Restriction map for
pFN21AE0333
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CelII CfoI
ClaI CpoI DraIII DrdI Eam1105I
Eco47III EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 379 GTCTAC GT^[AC][GT]AC
AocI 840 CCTCAGG CC^T[ACGT]AGG
AseI 1839 ATTAAT AT^TAAT
AsnI 1839 ATTAAT AT^TAAT
BalI 122 TGGCCA TGG^CCA
BamHI 771 GGATCC G^GATCC
BbsI 1375 GTCTTC GAAGAC(2/6)
BfaI 736 CTAG C^TAG
BpuAI 1375 GTCTTC GAAGAC(2/6)
BsaBI 1120 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1705 GAGGAG GAGGAG(10/8)
BsiEI 974 CGGCCG CG[GA][CT]^CG
BsmFI 1245 GGGAC GGGAC(10/14)
BspHI 1680 TCATGA T^CATGA
BsrDI 1089 CATTGC GCAATG(2/0)
BsrFI 710 ACCGGC [GA]^CCGG[CT]
BstXI 14 CCAAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 840 CCTCAGG CC^T[ACGT]AGG
Cfr10I 710 ACCGGC [GA]^CCGG[CT]
DraII 410 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 206 CCACGG C^C[GA][CT]GG
EagI 974 CGGCCG C^GGCCG
EarI 1195 GAAGAG CTCTTC(1/4)
EclXI 974 CGGCCG C^GGCCG
Eco52I 974 CGGCCG C^GGCCG
Eco81I 840 CCTCAGG CC^T[ACGT]AGG
EcoO109I 410 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1132 GAATTC G^AATTC
HapII 711 CCGG C^CGG
HpaII 711 CCGG C^CGG
Ksp632I 1195 GAAGAG CTCTTC(1/4)
MaeI 736 CTAG C^TAG
MamI 1120 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 122 TGGCCA TGG^CCA
MscI 122 TGGCCA TGG^CCA
MslI 231 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 711 CCGG C^CGG
NspI 1065 GCATGC [GA]CATG^[CT]
PshBI 1839 ATTAAT AT^TAAT
RcaI 1680 TCATGA T^CATGA
SmlI 1553 CTCAAG C^T[CT][GA]AG
SphI 1065 GCATGC GCATG^C
StuI 475 AGGCCT AGG^CCT
XcmI 520 CCAGACTTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 973 GCGG CCGC(-3/-1)
1069 GCGG
AcsI 778 AAATTC [GA]^AATT[CT]
1132 GAATTC
ApoI 778 AAATTC [GA]^AATT[CT]
1132 GAATTC
Asp700I 693 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1140 GAAGAAGTTC
BsmI 603 GAATGC GAATGC(1/-1)
1713 GAATGC
BstEII 319 GGTGACC G^GT[ACGT]ACC
571 GGTCACC
BstPI 319 GGTGACC G^GT[ACGT]ACC
571 GGTCACC
BstYI 341 GGATCT [GA]^GATC[CT]
771 GGATCC
EcoO65I 319 GGTGACC G^GT[ACGT]ACC
571 GGTCACC
MaeII 1061 ACGT A^CGT
1737 ACGT
MflI 341 GGATCT [GA]^GATC[CT]
771 GGATCC
MwoI 1044 GCTCCAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1056 GCTGGACGTGC
PstI 1186 CTGCAG CTGCA^G
1600 CTGCAG
PvuII 421 CAGCTG CAG^CTG
1490 CAGCTG
SspI 742 AATATT AAT^ATT
1818 AATATT
XhoII 341 GGATCT [GA]^GATC[CT]
771 GGATCC
XmnI 693 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1140 GAAGAAGTTC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AfaI AluI AlwI AlwNI AspHI
AvaII BanII BbvI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EaeI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI NdeII NlaIII NlaIV
PflMI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I