(Circular) (Six-base) MAPSORT of: pFN21AE0933.seq Check: 9033 from: 1 to: 5950 pFN21AE0933 5950 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2691 2857 3293 1937 Size: 754 166 436 4594 Fragments arranged by size: 4594 754 436 166 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4013 5397 5665 1632 Size: 2381 1384 268 1917 Fragments arranged by size: 2381 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 4849 5826 1203 Size: 3646 977 1327 Fragments arranged by size: 3646 1327 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 2175 2652 1166 Size: 1009 477 4464 Fragments arranged by size: 4464 1009 477 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 2065 3693 5265 2065 Size: 1628 1572 2750 Fragments arranged by size: 2750 1628 1572 ApaLI G'TGCA_C Cuts at: 2373 4007 5163 2373 Size: 1634 1156 3160 Fragments arranged by size: 3160 1634 1156 ApoI r'AATT_y Cuts at: 1493 2076 3259 3485 3637 5746 1493 Size: 583 1183 226 152 2109 1697 Fragments arranged by size: 2109 1697 1183 583 226 152 AseI AT'TA_AT Cuts at: 160 1032 2211 4504 160 Size: 872 1179 2293 1606 Fragments arranged by size: 2293 1606 1179 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 3275 1870 Size: 73 1332 4545 Fragments arranged by size: 4545 1332 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2675 3271 4726 618 Size: 325 217 627 888 596 1455 1842 Fragments arranged by size: 1842 1455 888 627 596 325 217 BanII G_rGCy'C Cuts at: 1391 3269 1391 Size: 1878 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2324 2895 928 Size: 971 425 571 3983 Fragments arranged by size: 3983 971 571 425 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4141 4175 1903 Size: 34 50 34 2120 34 3678 Fragments arranged by size: 3678 2120 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2035 3842 5052 2035 Size: 1807 1210 2933 Fragments arranged by size: 2933 1807 1210 BclI T'GATC_A Cuts at: 1548 2167 2908 1548 Size: 619 741 4590 Fragments arranged by size: 4590 741 619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4561 136 Size: 107 122 71 631 605 2889 1525 Fragments arranged by size: 2889 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 3367 3367 Size: 5950 Bme1580I G_kGCm'C Cuts at: 1790 2377 4011 5167 1790 Size: 587 1634 1156 2573 Fragments arranged by size: 2573 1634 1156 587 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3681 4639 442 Size: 416 53 83 399 24 2264 958 1753 Fragments arranged by size: 2264 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4610 1239 Size: 3371 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4613 882 Size: 617 3114 2219 Fragments arranged by size: 3114 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3741 493 Size: 711 2537 2702 Fragments arranged by size: 2702 2537 711 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3757 4139 4774 275 Size: 53 83 186 936 2224 382 635 1451 Fragments arranged by size: 2224 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4383 4781 5055 5202 5613 4383 Size: 398 274 147 411 4720 Fragments arranged by size: 4720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5725 Fragments arranged by size: 5725 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3044 1186 Size: 1858 4092 BseYI C'CCAG_C Cuts at: 5153 5649 5153 Size: 496 5454 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2676 3119 1163 Size: 573 940 443 3994 Fragments arranged by size: 3994 940 573 443 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2377 3049 4011 4096 5167 2377 Size: 672 962 85 1071 3160 Fragments arranged by size: 3160 1071 962 672 85 BsmI GAATG_Cn' Cuts at: 3461 3554 3461 Size: 93 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2377 3049 3269 4011 4096 5167 1391 Size: 399 587 672 220 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 672 587 399 220 85 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3305 844 Size: 2461 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2023 3837 1394 Size: 429 200 1814 3507 Fragments arranged by size: 3507 1814 429 200 BsrDI GCAATG_nn' Cuts at: 65 1248 4445 4619 65 Size: 1183 3197 174 1396 Fragments arranged by size: 3197 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4594 4781 1700 Size: 110 2784 187 2869 Fragments arranged by size: 2869 2784 187 110 BsrGI T'GTAC_A Cuts at: 96 2993 96 Size: 2897 3053 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 2031 2080 4754 2031 Size: 49 2674 3227 Fragments arranged by size: 3227 2674 49 BstEII G'GTnAC_C Cuts at: 1184 2984 1184 Size: 1800 4150 BstYI r'GATC_y Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945 Size: 1308 367 385 17 1452 86 12 346 Cuts at: 5945 1972 Size: 1977 Fragments arranged by size: 1977 1452 1308 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2858 2858 Size: 5950 BtgI C'CryG_G Cuts at: 513 1065 2629 513 Size: 552 1564 3834 Fragments arranged by size: 3834 1564 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 3466 4260 4280 3466 Size: 794 20 5136 Fragments arranged by size: 5136 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 2413 2596 3254 2413 Size: 183 658 5109 Fragments arranged by size: 5109 658 183 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 2384 2543 Size: 54 1425 50 275 18 282 272 159 Cuts at: 2543 3397 4286 5915 8 Size: 854 889 1629 43 Fragments arranged by size: 1629 1425 889 854 282 275 272 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 2229 3880 1353 Size: 675 201 1651 3423 Fragments arranged by size: 3423 1651 675 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4013 4610 5397 5665 1239 Size: 393 2381 597 787 268 1524 Fragments arranged by size: 2381 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2908 2908 Size: 5950 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 2671 3210 5097 1678 Size: 993 539 1887 2531 Fragments arranged by size: 2531 1887 993 539 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2441 2473 2776 Size: 32 135 32 773 32 64 32 303 Cuts at: 2776 2808 4591 4623 4665 4697 1373 Size: 32 1783 32 42 32 2626 Fragments arranged by size: 2626 1783 773 303 135 64 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 3294 3540 669 Size: 988 281 289 1067 246 3079 Fragments arranged by size: 3079 1067 988 289 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2019 5064 5248 1305 Size: 714 3045 184 2007 Fragments arranged by size: 3045 2007 714 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2114 2386 2545 10 Size: 54 1425 343 282 272 159 3415 Fragments arranged by size: 3415 1425 343 282 272 159 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 2652 3176 3908 4267 4426 518 Size: 648 1009 477 524 732 359 159 2042 Fragments arranged by size: 2042 1009 732 648 524 477 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 3366 4043 5191 5436 1606 Size: 459 1301 677 1148 245 2120 Fragments arranged by size: 2120 1301 1148 677 459 245 NcoI C'CATG_G Cuts at: 513 1065 2629 513 Size: 552 1564 3834 Fragments arranged by size: 3834 1564 552 NdeI CA'TA_TG Cuts at: 387 2164 387 Size: 1777 4173 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2286 3308 4853 1132 Size: 97 1057 1022 1545 2229 Fragments arranged by size: 2229 1545 1057 1022 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 2552 1108 Size: 1444 4506 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2012 2044 2506 2538 4019 4051 5532 5564 2012 Size: 32 462 32 1481 32 1481 32 2398 Fragments arranged by size: 2398 1481 1481 462 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2697 1613 Size: 1084 4866 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PstI C_TGCA'G Cuts at: 830 3302 830 Size: 2472 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 2381 4198 5910 2381 Size: 1817 1712 2421 Fragments arranged by size: 2421 1817 1712 SfcI C'TryA_G Cuts at: 826 1045 2650 3298 4433 5114 5305 826 Size: 219 1605 648 1135 681 191 1471 Fragments arranged by size: 1605 1471 1135 681 648 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2629 3389 513 Size: 552 1564 760 3074 Fragments arranged by size: 3074 1564 760 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3003 3977 3994 4147 4332 4777 325 Size: 1255 1423 974 17 153 185 445 1498 Fragments arranged by size: 1498 1423 1255 974 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 2379 2470 Size: 274 80 33 51 1443 90 312 91 Cuts at: 2470 2993 4196 5908 96 Size: 523 1203 1712 138 Fragments arranged by size: 1712 1443 1203 523 312 274 138 91 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2415 2447 5910 5942 2415 Size: 32 3463 32 2423 Fragments arranged by size: 3463 2423 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 2962 3286 2962 Size: 324 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 4079 4079 Size: 5950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI