################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HpaI KasI KpnI MaeI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 620 GGCGTC G[GA]^CG[CT]C AflIII 262 ACGTGT A^C[GA][CT]GT AhdI 348 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 485 GGATC GGATC(4/5) AseI 421 ATTAAT AT^TAAT AsnI 421 ATTAAT AT^TAAT AspEI 348 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 572 GGTCC G^G[AT]CC BanI 308 GGTGCC G^G[CT][GA]CC BcgI 625 CGAGCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 160 CTGGAG CTGGAG(16/14) BsaAI 261 TACGTG [CT]AC^GT[GA] BsaHI 620 GGCGTC G[GA]^CG[CT]C BsmFI 573 GTCCC GGGAC(10/14) BspMI 316 ACCTGC ACCTGC(4/8) BssHII 449 GCGCGC G^CGCGC BstAPI 37 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cac8I 449 GCGCGC GC[ACGT]^[ACGT]GC DpnII 486 GATC ^GATC DraIII 586 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 576 CCGCGG C^C[GA][CT]GG Eam1105I 348 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 575 CCCGC CCCGC(4/6) HaeIII 594 GGCC GG^CC HapII 375 CCGG C^CGG Hin1I 620 GGCGTC G[GA]^CG[CT]C HincII 523 GTTGAC GT[CT]^[GA]AC HindII 523 GTTGAC GT[CT]^[GA]AC HindIII 359 AAGCTT A^AGCTT HpaII 375 CCGG C^CGG KspI 576 CCGCGG CCGC^GG MaeIII 584 GTCAC ^GT[ACGT]AC MboI 486 GATC ^GATC MseI 422 TTAA T^TAA MspI 375 CCGG C^CGG NdeII 486 GATC ^GATC PleI 223 GAGTC GAGTC(4/5) PshBI 421 ATTAAT AT^TAAT PstI 504 CTGCAG CTGCA^G SacII 576 CCGCGG CCGC^GG Sau3AI 486 GATC ^GATC SexAI 395 ACCTGGT A^CC[AT]GGT SspI 418 AATATT AAT^ATT TfiI 431 GAATC G^A[AT]TC Tru9I 422 TTAA T^TAA Tsp45I 584 GTCAC ^GT[GC]AC Tsp509I 424 AATT ^AATT ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 450 CGCG CG^CG 577 CGCG AfaI 549 GTAC GT^AC 602 GTAC AluI 158 AGCT AG^CT 360 AGCT AlwNI 38 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG 531 CAGCCGCTG AocI 106 CCTGAGG CC^T[ACGT]AGG 268 CCTGAGG AvaI 495 CTCGGG C^[CT]CG[GA]G 605 CCCGAG BanII 180 GGGCTC G[GA]GC[CT]^C 626 GAGCCC BbsI 641 GAAGAC GAAGAC(2/6) 341 GTCTTC BmyI 180 GGGCTC G[AGT]GC[ACT]^C 626 GAGCCC BpuAI 641 GAAGAC GAAGAC(2/6) 341 GTCTTC BsaI 79 GAGACC GGTCTC(1/5) 142 GAGACC BsiYI 146 CCCCTTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 147 CCCTTGCAGGG BslI 146 CCCCTTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 147 CCCTTGCAGGG BsoBI 495 CTCGGG C^[CT]CG[GA]G 605 CCCGAG Bsp1286I 180 GGGCTC G[AGT]GC[ACT]^C 626 GAGCCC BstUI 450 CGCG CG^CG 577 CGCG Bsu36I 106 CCTGAGG CC^T[ACGT]AGG 268 CCTGAGG CfoI 449 GCGC GCG^C 451 GCGC Cfr13I 572 GGTCC G^G[ACGT]CC 594 GGCCC Eco81I 106 CCTGAGG CC^T[ACGT]AGG 268 CCTGAGG HhaI 449 GCGC GCG^C 451 GCGC HinP1I 449 GCGC G^CGC 451 GCGC HinfI 223 GAGTC G^A[ACGT]TC 431 GAATC HphI 333 GGTGA GGTGA(8/7) 561 TCACC MaeII 262 ACGT A^CGT 349 ACGT MspA1I 534 CCGCTG C[AC]G^C[GT]G 576 CCGCGG MvnI 450 CGCG CG^CG 577 CGCG NlaIV 308 GGTGCC GG[ACGT]^[ACGT]CC 572 GGTCCC RsaI 549 GTAC GT^AC 602 GTAC SapI 22 GCTCTTC GCTCTTC(1/4) 46 GCTCTTC Sau96I 572 GGTCC G^G[ACGT]CC 594 GGCCC SfaNI 196 GATGC GCATC(5/9) 299 GCATC SfcI 384 CTACAG C^T[GA][CT]AG 504 CTGCAG TaqI 429 TCGA T^CGA 624 TCGA TthHB8I 429 TCGA T^CGA 624 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI BbvI BsaJI BseRI BsmAI BsrI BssKI BstF5I BstNI DdeI EarI EcoRII Fnu4HI FokI HgaI ItaI Ksp632I MboII MnlI MvaI MwoI ScrFI TseI TspRI