapII 30 CCGG C^CGG 1195 CCGG HgaI 819 GACGC GACGC(5/10) 26 GCGTC HpaII 30 CCGG C^CGG 1195 CCGG HphI 1582 GGTGA GGTGA(8/7) 1678 TCACC MflI 345 AGATCC [GA]^GATC[CT] 780 AGATCC MspI 30 CCGG C^CGG 1195 CCGG MvaI 111 CCAGG CC^[AT]GG 441 CCTGG MvnI 25 CGCG CG^CG 406 CGCG NlaIV 233 GGTGCC GG[ACGT]^[ACGT]CC 1332 GGAACC PflMI 353 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1053 CCAAGCGATGG PstI 1362 CTGCAG CTGCA^G 1721 CTGCAG Sau96I 264 GGCCC G^G[ACGT]CC 1000 GGACC ScrFI 111 CCAGG CC^[ACGT]GG 441 CCTGG SmlI 985 CTTGAG C^T[CT][GA]AG 1755 CTCAAG SspBI 807 TGTACA T^GTACA 1574 TGTACA TspRI 911 CAGTG CA[GC]TG[ACGT][ACGT]^ 1412 CACTG Van91I 353 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1053 CCAAGCGATGG XcmI 1506 CCACACAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1775 CCAGACCTTCCTTGG XhoII 345 AGATCC [GA]^GATC[CT] 780 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AluI AspHI BbsI BbvI BfaI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BstF5I Cac8I DdeI DpnII EcoT14I Fnu4HI FokI HindIII HinfI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NspI RsaI Sau3AI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TthHB8I