(Circular) (Six-base) MAPSORT of: pFN21AE1954.seq Check: 9382 from: 1 to: 6235 pFN21AE1954 6235 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3578 1937 Size: 1641 4594 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2337 2409 3267 4298 5682 5950 1632 Size: 705 72 858 1031 1384 268 1917 Fragments arranged by size: 1917 1384 1031 858 705 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 5134 6111 1203 Size: 3931 977 1327 Fragments arranged by size: 3931 1327 977 AgeI A'CCGG_T Cuts at: 5066 5066 Size: 6235 AhdI GACnn_n'nnGTC Cuts at: 4964 4964 Size: 6235 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2613 3122 1184 Size: 1429 509 4297 Fragments arranged by size: 4297 1429 509 AlwNI CAG_nnn'CTG Cuts at: 3978 5550 3978 Size: 1572 4663 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 2146 2744 2936 3544 3770 3922 6031 1493 Size: 653 598 192 608 226 152 2109 1697 Fragments arranged by size: 2109 1697 653 608 598 226 192 152 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 3560 1870 Size: 73 1617 4545 Fragments arranged by size: 4545 1617 73 BamHI G'GATC_C Cuts at: 3565 3932 3565 Size: 367 5868 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2188 2210 3556 5011 618 Size: 325 217 627 401 22 1346 1455 1842 Fragments arranged by size: 1842 1455 1346 627 401 325 217 22 BanII G_rGCy'C Cuts at: 1391 2056 2564 2820 2976 3554 1391 Size: 665 508 256 156 578 4072 Fragments arranged by size: 4072 665 578 508 256 156 BbeI G_GCGC'C Cuts at: 2214 2214 Size: 6235 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4426 4460 1903 Size: 34 2489 34 3678 Fragments arranged by size: 3678 2489 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4127 5337 4127 Size: 1210 5025 BclI T'GATC_A Cuts at: 1548 2404 1548 Size: 856 5379 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4846 136 Size: 107 122 71 631 605 3174 1525 Fragments arranged by size: 3174 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2225 6230 2225 Size: 4005 2230 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 2106 4296 5452 1790 Size: 316 2190 1156 2573 Fragments arranged by size: 2573 2190 1156 316 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2270 3966 4924 Size: 416 53 83 399 24 853 1696 958 Cuts at: 4924 442 Size: 1753 Fragments arranged by size: 1753 1696 958 853 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2040 2847 4895 1239 Size: 801 807 2048 2579 Fragments arranged by size: 2579 2048 807 801 Bpu10I CC'TnA_GC Cuts at: 2068 2068 Size: 6235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4360 5225 5523 4360 Size: 865 298 5072 Fragments arranged by size: 5072 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4898 882 Size: 617 3399 2219 Fragments arranged by size: 3399 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4026 493 Size: 711 2822 2702 Fragments arranged by size: 2822 2702 711 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2211 4042 4424 5059 Size: 53 83 186 936 678 1831 382 635 Cuts at: 5059 275 Size: 1451 Fragments arranged by size: 1831 1451 936 678 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2154 4668 5066 5340 5487 5898 2154 Size: 2514 398 274 147 411 2491 Fragments arranged by size: 2514 2491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2007 2037 1178 Size: 30 165 30 604 30 5376 Fragments arranged by size: 5376 604 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 1186 Size: 829 5406 BseYI C'CCAG_C Cuts at: 3271 3523 5438 5934 3271 Size: 252 1915 496 3572 Fragments arranged by size: 3572 1915 496 252 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2883 1163 Size: 573 1147 4515 Fragments arranged by size: 4515 1147 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2100 2313 3685 4446 5474 1540 Size: 275 186 99 213 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 213 186 99 BsiHKAI G_wGCw'C Cuts at: 2017 2564 2820 2976 4296 4381 5452 2017 Size: 547 256 156 1320 85 1071 2800 Fragments arranged by size: 2800 1320 1071 547 256 156 85 BsmI GAATG_Cn' Cuts at: 3746 3839 3746 Size: 93 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2056 2106 2564 2820 2976 3554 Size: 399 227 39 50 458 256 156 578 Cuts at: 3554 4296 4381 5452 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 578 458 399 256 227 156 85 50 39 BspEI T'CCGG_A Cuts at: 2154 5898 2154 Size: 3744 2491 BspHI T'CATG_A Cuts at: 2614 2956 4124 5854 2614 Size: 342 1168 1730 2995 Fragments arranged by size: 2995 1730 1168 342 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3590 844 Size: 2746 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2309 4122 1394 Size: 429 486 1813 3507 Fragments arranged by size: 3507 1813 486 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2592 2784 3147 3208 4730 4904 65 Size: 1183 1344 192 363 61 1522 174 1396 Fragments arranged by size: 1522 1396 1344 1183 363 192 174 61 BsrFI r'CCGG_y Cuts at: 1700 1810 4879 5066 1700 Size: 110 3069 187 2869 Fragments arranged by size: 3069 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 2030 1925 Size: 105 6130 BssSI C'ACGA_G Cuts at: 2045 2417 4295 5307 2045 Size: 372 1878 1012 2973 Fragments arranged by size: 2973 1878 1012 372 BstAPI GCAn_nnn'nTGC Cuts at: 794 3416 794 Size: 2622 3613 BstBI TT'CG_AA Cuts at: 5039 5039 Size: 6235 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6235 BstXI CCAn_nnnn'nTGG Cuts at: 2333 3271 2333 Size: 938 5297 BstYI r'GATC_y Cuts at: 1972 2225 3565 3932 4317 4334 5786 5872 5884 Size: 253 1340 367 385 17 1452 86 12 Cuts at: 5884 6230 