Restriction map for
pFN21AE0333
(Linear) (Six-base) MAPSORT of: KIEE0333.seq Check: 9056 from: 1 to: 1899
KIEE0333.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 380 1899
Size: 380 1519
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1609 1899
Size: 580 1029 290
Fragments arranged by size:
1029 580 290
AlwNI CAG_nnn'CTG
Cuts at: 0 414 862 1053 1899
Size: 414 448 191 846
Fragments arranged by size:
846 448 414 191
ApoI r'AATT_y
Cuts at: 0 778 1132 1899
Size: 778 354 767
Fragments arranged by size:
778 767 354
AseI AT'TA_AT
Cuts at: 0 1840 1899
Size: 1840 59
BamHI G'GATC_C
Cuts at: 0 771 1899
Size: 771 1128
BanII G_rGCy'C
Cuts at: 0 205 443 1238 1870 1899
Size: 205 238 795 632 29
Fragments arranged by size:
795 632 238 205 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1368 1899
Size: 1368 531
Bme1580I G_kGCm'C
Cuts at: 0 251 1295 1899
Size: 251 1044 604
Fragments arranged by size:
1044 604 251
BmgBI CAC'GTC
Cuts at: 0 1062 1899
Size: 1062 837
BmrI ACTGGGnnnn_n'
Cuts at: 0 872 1899
Size: 872 1027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 574 619 952 1030 1307 1411 1899
Size: 436 138 45 333 78 277 104 488
Fragments arranged by size:
488 436 333 277 138 104 78 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1538 1899
Size: 1538 361
BsaBI GATnn'nnATC
Cuts at: 0 1124 1899
Size: 1124 775
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 547 577 1899
Size: 547 30 1322
Fragments arranged by size:
1322 547 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1720 1899
Size: 1720 179
BseYI C'CCAG_C
Cuts at: 0 956 1016 1899
Size: 956 60 883
Fragments arranged by size:
956 883 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 387 1000 1899
Size: 232 155 613 899
Fragments arranged by size:
899 613 232 155
BsiEI CG_ry'CG
Cuts at: 0 977 1899
Size: 977 922
BsiHKAI G_wGCw'C
Cuts at: 0 362 397 443 1109 1238 1652 1870 1899
Size: 362 35 46 666 129 414 218 29
Fragments arranged by size:
666 414 362 218 129 46 35 29
BsmI GAATG_Cn'
Cuts at: 0 609 1719 1899
Size: 609 1110 180
Fragments arranged by size:
1110 609 180
Bsp1286I G_dGCh'C
Cuts at: 0 205 251 362 397 443 1109 1238 1295
Size: 205 46 111 35 46 666 129 57
Cuts at: 1295 1652 1870 1899
Size: 357 218 29
Fragments arranged by size:
666 357 218 205 129 111 57 46
46 35 29
BspHI T'CATG_A
Cuts at: 0 1680 1899
Size: 1680 219
BsrDI GCAATG_nn'
Cuts at: 0 1088 1899
Size: 1088 811
BsrFI r'CCGG_y
Cuts at: 0 710 1899
Size: 710 1189
BstEII G'GTnAC_C
Cuts at: 0 319 571 1899
Size: 319 252 1328
Fragments arranged by size:
1328 319 252
BstXI CCAn_nnnn'nTGG
Cuts at: 0 21 1899
Size: 21 1878
BstYI r'GATC_y
Cuts at: 0 341 771 1899
Size: 341 430 1128
Fragments arranged by size:
1128 430 341
Bsu36I CC'TnA_GG
Cuts at: 0 841 1899
Size: 841 1058
BtgI C'CryG_G
Cuts at: 0 206 1899
Size: 206 1693
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 320 1899
Size: 320 1579
BtsI GCAGTG_nn'
Cuts at: 0 893 926 947 1472 1899
Size: 893 33 21 525 427
Fragments arranged by size:
893 525 427 33 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 486 521 1899
Size: 486 35 1378
Fragments arranged by size:
1378 486 35
DraI TTT'AAA
Cuts at: 0 1514 1734 1878 1899
Size: 1514 220 144 21
Fragments arranged by size:
1514 220 144 21
EaeI y'GGCC_r
Cuts at: 0 122 712 974 1899
Size: 122 590 262 925
Fragments arranged by size:
925 590 262 122
EagI C'GGCC_G
Cuts at: 0 974 1899
Size: 974 925
EarI CTCTTCn'nnn_
Cuts at: 0 1190 1899
Size: 1190 709
EcoICRI GAG'CTC
Cuts at: 0 441 1236 1868 1899
Size: 441 795 632 31
Fragments arranged by size:
795 632 441 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 574 580 619 952 1030 1307 1411
Size: 436 138 6 39 333 78 277 104
Cuts at: 1411 1609 1899
Size: 198 290
Fragments arranged by size:
436 333 290 277 198 138 104 78
39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 471 844 1077 1899
Size: 471 373 233 822
Fragments arranged by size:
822 471 373 233
EcoO109I rG'GnC_Cy
Cuts at: 0 411 1899
Size: 411 1488
EcoRI G'AATT_C
Cuts at: 0 1132 1899
Size: 1132 767
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 739 771 1536 1568 1899
Size: 153 32 554 32 765 32 331
Fragments arranged by size:
765 554 331 153 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1625 1899
Size: 1625 274
MscI TGG'CCA
Cuts at: 0 124 1899
Size: 124 1775
MslI CAynn'nnrTG
Cuts at: 0 235 1899
Size: 235 1664
MspA1I CmG'CkG
Cuts at: 0 423 973 1492 1899
Size: 423 550 519 407
Fragments arranged by size:
550 519 423 407
NspI r_CATG'y
Cuts at: 0 1069 1899
Size: 1069 830
PasI CC'CwG_GG
Cuts at: 0 659 1899
Size: 659 1240
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 414 1011 1899
Size: 276 138 597 888
Fragments arranged by size:
888 597 276 138
PfoI T'CCnGG_A
Cuts at: 0 337 782 1046 1284 1388 1899
Size: 337 445 264 238 104 511
Fragments arranged by size:
511 445 337 264 238 104
PstI C_TGCA'G
Cuts at: 0 1190 1604 1899
Size: 1190 414 295
Fragments arranged by size:
1190 414 295
PvuII CAG'CTG
Cuts at: 0 423 1492 1899
Size: 423 1069 407
Fragments arranged by size:
1069 423 407
SacI G_AGCT'C
Cuts at: 0 443 1238 1870 1899
Size: 443 795 632 29
Fragments arranged by size:
795 632 443 29
SfcI C'TryA_G
Cuts at: 0 999 1186 1600 1899
Size: 999 187 414 299
Fragments arranged by size:
999 414 299 187
SmlI C'TyrA_G
Cuts at: 0 1553 1899
Size: 1553 346
SphI G_CATG'C
Cuts at: 0 1069 1899
Size: 1069 830
SspI AAT'ATT
Cuts at: 0 744 1820 1899
Size: 744 1076 79
Fragments arranged by size:
1076 744 79
StuI AGG'CCT
Cuts at: 0 477 1899
Size: 477 1422
StyI C'CwwG_G
Cuts at: 0 224 472 1787 1899
Size: 224 248 1315 112
Fragments arranged by size:
1315 248 224 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 269 1899
Size: 269 1630
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 527 1899
Size: 527 1372
XmnI GAAnn'nnTTC
Cuts at: 0 697 1144 1899
Size: 697 447 755
Fragments arranged by size:
755 697 447
Enzymes that do cut:
AccI AcuI AlwNI ApoI AseI BamHI BanII BbsI
Bme1580I BmgBI BmrI BpmI BpuEI BsaBI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI
BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI Hin4I MmeI MscI MslI MspA1I NspI
PasI PflMI PfoI PstI PvuII SacI SfcI SmlI
SphI SspI StuI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I
BsaI BsaAI BsaHI BsaWI BsiWI BsmBI BspEI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I ClaI
DraIII DrdI EciI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TatI TstI
Tth111I XbaI XhoI XmaI ZraI