################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeIII HapII HgaI HincII HindII HindIII HpaI HpaII KpnI KspI MamI MluI MluNI MroI MscI MspI MvnI MwoI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau96I SbfI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TspRI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AciI 317 GCGG CCGC(-3/-1) AcyI 179 GGCGCC G[GA]^CG[CT]C AspHI 65 GAGCTC G[AT]GC[AT]^C AvaI 680 CTCGAG C^[CT]CG[GA]G BamHI 167 GGATCC G^GATCC BanI 179 GGCGCC G^G[CT][GA]CC BanII 65 GAGCTC G[GA]GC[CT]^C BbeI 179 GGCGCC GGCGC^C BpmI 257 CTGGAG CTGGAG(16/14) BsaHI 179 GGCGCC G[GA]^CG[CT]C BsiHKAI 65 GAGCTC G[AT]GC[AT]^C BsiYI 40 CCTCAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 40 CCTCAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 242 GAGACG CGTCTC(1/5) BsoBI 680 CTCGAG C^[CT]CG[GA]G BsrDI 333 CATTGC GCAATG(2/0) BstF5I 131 CATCC GGATG(2/0) CfoI 180 GCGC GCG^C DsaI 162 CCACGG C^C[GA][CT]GG EarI 769 GAAGAG CTCTTC(1/4) FokI 131 CATCC GGATG(9/13) HaeII 179 GGCGCC [GA]GCGC^[CT] HhaI 180 GCGC GCG^C Hin1I 179 GGCGCC G[GA]^CG[CT]C HinP1I 180 GCGC G^CGC KasI 179 GGCGCC G^GCGCC Ksp632I 769 GAAGAG CTCTTC(1/4) MaeII 25 ACGT A^CGT MfeI 637 CAATTG C^AATTG MslI 276 CACTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 315 CAGCGG C[AC]G^C[GT]G MunI 637 CAATTG C^AATTG NarI 179 GGCGCC GG^CGCC NlaIII 405 CATG CATG^ PaeR7I 680 CTCGAG C^TCGAG SacI 65 GAGCTC GAGCT^C ScaI 421 AGTACT AGT^ACT SexAI 45 ACCTGGT A^CC[AT]GGT SfaNI 514 GCATC GCATC(5/9) SfoI 179 GGCGCC GGC^GCC SmlI 680 CTCGAG C^T[CT][GA]AG TaqI 681 TCGA T^CGA TfiI 7 GATTC G^A[AT]TC TthHB8I 681 TCGA T^CGA XcmI 488 CCAGCAATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 680 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BfaI 172 CTAG C^TAG 311 CTAG BmyI 65 GAGCTC G[AGT]GC[ACT]^C 484 GTGCCC BsaJI 122 CCCAGG C^C[ACGT][ACGT]GG 162 CCACGG BseRI 147 CTCCTC GAGGAG(10/8) 115 GAGGAG BsmAI 31 GAGAC GTCTC(1/5) 242 GAGAC BsmFI 387 GGGAC GGGAC(10/14) 120 GTCCC Bsp1286I 65 GAGCTC G[AGT]GC[ACT]^C 484 GTGCCC HphI 582 GGTGA GGTGA(8/7) 605 TCACC MaeI 172 CTAG C^TAG 311 CTAG MaeIII 274 GTCAC ^GT[ACGT]AC 598 GTGAC Tsp45I 274 GTCAC ^GT[GC]AC 598 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BssKI BstNI BstYI DdeI DpnII EcoRII Fnu4HI HinfI ItaI MboI MboII MflI MnlI MseI MvaI NdeII NlaIV PleI RsaI Sau3AI ScrFI Tru9I TseI Tsp509I XhoII