################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BssHII BssSI BstAPI BstF5I BstUI BstXI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FokI FseI FspI HapII Hin1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 71 AAATTC [GA]^AATT[CT] Alw44I 368 GTGCAC G^TGCAC AlwI 639 GATCC GGATC(4/5) AlwNI 55 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 368 GTGCAC G^TGCAC ApoI 71 AAATTC [GA]^AATT[CT] AseI 205 ATTAAT AT^TAAT AsnI 205 ATTAAT AT^TAAT AvaII 262 GGTCC G^G[AT]CC BanI 670 GGCACC G^G[CT][GA]CC BciVI 36 GGATAC GTATCC(6/5) BsaJI 624 CCATGG C^C[ACGT][ACGT]GG BseRI 1024 GAGGAG GAGGAG(10/8) Bsp1407I 988 TGTACA T^GTACA BsrBI 16 GAGCGG CCGCTC(-3/-3) BsrGI 988 TGTACA T^GTACA BssKI 536 CCTGG ^CC[ACGT]GG Bst1107I 851 GTATAC GTA^TAC BstEII 979 GGTGACC G^GT[ACGT]ACC BstNI 536 CCTGG CC^[AT]GG BstPI 979 GGTGACC G^GT[ACGT]ACC BstZ17I 851 GTATAC GTA^TAC DsaI 624 CCATGG C^C[GA][CT]GG EcoO65I 979 GGTGACC G^GT[ACGT]ACC EcoRII 536 CCTGG ^CC[AT]GG EcoT14I 624 CCATGG C^C[AT][AT]GG FauI 18 GCGGG CCCGC(4/6) HgaI 45 GCGTC GACGC(5/10) HincII 220 GTTGAC GT[CT]^[GA]AC HindII 220 GTTGAC GT[CT]^[GA]AC MaeII 1053 ACGT A^CGT MspA1I 58 CCGCTG C[AC]G^C[GT]G MvaI 536 CCTGG CC^[AT]GG NcoI 624 CCATGG C^CATGG NdeI 158 CATATG CA^TATG NspI 277 ACATGC [GA]CATG^[CT] PflMI 541 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 688 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 205 ATTAAT AT^TAAT ScaI 374 AGTACT AGT^ACT ScrFI 536 CCTGG CC^[ACGT]GG SfaNI 399 GATGC GCATC(5/9) SfcI 645 CTGTAG C^T[GA][CT]AG SspBI 988 TGTACA T^GTACA StyI 624 CCATGG C^C[AT][AT]GG Tsp45I 980 GTGAC ^GT[GC]AC Van91I 541 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 957 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 685 GTAGAC GT^[AC][GT]AC 851 GTATAC AspHI 368 GTGCAC G[AT]GC[AT]^C 1040 GTGCTC BbsI 312 GAAGAC GAAGAC(2/6) 883 GAAGAC BclI 162 TGATCA T^GATCA 903 TGATCA BfaI 848 CTAG C^TAG 958 CTAG BmyI 368 GTGCAC G[AGT]GC[ACT]^C 1040 GTGCTC BpuAI 312 GAAGAC GAAGAC(2/6) 883 GAAGAC BsgI 650 GTGCAG GTGCAG(16/14) 1093 GTGCAG BsiHKAI 368 GTGCAC G[AT]GC[AT]^C 1040 GTGCTC BsmFI 84 GTCCC GGGAC(10/14) 553 GTCCC Bsp1286I 368 GTGCAC G[AGT]GC[ACT]^C 1040 GTGCTC BsrI 377 ACTGG ACTGG(1/-1) 212 CCAGT BstBI 25 TTCGAA TT^CGAA 74 TTCGAA CfoI 663 GCGC GCG^C 1202 GCGC Cfr13I 262 GGTCC G^G[ACGT]CC 628 GGGCC DraI 406 TTTAAA TTT^AAA 589 TTTAAA EarI 28 GAAGAG CTCTTC(1/4) 229 GAAGAG FbaI 162 TGATCA T^GATCA 903 TGATCA HaeII 662 GGCGCT [GA]GCGC^[CT] 1201 GGCGCT HhaI 663 GCGC GCG^C 1202 GCGC HinP1I 663 GCGC G^CGC 1202 GCGC Ksp632I 28 GAAGAG CTCTTC(1/4) 229 GAAGAG MaeI 848 CTAG C^TAG 958 CTAG NspV 25 TTCGAA TT^CGAA 74 TTCGAA PleI 82 GAGTC GAGTC(4/5) 551 GAGTC Sau96I 262 GGTCC G^G[ACGT]CC 628 GGGCC SfuI 25 TTCGAA TT^CGAA 74 TTCGAA ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AfaI AluI BalI BbvI BsiYI BslI Cac8I DdeI DpnII EaeI Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MwoI NdeII NlaIII NlaIV RsaI Sau3AI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I