(Circular) (Six-base) MAPSORT of: pFN21AE0332.seq Check: 1550 from: 1 to: 6583 pFN21AE0332 6583 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2035 2944 2035 Size: 909 5674 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4393 278 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3926 1937 Size: 1989 4594 Acc65I G'GTAC_C Cuts at: 3904 3904 Size: 6583 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4646 6030 6298 1632 Size: 3014 1384 268 1917 Fragments arranged by size: 3014 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 2096 3149 5482 6459 1203 Size: 893 1053 2333 977 1327 Fragments arranged by size: 2333 1327 1053 977 893 AgeI A'CCGG_T Cuts at: 2214 5414 2214 Size: 3200 3383 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6583 AlwNI CAG_nnn'CTG Cuts at: 2050 3446 4326 5898 2050 Size: 1396 880 1572 2735 Fragments arranged by size: 2735 1572 1396 880 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 3472 3892 4118 4270 6379 1493 Size: 1979 420 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 420 226 152 AseI AT'TA_AT Cuts at: 160 1032 2834 5137 160 Size: 872 1802 2303 1606 Fragments arranged by size: 2303 1802 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 3908 1870 Size: 73 1965 4545 Fragments arranged by size: 4545 1965 73 AvrII C'CTAG_G Cuts at: 2380 2380 Size: 6583 BamHI G'GATC_C Cuts at: 2076 3913 4280 2076 Size: 1837 367 4379 Fragments arranged by size: 4379 1837 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3904 5359 618 Size: 325 217 627 2117 1455 1842 Fragments arranged by size: 2117 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3902 1391 Size: 2511 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2042 2542 2895 928 Size: 971 143 500 353 4616 Fragments arranged by size: 4616 971 500 353 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4774 4808 1903 Size: 34 2837 34 3678 Fragments arranged by size: 3678 2837 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3283 4475 5685 3283 Size: 1192 1210 4181 Fragments arranged by size: 4181 1210 1192 BclI T'GATC_A Cuts at: 1548 1548 Size: 6583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5194 136 Size: 107 122 71 631 605 337 3185 1525 Fragments arranged by size: 3185 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 4000 4000 Size: 6583 Bme1580I G_kGCm'C Cuts at: 1790 4644 5800 1790 Size: 2854 1156 2573 Fragments arranged by size: 2854 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2032 2124 2365 2557 1318 Size: 714 92 241 192 5344 Fragments arranged by size: 5344 714 241 192 92 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3008 4314 5272 Size: 416 53 83 399 24 1591 1306 958 Cuts at: 5272 442 Size: 1753 Fragments arranged by size: 1753 1591 1306 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3566 Size: 6583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2212 3300 3329 3561 3640 3730 5243 1239 Size: 973 1088 29 232 79 90 1513 2579 Fragments arranged by size: 2579 1513 1088 973 232 90 79 29 Bpu10I CC'TnA_GC Cuts at: 3084 3084 Size: 6583 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2612 3219 4708 5573 5871 2612 Size: 607 1489 865 298 3324 Fragments arranged by size: 3324 1489 865 607 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5246 882 Size: 617 3747 2219 Fragments arranged by size: 3747 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2097 3152 4374 493 Size: 711 893 1055 1222 2702 Fragments arranged by size: 2702 1222 1055 893 711 BsaBI GATnn'nnATC Cuts at: 6577 6577 Size: 6583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 4390 4772 5407 Size: 53 83 186 936 486 2371 382 635 Cuts at: 5407 275 Size: 1451 Fragments arranged by size: 2371 1451 936 635 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2214 5016 5414 5688 5835 6246 2214 Size: 2802 398 274 147 411 2551 Fragments arranged by size: 2802 2551 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2135 2165 2663 2693 3528 Size: 30 165 30 732 30 498 30 835 Cuts at: 3528 3558 3714 3744 1178 Size: 30 156 30 4017 Fragments arranged