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1340 385 367 346 253 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3513 3513 Size: 6235 BtgI C'CryG_G Cuts at: 513 1065 3147 513 Size: 552 2082 3601 Fragments arranged by size: 3601 2082 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5496 Fragments arranged by size: 5496 683 56 BtsI GCAGTG_nn' Cuts at: 2832 2922 3751 4545 4565 2832 Size: 90 829 794 20 4502 Fragments arranged by size: 4502 829 794 90 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6200 DraI TTT'AAA Cuts at: 2853 3539 2853 Size: 686 5549 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5242 809 Size: 507 3926 1802 Fragments arranged by size: 3926 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2310 3682 Size: 54 1425 50 275 18 267 213 1372 Cuts at: 3682 4571 6200 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1372 889 275 267 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2097 2310 3682 1537 Size: 275 285 213 1372 4090 Fragments arranged by size: 4090 1372 285 275 213 EarI CTCTTCn'nnn_ Cuts at: 1353 4165 1353 Size: 2812 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4822 5196 5342 387 Size: 1064 138 3233 374 146 1280 Fragments arranged by size: 3233 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2562 2818 2974 2562 Size: 256 156 5823 Fragments arranged by size: 5823 256 156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2337 2409 2847 3267 4298 4895 Size: 393 408 297 72 438 420 1031 597 Cuts at: 4895 5682 5950 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1031 787 597 438 420 408 393 297 268 72 EcoNI CCTnn'n_nnAGG Cuts at: 3054 3390 3054 Size: 336 5899 EcoRI G'AATT_C Cuts at: 3544 3922 3544 Size: 378 5857 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 2214 5382 1678 Size: 536 3168 2531 Fragments arranged by size: 3168 2531 536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2651 2683 3057 3089 4876 Size: 32 135 32 1079 32 374 32 1787 Cuts at: 4876 4908 4950 4982 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1787 1079 374 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3579 3825 669 Size: 988 281 1641 246 3079 Fragments arranged by size: 3079 1641 988 281 246 HindIII A'AGCT_T Cuts at: 748 2286 748 Size: 1538 4697 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KasI G'GCGC_C Cuts at: 2210 2210 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 2487 3834 2487 Size: 1347 4888 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3172 5349 5533 1305 Size: 1867 2177 184 2007 Fragments arranged by size: 2177 2007 1867 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 3435 4193 4552 4711 518 Size: 648 1419 850 758 359 159 2042 Fragments arranged by size: 2042 1419 850 758 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2315 2825 3651 4328 5476 5721 1606 Size: 709 510 826 677 1148 245 2120 Fragments arranged by size: 2120 1148 826 709 677 510 245 NarI GG'CG_CC Cuts at: 2211 2211 Size: 6235 NcoI C'CATG_G Cuts at: 513 1065 3147 513 Size: 552 2082 3601 Fragments arranged by size: 3601 2082 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6235 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 2310 3682 2310 Size: 1372 4863 NspI r_CATG'y Cuts at: 1132 1229 2269 3523 3593 5138 1132 Size: 97 1040 1254 70 1545 2229 Fragments arranged by size: 2229 1545 1254 1040 97 70 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6235 PciI A'CATG_T Cuts at: 5134 5134 Size: 6235 PflMI CCAn_nnn'nTGG Cuts at: 1108 3166 1108 Size: 2058 4177 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PstI C_TGCA'G Cuts at: 830 3005 3587 830 Size: 2175 582 3478 Fragments arranged by size: 3478 2175 582 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 PvuII CAG'CTG Cuts at: 2825 2825 Size: 6235 SacI G_AGCT'C Cuts at: 2564 2820 2976 2564 Size: 256 156 5823 Fragments arranged by size: 5823 256 156 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 2622 4483 6195 2622 Size: 1861 1712 2662 Fragments arranged by size: 2662 1861 1712 SfcI C'TryA_G Cuts at: 826 1045 2636 3001 3583 4718 5399 5590 826 Size: 219 1591 365 582 1135 681 191 1471 Fragments arranged by size: 1591 1471 1135 681 582 365 219 191 SfoI GGC'GCC Cuts at: 2212 2212 Size: 6235 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 3562 1872 Size: 1690 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4339 5240 5502 820 Size: 197 926 2396 901 262 1553 Fragments arranged by size: 2396 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 2269 3593 1132 Size: 1137 1324 3774 Fragments arranged by size: 3774 1324 1137 SspI AAT'ATT Cuts at: 5 52 4159 5 Size: 47 4107 2081 Fragments arranged by size: 4107 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6235 StyI C'CwwG_G Cuts at: 513 1065 3147 3674 513 Size: 552 2082 527 3074 Fragments arranged by size: 3074 2082 552 527 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 3042 4262 4279 4432 4617 5062 Size: 1255 498 964 1220 17 153 185 445 Cuts at: 5062 325 Size: 1498 Fragments arranged by size: 1498 1255 1220 964 498 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2620 4481 6193 Size: 274 80 33 51 1443 643 1861 1712 Cuts at: 6193 96 Size: 138 Fragments arranged by size: 1861 1712 1443 643 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2007 2039 6195 6227 2007 Size: 32 4156 32 2015 Fragments arranged by size: 4156 2015 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 3560 1870 Size: 1690 4545 XmnI GAAnn'nnTTC Cuts at: 3224 4364 3224 Size: 1140 5095 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI