################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeIII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AfaI 339 GTAC GT^AC AhdI 478 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 478 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 511 GAGCTC G[AT]GC[AT]^C AvaI 388 CCCGGG C^[CT]CG[GA]G AvaII 477 GGACC G^G[AT]CC BanI 57 GGTGCC G^G[CT][GA]CC BanII 511 GAGCTC G[GA]GC[CT]^C BfaI 469 CTAG C^TAG BlpI 45 GCTGAGC GC^T[ACGT]AGC Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC BsaI 85 GGTCTC GGTCTC(1/5) BseRI 127 CTCCTC GAGGAG(10/8) BsiHKAI 511 GAGCTC G[AT]GC[AT]^C BsmBI 334 GAGACG CGTCTC(1/5) BsoBI 388 CCCGGG C^[CT]CG[GA]G BsrBI 352 CCGCTC CCGCTC(-3/-3) BstF5I 150 CATCC GGATG(2/0) CelII 45 GCTGAGC GC^T[ACGT]AGC CfoI 72 GCGC GCG^C Cfr13I 477 GGACC G^G[ACGT]CC DpnII 189 GATC ^GATC Eam1105I 478 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 309 CCTAACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 68 GCGGG CCCGC(4/6) FokI 150 CATCC GGATG(9/13) HaeII 71 GGCGCT [GA]GCGC^[CT] HapII 389 CCGG C^CGG HgaI 29 GCGTC GACGC(5/10) HhaI 72 GCGC GCG^C HinP1I 72 GCGC G^CGC HpaII 389 CCGG C^CGG HphI 180 TCACC GGTGA(8/7) MaeI 469 CTAG C^TAG MaeIII 454 GTGAC ^GT[ACGT]AC MboI 189 GATC ^GATC MspI 389 CCGG C^CGG NdeII 189 GATC ^GATC NlaIII 133 CATG CATG^ PleI 445 GACTC GAGTC(4/5) PvuII 206 CAGCTG CAG^CTG RsaI 339 GTAC GT^AC SacI 511 GAGCTC GAGCT^C Sau3AI 189 GATC ^GATC Sau96I 477 GGACC G^G[ACGT]CC SmaI 388 CCCGGG CCC^GGG SmlI 355 CTCAAG C^T[CT][GA]AG TaqI 515 TCGA T^CGA Tsp45I 454 GTGAC ^GT[GC]AC TspRI 22 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 515 TCGA T^CGA XcmI 497 CCACAACGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 388 CCCGGG C^CCGGG XmaI 388 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 33 CGCG CG^CG 54 CGCG BbvI 3 GCAGC GCAGC(8/12) 348 GCAGC BcnI 388 CCCGG CC^[GC]GG 389 CCGGG BmyI 58 GTGCCC G[AGT]GC[ACT]^C 511 GAGCTC BpmI 449 CTGGAG CTGGAG(16/14) 508 CTGGAG BsmFI 476 GGGAC GGGAC(10/14) 234 GTCCC Bsp1286I 58 GTGCCC G[AGT]GC[ACT]^C 511 GAGCTC BsrI 12 ACTGG ACTGG(1/-1) 457 ACTGG BstNI 364 CCTGG CC^[AT]GG 437 CCTGG BstUI 33 CGCG CG^CG 54 CGCG Cac8I 68 GCGGGC GC[ACGT]^[ACGT]GC 383 GCAAGC EcoRII 364 CCTGG ^CC[AT]GG 437 CCTGG MaeII 337 ACGT A^CGT 502 ACGT MboII 369 GAAGA GAAGA(8/7) 173 TCTTC MspA1I 4 CAGCGG C[AC]G^C[GT]G 206 CAGCTG MvaI 364 CCTGG CC^[AT]GG 437 CCTGG MvnI 33 CGCG CG^CG 54 CGCG NciI 388 CCCGG CC^[GC]GG 389 CCGGG NlaIV 57 GGTGCC GG[ACGT]^[ACGT]CC 476 GGGACC TfiI 170 GATTC G^A[AT]TC 517 GATTC TseI 3 GCAGC G^C[AT]GC 348 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AciI AluI BsaJI BsiYI BslI BsmAI BssKI DdeI Fnu4HI HinfI ItaI MnlI ScrFI