(Linear) (Six-base) MAPSORT of: KIEE1954.seq Check: 5641 from: 1 to: 1530 KIEE1954.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 404 1262 1530 Size: 332 72 858 268 Fragments arranged by size: 858 332 268 72 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 608 1117 1530 Size: 608 509 413 Fragments arranged by size: 608 509 413 ApoI r'AATT_y Cuts at: 0 141 739 931 1530 Size: 141 598 192 599 Fragments arranged by size: 599 598 192 141 BanI G'GyrC_C Cuts at: 0 183 205 1530 Size: 183 22 1325 Fragments arranged by size: 1325 183 22 BanII G_rGCy'C Cuts at: 0 51 559 815 971 1530 Size: 51 508 256 156 559 Fragments arranged by size: 559 508 256 156 51 BbeI G_GCGC'C Cuts at: 0 209 1530 Size: 209 1321 BclI T'GATC_A Cuts at: 0 399 1530 Size: 399 1131 BglII A'GATC_T Cuts at: 0 220 1530 Size: 220 1310 Bme1580I G_kGCm'C Cuts at: 0 101 1530 Size: 101 1429 BmrI ACTGGGnnnn_n' Cuts at: 0 265 1530 Size: 265 1265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 842 1530 Size: 35 807 688 Fragments arranged by size: 807 688 35 Bpu10I CC'TnA_GC Cuts at: 0 63 1530 Size: 63 1467 BsaHI Gr'CG_yC Cuts at: 0 206 1530 Size: 206 1324 BsaWI w'CCGG_w Cuts at: 0 149 1530 Size: 149 1381 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2 32 1530 Size: 2 30 1498 Fragments arranged by size: 1498 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 10 1530 Size: 10 1520 BseYI C'CCAG_C Cuts at: 0 1266 1518 1530 Size: 1266 252 12 Fragments arranged by size: 1266 252 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 878 1530 Size: 878 652 BsiEI CG_ry'CG Cuts at: 0 95 308 1530 Size: 95 213 1222 Fragments arranged by size: 1222 213 95 BsiHKAI G_wGCw'C Cuts at: 0 12 559 815 971 1530 Size: 12 547 256 156 559 Fragments arranged by size: 559 547 256 156 12 Bsp1286I G_dGCh'C Cuts at: 0 12 51 101 559 815 971 1530 Size: 12 39 50 458 256 156 559 Fragments arranged by size: 559 458 256 156 50 39 12 BspEI T'CCGG_A Cuts at: 0 149 1530 Size: 149 1381 BspHI T'CATG_A Cuts at: 0 609 951 1530 Size: 609 342 579 Fragments arranged by size: 609 579 342 BsrBI CCG'CTC Cuts at: 0 304 1530 Size: 304 1226 BsrDI GCAATG_nn' Cuts at: 0 587 779 1142 1203 1530 Size: 587 192 363 61 327 Fragments arranged by size: 587 363 327 192 61 BssHII G'CGCG_C Cuts at: 0 25 1530 Size: 25 1505 BssSI C'ACGA_G Cuts at: 0 40 412 1530 Size: 40 372 1118 Fragments arranged by size: 1118 372 40 BstAPI GCAn_nnn'nTGC Cuts at: 0 1411 1530 Size: 1411 119 BstXI CCAn_nnnn'nTGG Cuts at: 0 328 1266 1530 Size: 328 938 264 Fragments arranged by size: 938 328 264 BstYI r'GATC_y Cuts at: 0 220 1530 Size: 220 1310 Bsu36I CC'TnA_GG Cuts at: 0 1508 1530 Size: 1508 22 BtgI C'CryG_G Cuts at: 0 1142 1530 Size: 1142 388 BtsI GCAGTG_nn' Cuts at: 0 827 917 1530 Size: 827 90 613 Fragments arranged by size: 827 613 90 DraI TTT'AAA Cuts at: 0 848 1530 Size: 848 682 EaeI y'GGCC_r Cuts at: 0 92 305 1530 Size: 92 213 1225 Fragments arranged by size: 1225 213 92 EagI C'GGCC_G Cuts at: 0 92 305 1530 Size: 92 213 1225 Fragments arranged by size: 1225 213 92 EcoICRI GAG'CTC Cuts at: 0 557 813 969 1530 Size: 557 256 156 561 Fragments arranged by size: 561 557 256 156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 332 404 842 1262 1530 Size: 35 297 72 438 420 268 Fragments arranged by size: 438 420 297 268 72 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 1049 1385 1530 Size: 1049 336 145 Fragments arranged by size: 1049 336 145 HaeII r_GCGC'y Cuts at: 0 209 1530 Size: 209 1321 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 646 678 1052 1084 1530 Size: 646 32 374 32 446 Fragments arranged by size: 646 446 374 32 32 HindIII A'AGCT_T Cuts at: 0 281 1530 Size: 281 1249 KasI G'GCGC_C Cuts at: 0 205 1530 Size: 205 1325 MfeI C'AATT_G Cuts at: 0 482 1530 Size: 482 1048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1167 1530 Size: 1167 363 MslI CAynn'nnrTG Cuts at: 0 580 1430 1530 Size: 580 850 100 Fragments arranged by size: 850 580 100 MspA1I CmG'CkG Cuts at: 0 310 820 1530 Size: 310 510 710 Fragments arranged by size: 710 510 310 NarI GG'CG_CC Cuts at: 0 206 1530 Size: 206 1324 NcoI C'CATG_G Cuts at: 0 1142 1530 Size: 1142 388 NotI GC'GGCC_GC Cuts at: 0 305 1530 Size: 305 1225 NspI r_CATG'y Cuts at: 0 264 1518 1530 Size: 264 1254 12 Fragments arranged by size: 1254 264 12 PflMI CCAn_nnn'nTGG Cuts at: 0 1161 1530 Size: 1161 369 PstI C_TGCA'G Cuts at: 0 1000 1530 Size: 1000 530 PvuII CAG'CTG Cuts at: 0 820 1530 Size: 820 710 SacI G_AGCT'C Cuts at: 0 559 815 971 1530 Size: 559 256 156 559 Fragments arranged by size: 559 559 256 156 ScaI AGT'ACT Cuts at: 0 617 1530 Size: 617 913 SfcI C'TryA_G Cuts at: 0 631 996 1530 Size: 631 365 534 Fragments arranged by size: 631 534 365 SfoI GGC'GCC Cuts at: 0 207 1530 Size: 207 1323 SphI G_CATG'C Cuts at: 0 264 1530 Size: 264 1266 StyI C'CwwG_G Cuts at: 0 1142 1530 Size: 1142 388 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 73 1037 1530 Size: 73 964 493 Fragments arranged by size: 964 493 73 TatI w'GTAC_w Cuts at: 0 615 1530 Size: 615 915 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2 34 1530 Size: 2 32 1496 Fragments arranged by size: 1496 32 2 XmnI GAAnn'nnTTC Cuts at: 0 1219 1530 Size: 1219 311 Enzymes that do cut: AcuI AlfI ApoI BanI BanII BbeI BclI BglII Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BsrBI BsrDI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI EaeI EagI EcoICRI Eco57MI EcoNI HaeII Hin4I HindIII KasI MfeI MmeI MslI MspA1I NarI NcoI NotI NspI PflMI PstI PvuII SacI ScaI SfcI SfoI SphI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspMI BsrFI BsrGI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI EarI EciI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MluI MscI NaeI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI