(Circular) (Six-base) MAPSORT of: pFN21AE0333.seq Check: 5901 from: 1 to: 6604 pFN21AE0333 6604 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4414 278 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2385 3947 1937 Size: 448 1562 4594 Fragments arranged by size: 4594 1562 448 Acc65I G'GTAC_C Cuts at: 3925 3925 Size: 6604 AclI AA'CG_TT Cuts at: 1563 4731 5104 1563 Size: 3168 373 3063 Fragments arranged by size: 3168 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 3614 4667 6051 6319 1632 Size: 953 1029 1053 1384 268 1917 Fragments arranged by size: 1917 1384 1053 1029 953 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6407 AflIII A'CryG_T Cuts at: 1203 5503 6480 1203 Size: 4300 977 1327 Fragments arranged by size: 4300 1327 977 AgeI A'CCGG_T Cuts at: 5435 5435 Size: 6604 AhdI GACnn_n'nnGTC Cuts at: 5333 5333 Size: 6604 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6604 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6604 AlwNI CAG_nnn'CTG Cuts at: 2419 2867 3058 4347 5919 2419 Size: 448 191 1289 1572 3104 Fragments arranged by size: 3104 1572 1289 448 191 ApaLI G'TGCA_C Cuts at: 4661 5817 4661 Size: 1156 5448 ApoI r'AATT_y Cuts at: 1493 2783 3137 3913 4139 4291 6400 1493 Size: 1290 354 776 226 152 2109 1697 Fragments arranged by size: 2109 1697 1290 776 354 226 152 AseI AT'TA_AT Cuts at: 160 1032 3845 5158 160 Size: 872 2813 1313 1606 Fragments arranged by size: 2813 1606 1313 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 AvaI C'yCGr_G Cuts at: 1870 1943 3929 1870 Size: 73 1986 4545 Fragments arranged by size: 4545 1986 73 BamHI G'GATC_C Cuts at: 2776 3934 4301 2776 Size: 1158 367 5079 Fragments arranged by size: 5079 1158 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3925 5380 618 Size: 325 217 627 2138 1455 1842 Fragments arranged by size: 2138 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2210 2448 3243 3875 3923 1391 Size: 819 238 795 632 48 4072 Fragments arranged by size: 4072 819 795 632 238 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3373 928 Size: 971 1474 4159 Fragments arranged by size: 4159 1474 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4795 4829 1903 Size: 34 2858 34 3678 Fragments arranged by size: 3678 2858 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4496 5706 4496 Size: 1210 5394 BclI T'GATC_A Cuts at: 1548 1548 Size: 6604 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5215 136 Size: 107 122 71 631 605 3543 1525 Fragments arranged by size: 3543 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6599 6599 Size: 6604 BlpI GC'TnA_GC Cuts at: 4021 4021 Size: 6604 Bme1580I G_kGCm'C Cuts at: 1790 2256 3300 4665 5821 1790 Size: 466 1044 1365 1156 2573 Fragments arranged by size: 2573 1365 1156 1044 466 BmgBI CAC'GTC Cuts at: 1318 3067 1318 Size: 1749 4855 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2877 4335 5293 Size: 416 53 83 399 24 1460 1458 958 Cuts at: 5293 442 Size: 1753 Fragments arranged by size: 1753 1460 1458 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6604 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2441 2579 2624 2957 3035 3312 3416 5264 Size: 1202 138 45 333 78 277 104 1848 Cuts at: 5264 1239 Size: 2579 Fragments arranged by size: 2579 1848 1202 333 277 138 104 78 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3543 4729 5594 5892 3543 Size: 1186 865 298 4255 Fragments arranged by size: 4255 1186 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5267 882 Size: 617 3768 2219 Fragments arranged by size: 3768 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4395 493 Size: 711 3191 2702 Fragments arranged by size: 3191 2702 711 BsaBI GATnn'nnATC Cuts at: 3129 6598 3129 Size: 3469 3135 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4411 4793 5428 275 Size: 53 83 186 936 2878 382 635 1451 Fragments arranged by size: 2878 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5037 5435 5709 5856 6267 5037 Size: 398 274 147 411 5374 Fragments arranged by size: 5374 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 1178 Size: 30 165 30 1149 30 5200 Fragments arranged by size: 5200 1149 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3725 1186 Size: 2539 4065 BseYI C'CCAG_C Cuts at: 2961 3021 5807 6303 2961 Size: 60 2786 496 3262 Fragments arranged by size: 3262 2786 496 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2392 3005 1163 Size: 573 501 155 613 4762 Fragments arranged by size: 4762 613 573 501 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2982 4054 4815 5843 1540 Size: 275 186 981 1072 761 1028 2301 Fragments arranged by size: 2301 1072 1028 981 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2367 2402 2448 3114 3243 3657 3875 4665 4750 Size: 35 46 666 129 414 218 790 85 Cuts at: 4750 5821 2367 Size: 1071 3150 Fragments arranged by size: 3150 1071 790 666 414 218 129 85 46 35 BsmI GAATG_Cn' Cuts at: 2614 3724 4115 4208 2614 Size: 1110 391 93 5010 Fragments arranged by size: 5010 1110 391 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2256 2367 2402 2448 3114 3243 Size: 399 420 46 111 35 46 666 129 Cuts at: 3243 3300 3657 3875 3923 4665 4750 5821 1391 Size: 57 357 218 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 666 420 399 357 218 129 111 85 57 48 46 46 35 BspEI T'CCGG_A Cuts at: 6267 6267 Size: 6604 BspHI T'CATG_A Cuts at: 3685 4493 6223 3685 Size: 808 1730 4066 Fragments arranged by size: 4066 1730 808 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3959 844 Size: 3115 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4491 1394 Size: 429 2668 3507 Fragments arranged by size: 3507 2668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3093 5099 5273 65 Size: 1183 1845 2006 174 1396 Fragments arranged by size: 2006 1845 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2715 5248 5435 1700 Size: 110 905 2533 187 2869 Fragments arranged by size: 2869 2533 905 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6604 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6604 BssSI C'ACGA_G Cuts at: 4664 5676 4664 Size: 1012 5592 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6604 BstBI TT'CG_AA Cuts at: 5408 5408 Size: 6604 BstEII G'GTnAC_C Cuts at: 1184 2324 2576 1184 Size: 1140 252 5212 Fragments arranged by size: 5212 1140 252 BstXI CCAn_nnnn'nTGG Cuts at: 2026 2026 Size: 6604 BstYI r'GATC_y Cuts at: 1972 2346 2776 3934 4301 4686 4703 6155 6241 Size: 374 430 1158 367 385 17 1452 86 Cuts at: 6241 6253 6599 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1158 430 385 374 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2846 2846 Size: 6604 BtgI C'CryG_G Cuts at: 513 1065 2004 2211 513 Size: 552 939 207 4906 Fragments arranged by size: 4906 939 552 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2325 487 Size: 683 56 1099 4766 Fragments arranged by size: 4766 1099 683 56 BtsI GCAGTG_nn' Cuts at: 2898 2931 2952 3477 4120 4914 4934 2898 Size: 33 21 525 643 794 20 4568 Fragments arranged by size: 4568 794 643 525 33 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6604 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2491 2526 529 Size: 35 1927 35 4607 Fragments arranged by size: 4607 1927 35 35 DraI TTT'AAA Cuts at: 3519 3739 3883 3908 3519 Size: 220 144 25 6215 Fragments arranged by size: 6215 220 144 25 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5611 809 Size: 507 4295 1802 Fragments arranged by size: 4295 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2717 2979 Size: 54 1425 50 275 18 297 590 262 Cuts at: 2979 4051 4940 6569 8 Size: 1072 889 1629 43 Fragments arranged by size: 1629 1425 1072 889 590 297 275 262 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2979 4051 1537 Size: 275 1167 1072 4090 Fragments arranged by size: 4090 1167 1072 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3195 4534 1353 Size: 1842 1339 3423 Fragments arranged by size: 3423 1842 1339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5191 5565 5711 387 Size: 1064 138 3602 374 146 1280 Fragments arranged by size: 3602 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2446 3241 3873 2446 Size: 795 632 5177 Fragments arranged by size: 5177 795 632 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2441 2579 2585 2624 2957 3035 3312 Size: 393 809 138 6 39 333 78 277 Cuts at: 3312 3416 3614 4667 5264 6051 6319 1239 Size: 104 198 1053 597 787 268 1524 Fragments arranged by size: 1524 1053 809 787 597 393 333 277 268 198 138 104 78 39 6 EcoNI CCTnn'n_nnAGG Cuts at: 2476 2849 3082 2476 Size: 373 233 5998 Fragments arranged by size: 5998 373 233 EcoO109I rG'GnC_Cy Cuts at: 2416 2416 Size: 6604 EcoRI G'AATT_C Cuts at: 3137 3913 4291 3137 Size: 776 378 5450 Fragments arranged by size: 5450 776 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3916 3916 Size: 6604 FspI TGC'GCA Cuts at: 5110 5110 Size: 6604 HaeII r_GCGC'y Cuts at: 1678 5751 1678 Size: 4073 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2158 2190 2744 2776 3541 Size: 32 135 32 586 32 554 32 765 Cuts at: 3541 3573 5245 5277 5319 5351 1373 Size: 32 1672 32 42 32 2626 Fragments arranged by size: 2626 1672 765 586 554 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3948 4194 669 Size: 988 281 2010 246 3079 Fragments arranged by size: 3079 2010 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6604 HpaI GTT'AAC Cuts at: 4194 4194 Size: 6604 KpnI G_GTAC'C Cuts at: 3929 3929 Size: 6604 MfeI C'AATT_G Cuts at: 4203 4203 Size: 6604 MluI A'CGCG_T Cuts at: 6480 6480 Size: 6604 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3630 5718 5902 1305 Size: 2325 2088 184 2007 Fragments arranged by size: 2325 2088 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2129 10 Size: 54 1425 343 297 4485 Fragments arranged by size: 4485 1425 343 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 4562 4921 5080 518 Size: 648 1074 2322 359 159 2042 Fragments arranged by size: 2322 2042 1074 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2428 2978 3497 4020 4697 5845 6090 1606 Size: 822 550 519 523 677 1148 245 2120 Fragments arranged by size: 2120 1148 822 677 550 523 519 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5113 Fragments arranged by size: 5113 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6604 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6604 NotI GC'GGCC_GC Cuts at: 4051 4051 Size: 6604 NspI r_CATG'y Cuts at: 1132 1229 3074 3962 5507 1132 Size: 97 1845 888 1545 2229 Fragments arranged by size: 2229 1845 1545 888 97 PasI CC'CwG_GG Cuts at: 1348 2664 1348 Size: 1316 5288 PciI A'CATG_T Cuts at: 5503 5503 Size: 6604 PflMI CCAn_nnn'nTGG Cuts at: 1108 2281 2419 3016 1108 Size: 1173 138 597 4696 Fragments arranged by size: 4696 1173 597 138 PfoI T'CCnGG_A Cuts at: 2342 2787 3051 3289 3393 2342 Size: 445 264 238 104 5553 Fragments arranged by size: 5553 445 264 238 104 PmeI GTTT'AAAC Cuts at: 3908 3908 Size: 6604 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4673 4705 6186 6218 4673 Size: 32 1481 32 5059 Fragments arranged by size: 5059 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6604 PsiI TTA'TAA Cuts at: 4174 4174 Size: 6604 PstI C_TGCA'G Cuts at: 830 3195 3609 3956 830 Size: 2365 414 347 3478 Fragments arranged by size: 3478 2365 414 347 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6604 PvuII CAG'CTG Cuts at: 2428 3497 2428 Size: 1069 5535 SacI G_AGCT'C Cuts at: 2448 3243 3875 2448 Size: 795 632 5177 Fragments arranged by size: 5177 795 632 SalI G'TCGA_C Cuts at: 1936 3946 1936 Size: 2010 4594 SbfI CC_TGCA'GG Cuts at: 3956 3956 Size: 6604 ScaI AGT'ACT Cuts at: 4852 6564 4852 Size: 1712 4892 SfcI C'TryA_G Cuts at: 826 1045 3004 3191 3605 3952 5087 5768 5959 Size: 219 1959 187 414 347 1135 681 191 Cuts at: 5959 826 Size: 1471 Fragments arranged by size: 1959 1471 1135 681 414 347 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6604 SmaI CCC'GGG Cuts at: 1872 3931 1872 Size: 2059 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3558 4708 5609 5871 820 Size: 197 926 1615 1150 901 262 1553 Fragments arranged by size: 1615 1553 1150 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4395 493 Size: 3902 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6604 SphI G_CATG'C Cuts at: 1132 3074 3962 1132 Size: 1942 888 3774 Fragments arranged by size: 3774 1942 888 SspI AAT'ATT Cuts at: 5 52 2749 3825 4528 5 Size: 47 2697 1076 703 2081 Fragments arranged by size: 2697 2081 1076 703 47 StuI AGG'CCT Cuts at: 1517 2482 1517 Size: 965 5639 StyI C'CwwG_G Cuts at: 513 1065 2004 2229 2477 3792 4043 513 Size: 552 939 225 248 1315 251 3074 Fragments arranged by size: 3074 1315 939 552 251 248 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2274 4631 4648 4801 4986 5431 325 Size: 1255 694 2357 17 153 185 445 1498 Fragments arranged by size: 2357 1498 1255 694 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4850 6562 96 Size: 274 80 33 51 1443 2873 1712 138 Fragments arranged by size: 2873 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6564 6596 6564 Size: 32 6572 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6604 XbaI T'CTAG_A Cuts at: 3940 3940 Size: 6604 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2532 2532 Size: 6604 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6604 XmaI C'CCGG_G Cuts at: 1870 3929 1870 Size: 2059 4545 XmnI GAAnn'nnTTC Cuts at: 2702 3149 4733 2702 Size: 447 1584 4573 Fragments arranged by size: 4573 1584 447 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4412 276 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI