################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrFI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI Eam1105I EarI Eco47III EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KpnI Ksp632I KspI MamI MluI MluNI MscI NaeI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccIII 149 TCCGGA T^CCGGA AcyI 205 GGCGCC G[GA]^CG[CT]C AocI 1507 CCTAAGG CC^T[ACGT]AGG Asp700I 1215 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 205 GGCGCC GGCGC^C BclI 399 TGATCA T^GATCA BcnI 76 CCCGG CC^[GC]GG BfaI 854 CTAG C^TAG BglII 220 AGATCT A^GATCT BsaHI 205 GGCGCC G[GA]^CG[CT]C BsaWI 149 TCCGGA [AT]^CCGG[AT] BseAI 149 TCCGGA T^CCGGA BseRI 19 CTCCTC GAGGAG(10/8) BsgI 893 CTGCAC GTGCAG(16/14) BsmAI 1353 GTCTC GTCTC(1/5) BsmFI 35 GTCCC GGGAC(10/14) BspEI 149 TCCGGA T^CCGGA BsrBI 302 GAGCGG CCGCTC(-3/-3) BssHII 25 GCGCGC G^CGCGC BstAPI 1405 GCAAAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 220 AGATCT [GA]^GATC[CT] Bsu36I 1507 CCTAAGG CC^T[ACGT]AGG DraI 846 TTTAAA TTT^AAA DsaI 1142 CCATGG C^C[GA][CT]GG Eco81I 1507 CCTAAGG CC^T[ACGT]AGG EcoT14I 1142 CCATGG C^C[AT][AT]GG FauI 45 GCGGG CCCGC(4/6) FbaI 399 TGATCA T^GATCA HaeII 205 GGCGCC [GA]GCGC^[CT] Hin1I 205 GGCGCC G[GA]^CG[CT]C HindIII 281 AAGCTT A^AGCTT KasI 205 GGCGCC G^GCGCC MaeI 854 CTAG C^TAG MaeII 696 ACGT A^CGT MboII 1008 GAAGA GAAGA(8/7) MfeI 482 CAATTG C^AATTG MflI 220 AGATCT [GA]^GATC[CT] MroI 149 TCCGGA T^CCGGA MunI 482 CAATTG C^AATTG NarI 205 GGCGCC GG^CGCC NciI 76 CCCGG CC^[GC]GG NcoI 1142 CCATGG C^CATGG NotI 304 GCGGCCGC GC^GGCCGC PflMI 1155 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1351 GAGTC GAGTC(4/5) PstI 996 CTGCAG CTGCA^G PvuII 818 CAGCTG CAG^CTG ScaI 615 AGTACT AGT^ACT SfoI 205 GGCGCC GGC^GCC SphI 260 GCATGC GCATG^C StyI 1142 CCATGG C^C[AT][AT]GG Van91I 1155 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 220 AGATCT [GA]^GATC[CT] XmnI 1215 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AvaII 1042 GGTCC G^G[AT]CC 1522 GGTCC BanI 183 GGTGCC G^G[CT][GA]CC 205 GGCGCC BpmI 50 CTCCAG CTGGAG(16/14) 821 CTGGAG BsaJI 1045 CCTCGG C^C[ACGT][ACGT]GG 1142 CCATGG BsiEI 92 CGGCCG CG[GA][CT]^CG 305 CGGCCG BspHI 609 TCATGA T^CATGA 951 TCATGA BsrI 427 ACTGG ACTGG(1/-1) 271 CCAGT BssSI 40 CTCGTG CACGAG(-5/-1) 412 CTCGTG BstXI 321 CCAGGATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1259 CCAGGCTGCTGG Cfr13I 1042 GGTCC G^G[ACGT]CC 1522 GGTCC EaeI 92 CGGCCG [CT]^GGCC[GA] 305 CGGCCG EagI 92 CGGCCG C^GGCCG 305 CGGCCG EclXI 92 CGGCCG C^GGCCG 305 CGGCCG Eco52I 92 CGGCCG C^GGCCG 305 CGGCCG EcoNI 1045 CCTCGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1381 CCTCATATAGG HapII 77 CCGG C^CGG 150 CCGG HgaI 955 GACGC GACGC(5/10) 33 GCGTC HpaII 77 CCGG C^CGG 150 CCGG MslI 576 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1426 CACAAAAATG MspA1I 308 CCGCTG C[AC]G^C[GT]G 818 CAGCTG MspI 77 CCGG C^CGG 150 CCGG NspI 260 GCATGC [GA]CATG^[CT] 1514 ACATGC RcaI 609 TCATGA T^CATGA 951 TCATGA Sau96I 1042 GGTCC G^G[ACGT]CC 1522 GGTCC SfcI 631 CTACAG C^T[GA][CT]AG 996 CTGCAG TfiI 803 GAATC G^A[AT]TC 1310 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI BanII BbvI BmyI BsiHKAI BsiYI BslI Bsp1286I BsrDI BssKI BstF5I BstNI BstUI Cac8I CfoI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI SacI Sau3AI ScrFI SfaNI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I