by size: 4017 835 732 498 165 156 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6583 BseYI C'CCAG_C Cuts at: 5786 6282 5786 Size: 496 6087 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 2157 2568 3756 1163 Size: 573 327 94 411 1188 3990 Fragments arranged by size: 3990 1188 573 411 327 94 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2215 3679 4033 4794 5822 1540 Size: 275 186 214 1464 354 761 1028 2301 Fragments arranged by size: 2301 1464 1028 761 354 275 214 186 BsiHKAI G_wGCw'C Cuts at: 4644 4729 5800 4644 Size: 85 1071 5427 Fragments arranged by size: 5427 1071 85 BsmI GAATG_Cn' Cuts at: 3203 3333 4094 4187 3203 Size: 130 761 93 5599 Fragments arranged by size: 5599 761 130 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3902 4644 4729 5800 1391 Size: 399 2112 742 85 1071 2174 Fragments arranged by size: 2174 2112 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6246 6246 Size: 6583 BspHI T'CATG_A Cuts at: 4472 6202 4472 Size: 1730 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2035 2944 3938 844 Size: 1191 909 994 3489 Fragments arranged by size: 3489 1191 994 909 BsrBI CCG'CTC Cuts at: 1394 1823 4470 1394 Size: 429 2647 3507 Fragments arranged by size: 3507 2647 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3162 5078 5252 65 Size: 1183 1914 1916 174 1396 Fragments arranged by size: 1916 1914 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2214 5227 5414 1700 Size: 110 404 3013 187 2869 Fragments arranged by size: 3013 2869 404 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6583 BssSI C'ACGA_G Cuts at: 2540 4643 5655 2540 Size: 2103 1012 3468 Fragments arranged by size: 3468 2103 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2050 794 Size: 1256 5327 BstBI TT'CG_AA Cuts at: 3427 5387 3427 Size: 1960 4623 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6583 BstYI r'GATC_y Cuts at: 1972 2076 3313 3913 4280 4665 4682 6134 6220 Size: 104 1237 600 367 385 17 1452 86 Cuts at: 6220 6232 6578 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1237 600 385 367 346 104 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2306 2306 Size: 6583 BtgI C'CryG_G Cuts at: 513 1065 3505 513 Size: 552 2440 3591 Fragments arranged by size: 3591 2440 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5844 Fragments arranged by size: 5844 683 56 BtsI GCAGTG_nn' Cuts at: 2140 3786 4099 4893 4913 2140 Size: 1646 313 794 20 3810 Fragments arranged by size: 3810 1646 794 313 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3887 3887 Size: 6583 DraIII CAC_nnn'GTG Cuts at: 2127 2127 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2113 2659 Size: 54 1425 50 275 18 180 103 546 Cuts at: 2659 3484 3676 4030 4919 6548 8 Size: 825 192 354 889 1629 43 Fragments arranged by size: 1629 1425 889 825 546 354 275 192 180 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3676 4030 1537 Size: 275 1864 354 4090 Fragments arranged by size: 4090 1864 354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 4513 1353 Size: 812 2348 3423 Fragments arranged by size: 3423 2348 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3491 5170 5544 5690 387 Size: 1064 138 1902 1679 374 146 1280 Fragments arranged by size: 1902 1679 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 3300 3329 3561 3640 3730 4646 Size: 393 580 1088 29 232 79 90 916 Cuts at: 4646 5243 6030 6298 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1088 916 787 597 580 393 268 232 90 79 29 EcoNI CCTnn'n_nnAGG Cuts at: 2384 2435 2384 Size: 51 6532 EcoO109I rG'GnC_Cy Cuts at: 2376 2376 Size: 6583 EcoRI G'AATT_C Cuts at: 3892 4270 3892 Size: 378 6205 EcoRV GAT'ATC Cuts at: 3760 3760 Size: 6583 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 5730 1678 Size: 4052 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2462 2494 2950 Size: 32 135 32 563 32 295 32 456 Cuts at: 2950 2982 3278 3310 3356 3388 5224 5256 5298 Size: 32 296 32 46 32 1836 32 42 Cuts at: 5298 5330 1373 Size: 32 2626 Fragments arranged by size: 2626 1836 563 456 296 295 135 46 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 3927 4173 669 Size: 988 281 424 1565 246 3079 Fragments arranged by size: 3079 1565 988 424 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KpnI G_GTAC'C Cuts at: 3908 3908 Size: 6583 MfeI C'AATT_G Cuts at: 2823 3340 4182 2823 Size: 517 842 5224 Fragments arranged by size: 5224 842 517 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5697 5881 1305 Size: 4392 184 2007 Fragments arranged by size: 4392 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2012 2115 2661 3486 10 Size: 54 1425 343 180 103 546 825 3107 Fragments arranged by size: 3107 1425 825 546 343 180 103 54 MslI CAynn'nnrTG Cuts at: 518 1166 4541 4900 5059 518 Size: 648 3375 359 159 2042 Fragments arranged by size: 3375 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2141 3740 3999 4676 5824 6069 1606 Size: 535 1599 259 677 1148 245 2120 Fragments arranged by size: 2120 1599 1148 677 535 259 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6031 NdeI CA'TA_TG Cuts at: 387 2977 387 Size: 2590 3993 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 2240 2406 3386 3941 5486 1132 Size: 97 1011 166 980 555 1545 2229 Fragments arranged by size: 2229 1545 1011 980 555 166 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6583 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 2668 1108 Size: 1560 5023 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PmlI CAC'GTG Cuts at: 2097 3152 2097 Size: 1055 5528 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3009 3041 4652 4684 6165 6197 3009 Size: 32 1611 32 1481 32 3395 Fragments arranged by size: 3395 1611 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2376 2376 Size: 6583 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PstI C_TGCA'G Cuts at: 830 2135 3451 3935 830 Size: 1305 1316 484 3478 Fragments arranged by size: 3478 1316 1305 484 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 3740 3740 Size: 6583 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SanDI GG'GwC_CC Cuts at: 2376 2376 Size: 6583 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 4831 6543 4831 Size: 1712 4871 SfcI C'TryA_G Cuts at: 826 1045 2131 2727 3447 3703 3931 5066 5747 Size: 219 1086 596 720 256 228 1135 681 Cuts at: 5747 5938 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1086 720 681 596 256 228 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 3910 1872 Size: 2038 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2591 3234 4687 5588 5850 820 Size: 197 926 648 643 1453 901 262 1553 Fragments arranged by size: 1553 1453 926 901 648 643 262 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 3809 152 Size: 3657 2926 SphI G_CATG'C Cuts at: 1132 3386 3941 1132 Size: 2254 555 3774 Fragments arranged by size: 3774 2254 555 SspI AAT'ATT Cuts at: 5 52 2767 4507 5 Size: 47 2715 1740 2081 Fragments arranged by size: 2715 2081 1740 47 StuI AGG'CCT Cuts at: 1517 2675 1517 Size: 1158 5425 StyI C'CwwG_G Cuts at: 513 1065 2380 3408 3492 4022 513 Size: 552 1315 1028 84 530 3074 Fragments arranged by size: 3074 1315 1028 552 530 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2254 2264 4610 4627 4780 4965 5410 Size: 1255 674 10 2346 17 153 185 445 Cuts at: 5410 325 Size: 1498 Fragments arranged by size: 2346 1498 1255 674 445 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3344 4829 6541 Size: 274 80 33 51 1443 1367 1485 1712 Cuts at: 6541 96 Size: 138 Fragments arranged by size: 1712 1485 1443 1367 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2663 2695 2784 2816 3796 3828 6543 6575 2663 Size: 32 89 32 980 32 2715 32 2671 Fragments arranged by size: 2715 2671 980 89 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6583 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3631 3631 Size: 6583 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6583 XmaI C'CCGG_G Cuts at: 1870 3908 1870 Size: 2038 4545 XmnI GAAnn'nnTTC Cuts at: 4712 4712 Size: 6583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4391 276 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI