Restriction map for
pFN21AB7558
(Linear) (Six-base) MAPSORT of: KIBB7558.seq Check: 7179 from: 1 to: 813
KIBB7558.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 376 430 539 813
Size: 239 137 54 109 274
Fragments arranged by size:
274 239 137 109 54
ApoI r'AATT_y
Cuts at: 0 197 252 411 813
Size: 197 55 159 402
Fragments arranged by size:
402 197 159 55
AvaI C'yCGr_G
Cuts at: 0 680 813
Size: 680 133
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3 36 813
Size: 3 33 777
Fragments arranged by size:
777 33 3
BamHI G'GATC_C
Cuts at: 0 167 813
Size: 167 646
BanI G'GyrC_C
Cuts at: 0 179 813
Size: 179 634
BanII G_rGCy'C
Cuts at: 0 69 813
Size: 69 744
BbeI G_GCGC'C
Cuts at: 0 183 813
Size: 183 630
Bme1580I G_kGCm'C
Cuts at: 0 488 813
Size: 488 325
BmgBI CAC'GTC
Cuts at: 0 26 813
Size: 26 787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 813
Size: 278 535
BsaHI Gr'CG_yC
Cuts at: 0 180 813
Size: 180 633
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 138 813
Size: 130 8 675
Fragments arranged by size:
675 130 8
BseYI C'CCAG_C
Cuts at: 0 487 813
Size: 487 326
BsiHKAI G_wGCw'C
Cuts at: 0 69 813
Size: 69 744
BsmBI CGTCTCn'nnnn_
Cuts at: 0 236 813
Size: 236 577
Bsp1286I G_dGCh'C
Cuts at: 0 69 488 813
Size: 69 419 325
Fragments arranged by size:
419 325 69
BsrDI GCAATG_nn'
Cuts at: 0 332 813
Size: 332 481
BstYI r'GATC_y
Cuts at: 0 167 539 789 813
Size: 167 372 250 24
Fragments arranged by size:
372 250 167 24
BtgI C'CryG_G
Cuts at: 0 162 813
Size: 162 651
EarI CTCTTCn'nnn_
Cuts at: 0 764 813
Size: 764 49
EcoICRI GAG'CTC
Cuts at: 0 67 813
Size: 67 746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 278 376 430 539 813
Size: 239 39 98 54 109 274
Fragments arranged by size:
274 239 109 98 54 39
HaeII r_GCGC'y
Cuts at: 0 183 813
Size: 183 630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 24 56 576 608 722 754 813
Size: 24 32 520 32 114 32 59
Fragments arranged by size:
520 114 59 32 32 32 24
KasI G'GCGC_C
Cuts at: 0 179 813
Size: 179 634
MfeI C'AATT_G
Cuts at: 0 637 813
Size: 637 176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 316 813
Size: 158 158 497
Fragments arranged by size:
497 158 158
MslI CAynn'nnrTG
Cuts at: 0 280 813
Size: 280 533
MspA1I CmG'CkG
Cuts at: 0 317 813
Size: 317 496
NarI GG'CG_CC
Cuts at: 0 180 813
Size: 180 633
PfoI T'CCnGG_A
Cuts at: 0 740 813
Size: 740 73
SacI G_AGCT'C
Cuts at: 0 69 813
Size: 69 744
ScaI AGT'ACT
Cuts at: 0 423 813
Size: 423 390
SexAI A'CCwGG_T
Cuts at: 0 45 813
Size: 45 768
SfoI GGC'GCC
Cuts at: 0 181 813
Size: 181 632
SmlI C'TyrA_G
Cuts at: 0 680 813
Size: 680 133
TatI w'GTAC_w
Cuts at: 0 381 421 813
Size: 381 40 392
Fragments arranged by size:
392 381 40
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 495 813
Size: 495 318
XhoI C'TCGA_G
Cuts at: 0 680 813
Size: 680 133
Enzymes that do cut:
AcuI ApoI AvaI BaeI BamHI BanI BanII BbeI
Bme1580I BmgBI BpmI BsaHI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BsrDI BstYI BtgI EarI EcoICRI Eco57MI HaeII
Hin4I KasI MfeI MmeI MslI MspA1I NarI PfoI
SacI ScaI SexAI SfoI SmlI TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MluI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI Tth111I
XbaI XmaI XmnI ZraI