(Circular) (Six-base) MAPSORT of: pFN21AE0334.seq Check: 6333 from: 1 to: 7330 pFN21AE0334 7330 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5140 278 Size: 53 83 186 936 3604 2468 Fragments arranged by size: 3604 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4673 1937 Size: 2736 4594 Acc65I G'GTAC_C Cuts at: 4651 4651 Size: 7330 AclI AA'CG_TT Cuts at: 1563 4629 5457 5830 1563 Size: 3066 828 373 3063 Fragments arranged by size: 3066 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2649 3579 3670 5393 6777 7045 1632 Size: 1017 930 91 1723 1384 268 1917 Fragments arranged by size: 1917 1723 1384 1017 930 268 91 AfeI AGC'GCT Cuts at: 2709 2709 Size: 7330 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7133 AflIII A'CryG_T Cuts at: 1203 2698 3231 4506 6229 7206 1203 Size: 1495 533 1275 1723 977 1327 Fragments arranged by size: 1723 1495 1327 1275 977 533 AgeI A'CCGG_T Cuts at: 6161 6161 Size: 7330 AhdI GACnn_n'nnGTC Cuts at: 6059 6059 Size: 7330 AleI CACnn'nnGTG Cuts at: 1166 3978 1166 Size: 2812 4518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4587 1184 Size: 3403 3927 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2738 2770 2738 Size: 32 7298 AlwNI CAG_nnn'CTG Cuts at: 3001 3478 4072 5073 6645 3001 Size: 477 594 1001 1572 3686 Fragments arranged by size: 3686 1572 1001 594 477 ApaLI G'TGCA_C Cuts at: 5387 6543 5387 Size: 1156 6174 ApoI r'AATT_y Cuts at: 1493 2318 2517 3903 4205 4358 4639 4865 5017 Size: 825 199 1386 302 153 281 226 152 Cuts at: 5017 7126 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1386 825 302 281 226 199 153 152 AseI AT'TA_AT Cuts at: 160 1032 4599 5884 160 Size: 872 3567 1285 1606 Fragments arranged by size: 3567 1606 1285 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 AvaI C'yCGr_G Cuts at: 1870 1943 4655 1870 Size: 73 2712 4545 Fragments arranged by size: 4545 2712 73 BamHI G'GATC_C Cuts at: 2746 3510 4660 5027 2746 Size: 764 1150 367 5049 Fragments arranged by size: 5049 1150 764 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3646 4651 6106 618 Size: 325 217 627 1859 1005 1455 1842 Fragments arranged by size: 1859 1842 1455 1005 627 325 217 BanII G_rGCy'C Cuts at: 1391 2721 4649 1391 Size: 1330 1928 4072 Fragments arranged by size: 4072 1928 1330 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2586 928 Size: 971 687 5672 Fragments arranged by size: 5672 971 687 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2246 2280 5521 5555 1903 Size: 34 309 34 3241 34 3678 Fragments arranged by size: 3678 3241 309 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2675 5222 6432 2675 Size: 2547 1210 3573 Fragments arranged by size: 3573 2547 1210 BclI T'GATC_A Cuts at: 1548 2428 4366 1548 Size: 880 1938 4512 Fragments arranged by size: 4512 1938 880 BfrBI ATG'CAT Cuts at: 2171 2738 2171 Size: 567 6763 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5941 136 Size: 107 122 71 631 605 4269 1525 Fragments arranged by size: 4269 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7325 7325 Size: 7330 BlpI GC'TnA_GC Cuts at: 2955 4747 2955 Size: 1792 5538 Bme1580I G_kGCm'C Cuts at: 1790 5391 6547 1790 Size: 3601 1156 2573 Fragments arranged by size: 3601 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7330 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2125 5061 6019 Size: 416 53 83 399 24 708 2936 958 Cuts at: 6019 442 Size: 1753 Fragments arranged by size: 2936 1753 958 708 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7330 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2999 3947 5990 1239 Size: 1760 948 2043 2579 Fragments arranged by size: 2579 2043 1760 948 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2437 5455 6320 6618 2437 Size: 3018 865 298 3149 Fragments arranged by size: 3149 3018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5993 882 Size: 617 4494 2219 Fragments arranged by size: 4494 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4507 5121 493 Size: 711 3303 614 2702 Fragments arranged by size: 3303 2702 711 614 BsaBI GATnn'nnATC Cuts at: 3969 4251 7324 3969 Size: 282 3073 3975 Fragments arranged by size: 3975 3073 282 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5137 5519 6154 275 Size: 53 83 186 936 3604 382 635 1451 Fragments arranged by size: 3604 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3768 5763 6161 6435 6582 6993 3768 Size: 1995 398 274 147 411 4105 Fragments arranged by size: 4105 1995 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3566 3596 1178 Size: 30 165 30 2163 30 4912 Fragments arranged by size: 4912 2163 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3443 4215 1186 Size: 2257 772 4301 Fragments arranged by size: 4301 2257 772 BseYI C'CCAG_C Cuts at: 2021 2055 4023 6533 7029 2021 Size: 34 1968 2510 496 2322 Fragments arranged by size: 2510 2322 1968 496 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2289 3116 3779 3936 1163 Size: 573 553 827 663 157 4557 Fragments arranged by size: 4557 827 663 573 553 157 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 4780 5541 6569 1540 Size: 275 186 93 2686 761 1028 2301 Fragments arranged by size: 2686 2301 1028 761 275 186 93 BsiHKAI G_wGCw'C Cuts at: 2453 5391 5476 6547 2453 Size: 2938 85 1071 3236 Fragments arranged by size: 3236 2938 1071 85 BsmI GAATG_Cn' Cuts at: 2799 4841 4934 2799 Size: 2042 93 5195 Fragments arranged by size: 5195 2042 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2453 2721 4649 5391 5476 6547 1391 Size: 399 663 268 1928 742 85 1071 2174 Fragments arranged by size: 2174 1928 1071 742 663 399 268 85 BspEI T'CCGG_A Cuts at: 3768 6993 3768 Size: 3225 4105 BspHI T'CATG_A Cuts at: 2431 5219 6949 2431 Size: 2788 1730 2812 Fragments arranged by size: 2812 2788 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4685 844 Size: 3841 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2011 3887 5217 1394 Size: 429 188 1876 1330 3507 Fragments arranged by size: 3507 1876 1330 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 3938 4281 5825 5999 65 Size: 1183 2690 343 1544 174 1396 Fragments arranged by size: 2690 1544 1396 1183 343 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 2325 5974 6161 1700 Size: 110 205 310 3649 187 2869 Fragments arranged by size: 3649 2869 310 205 187 110 BsrGI T'GTAC_A Cuts at: 96 3362 3725 4185 96 Size: 3266 363 460 3241 Fragments arranged by size: 3266 3241 460 363 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7330 BssSI C'ACGA_G Cuts at: 2265 5390 6402 2265 Size: 3125 1012 3193 Fragments arranged by size: 3193 3125 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2177 794 Size: 1383 5947 BstBI TT'CG_AA Cuts at: 6134 6134 Size: 7330 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7330 BstXI CCAn_nnnn'nTGG Cuts at: 2161 3562 2161 Size: 1401 5929 BstYI r'GATC_y Cuts at: 1972 2746 3510 4246 4660 5027 5412 5429 6881 Size: 774 764 736 414 367 385 17 1452 Cuts at: 6881 6967 6979 7325 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 774 764 736 414 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2533 3549 3976 513 Size: 552 939 529 1016 427 3867 Fragments arranged by size: 3867 1016 939 552 529 427 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6591 Fragments arranged by size: 6591 683 56 BtsI GCAGTG_nn' Cuts at: 2972 3347 3543 4846 5640 5660 2972 Size: 375 196 1303 794 20 4642 Fragments arranged by size: 4642 1303 794 375 196 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7330 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7295 DraI TTT'AAA Cuts at: 4634 4634 Size: 7330 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4507 6337 809 Size: 507 3191 1830 1802 Fragments arranged by size: 3191 1830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3552 4612 4777 Size: 54 1425 50 275 18 1722 1060 165 Cuts at: 4777 5666 7295 8 Size: 889 1629 43 Fragments arranged by size: 1722 1629 1425 1060 889 275 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4777 1537 Size: 275 2965 4090 Fragments arranged by size: 4090 2965 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2826 5260 1353 Size: 1473 2434 3423 Fragments arranged by size: 3423 2434 1473 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5917 6291 6437 387 Size: 1064 138 4328 374 146 1280 Fragments arranged by size: 4328 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2649 2999 3579 3670 3947 5393 5990 Size: 393 1017 350 580 91 277 1446 597 Cuts at: 5990 6777 7045 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1446 1017 787 597 580 393 350 277 268 91 EcoO109I rG'GnC_Cy Cuts at: 4495 4567 4495 Size: 72 7258 EcoRI G'AATT_C Cuts at: 4639 5017 4639 Size: 378 6952 EcoRV GAT'ATC Cuts at: 2544 2544 Size: 7330 FseI GG_CCGG'CC Cuts at: 2019 2019 Size: 7330 FspI TGC'GCA Cuts at: 5836 5836 Size: 7330 HaeII r_GCGC'y Cuts at: 1678 2711 6477 1678 Size: 1033 3766 2531 Fragments arranged by size: 3766 2531 1033 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 2148 2180 2435 Size: 32 135 32 500 32 44 32 255 Cuts at: 2435 2467 3566 3598 3611 3643 5971 6003 6045 Size: 32 1099 32 13 32 2328 32 42 Cuts at: 6045 6077 1373 Size: 32 2626 Fragments arranged by size: 2626 2328 1099 500 255 135 44 42 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 2770 4674 4920 669 Size: 988 281 832 1904 246 3079 Fragments arranged by size: 3079 1904 988 832 281 246 HindIII A'AGCT_T Cuts at: 748 3483 748 Size: 2735 4595 HpaI GTT'AAC Cuts at: 4920 4920 Size: 7330 KpnI G_GTAC'C Cuts at: 4655 4655 Size: 7330 MfeI C'AATT_G Cuts at: 4929 4929 Size: 7330 MluI A'CGCG_T Cuts at: 7206 7206 Size: 7330 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6444 6628 1305 Size: 5139 184 2007 Fragments arranged by size: 5139 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3554 10 Size: 54 1425 343 1722 3786 Fragments arranged by size: 3786 1722 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2493 2532 3092 3524 3978 5288 Size: 648 1035 292 39 560 432 454 1310 Cuts at: 5288 5647 5806 518 Size: 359 159 2042 Fragments arranged by size: 2042 1310 1035 648 560 454 432 359 292 159 39 MspA1I CmG'CkG Cuts at: 1606 4746 5423 6571 6816 1606 Size: 3140 677 1148 245 2120 Fragments arranged by size: 3140 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2017 2017 Size: 7330 NcoI C'CATG_G Cuts at: 513 1065 2004 2533 3549 513 Size: 552 939 529 1016 4294 Fragments arranged by size: 4294 1016 939 552 529 NdeI CA'TA_TG Cuts at: 387 2740 387 Size: 2353 4977 NgoMIV G'CCGG_C Cuts at: 2015 2015 Size: 7330 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7330 NotI GC'GGCC_GC Cuts at: 4777 4777 Size: 7330 NsiI A_TGCA'T Cuts at: 2173 2740 2173 Size: 567 6763 NspI r_CATG'y Cuts at: 1132 1229 2702 3235 3857 3880 4688 6233 1132 Size: 97 1473 533 622 23 808 1545 2229 Fragments arranged by size: 2229 1545 1473 808 622 533 97 23 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7330 PciI A'CATG_T Cuts at: 2698 3231 6229 2698 Size: 533 2998 3799 Fragments arranged by size: 3799 2998 533 PflMI CCAn_nnn'nTGG Cuts at: 1108 3967 1108 Size: 2859 4471 PmeI GTTT'AAAC Cuts at: 4634 4634 Size: 7330 PmlI CAC'GTG Cuts at: 4507 4507 Size: 7330 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5399 5431 6912 6944 5399 Size: 32 1481 32 5785 Fragments arranged by size: 5785 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7330 PsiI TTA'TAA Cuts at: 4220 4900 4220 Size: 680 6650 PstI C_TGCA'G Cuts at: 830 4544 4682 830 Size: 3714 138 3478 Fragments arranged by size: 3714 3478 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7330 SalI G'TCGA_C Cuts at: 1936 4672 1936 Size: 2736 4594 SbfI CC_TGCA'GG Cuts at: 4682 4682 Size: 7330 ScaI AGT'ACT Cuts at: 5578 7290 5578 Size: 1712 5618 SfcI C'TryA_G Cuts at: 826 1045 3623 4170 4540 4678 5813 6494 6685 Size: 219 2578 547 370 138 1135 681 191 Cuts at: 6685 826 Size: 1471 Fragments arranged by size: 2578 1471 1135 681 547 370 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7330 SmaI CCC'GGG Cuts at: 1872 4657 1872 Size: 2785 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2452 5434 6335 6597 820 Size: 197 926 509 2982 901 262 1553 Fragments arranged by size: 2982 1553 926 901 509 262 197 SnaBI TAC'GTA Cuts at: 493 5121 493 Size: 4628 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7330 SphI G_CATG'C Cuts at: 1132 4688 1132 Size: 3556 3774 SspI AAT'ATT Cuts at: 5 52 5254 5 Size: 47 5202 2081 Fragments arranged by size: 5202 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7330 StyI C'CwwG_G Cuts at: 513 1065 2004 2420 2533 3549 4769 513 Size: 552 939 416 113 1016 1220 3074 Fragments arranged by size: 3074 1220 1016 939 552 416 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2080 5357 5374 5527 5712 6157 325 Size: 1255 500 3277 17 153 185 445 1498 Fragments arranged by size: 3277 1498 1255 500 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3362 3725 4185 Size: 274 80 33 51 1443 1385 363 460 Cuts at: 4185 5576 7288 96 Size: 1391 1712 138 Fragments arranged by size: 1712 1443 1391 1385 460 363 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3258 3290 3790 3822 7290 7322 3258 Size: 32 500 32 3468 32 3266 Fragments arranged by size: 3468 3266 500 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2090 1316 Size: 774 6556 XbaI T'CTAG_A Cuts at: 4666 4666 Size: 7330 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2158 3115 2158 Size: 957 6373 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7330 XmaI C'CCGG_G Cuts at: 1870 4655 1870 Size: 2785 4545 XmnI GAAnn'nnTTC Cuts at: 3474 5459 3474 Size: 1985 5345 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5138 276 Size: 53 83 186 936 3604 2468 Fragments arranged by size: 3604 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FspAI KasI NarI NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI