(Circular) (Six-base) MAPSORT of: pFN21AE0324.seq Check: 2648 from: 1 to: 6559 pFN21AE0324 6559 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4369 278 Size: 53 83 186 936 2833 2468 Fragments arranged by size: 2833 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2417 3902 1937 Size: 480 1485 4594 Fragments arranged by size: 4594 1485 480 Acc65I G'GTAC_C Cuts at: 3880 3880 Size: 6559 AclI AA'CG_TT Cuts at: 1563 4686 5059 1563 Size: 3123 373 3063 Fragments arranged by size: 3123 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2480 3177 3293 3474 4622 6006 6274 1632 Size: 848 697 116 181 1148 1384 268 1917 Fragments arranged by size: 1917 1384 1148 848 697 268 181 116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6362 AflIII A'CryG_T Cuts at: 1203 5458 6435 1203 Size: 4255 977 1327 Fragments arranged by size: 4255 1327 977 AgeI A'CCGG_T Cuts at: 5390 5390 Size: 6559 AhdI GACnn_n'nnGTC Cuts at: 5288 5288 Size: 6559 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6559 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6559 AlwNI CAG_nnn'CTG Cuts at: 4302 5874 4302 Size: 1572 4987 ApaI G_GGCC'C Cuts at: 2520 2520 Size: 6559 ApaLI G'TGCA_C Cuts at: 4616 5772 4616 Size: 1156 5403 ApoI r'AATT_y Cuts at: 1493 2087 3348 3443 3868 4094 4246 6355 1493 Size: 594 1261 95 425 226 152 2109 1697 Fragments arranged by size: 2109 1697 1261 594 425 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 5113 160 Size: 872 4081 1606 Fragments arranged by size: 4081 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6559 AvaI C'yCGr_G Cuts at: 1870 1943 2109 3884 1870 Size: 73 166 1775 4545 Fragments arranged by size: 4545 1775 166 73 BamHI G'GATC_C Cuts at: 2022 3889 4256 2022 Size: 1867 367 4325 Fragments arranged by size: 4325 1867 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3132 3880 5335 618 Size: 325 217 627 1345 748 1455 1842 Fragments arranged by size: 1842 1455 1345 748 627 325 217 BanII G_rGCy'C Cuts at: 1391 2478 2520 3773 3878 1391 Size: 1087 42 1253 105 4072 Fragments arranged by size: 4072 1253 1087 105 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 928 Size: 971 161 5427 Fragments arranged by size: 5427 971 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4750 4784 1903 Size: 34 2813 34 3678 Fragments arranged by size: 3678 2813 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3750 4451 5661 3750 Size: 701 1210 4648 Fragments arranged by size: 4648 1210 701 BclI T'GATC_A Cuts at: 1548 2593 1548 Size: 1045 5514 BfrBI ATG'CAT Cuts at: 2918 2918 Size: 6559 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5170 136 Size: 107 122 71 631 605 3498 1525 Fragments arranged by size: 3498 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6554 6554 Size: 6559 BlpI GC'TnA_GC Cuts at: 3976 3976 Size: 6559 Bme1580I G_kGCm'C Cuts at: 1790 2520 4620 5776 1790 Size: 730 2100 1156 2573 Fragments arranged by size: 2573 2100 1156 730 BmgBI CAC'GTC Cuts at: 1318 2017 1318 Size: 699 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3091 3693 4290 Size: 416 53 83 399 24 1674 602 597 Cuts at: 4290 5248 442 Size: 958 1753 Fragments arranged by size: 1753 1674 958 602 597 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6559 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2702 5219 1239 Size: 1463 2517 2579 Fragments arranged by size: 2579 2517 1463 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2658 3171 3749 4684 5549 5847 2658 Size: 513 578 935 865 298 3370 Fragments arranged by size: 3370 935 865 578 513 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2384 5222 882 Size: 617 885 2838 2219 Fragments arranged by size: 2838 2219 885 617 BsaAI yAC'GTr Cuts at: 493 1204 3748 4350 493 Size: 711 2544 602 2702 Fragments arranged by size: 2702 2544 711 602 BsaBI GATnn'nnATC Cuts at: 6553 6553 Size: 6559 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4366 4748 5383 275 Size: 53 83 186 936 2833 382 635 1451 Fragments arranged by size: 2833 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4992 5390 5664 5811 6222 4992 Size: 398 274 147 411 5329 Fragments arranged by size: 5329 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6334 Fragments arranged by size: 6334 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2549 2661 1186 Size: 1363 112 5084 Fragments arranged by size: 5084 1363 112 BseYI C'CCAG_C Cuts at: 5762 6258 5762 Size: 496 6063 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5986 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4009 4770 5798 1540 Size: 275 186 2008 761 1028 2301 Fragments arranged by size: 2301 2008 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2478 3773 4620 4705 5776 2478 Size: 1295 847 85 1071 3261 Fragments arranged by size: 3261 1295 1071 847 85 BsmI GAATG_Cn' Cuts at: 2266 4070 4163 2266 Size: 1804 93 4662 Fragments arranged by size: 4662 1804 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2478 2520 3773 3878 4620 4705 5776 Size: 399 688 42 1253 105 742 85 1071 Cuts at: 5776 1391 Size: 2174 Fragments arranged by size: 2174 1253 1071 742 688 399 105 85 42 BspEI T'CCGG_A Cuts at: 6222 6222 Size: 6559 BspHI T'CATG_A Cuts at: 4448 6178 4448 Size: 1730 4829 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3237 3603 3914 844 Size: 2393 366 311 3489 Fragments arranged by size: 3489 2393 366 311 BsrBI CCG'CTC Cuts at: 1394 1823 4446 1394 Size: 429 2623 3507 Fragments arranged by size: 3507 2623 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5054 5228 65 Size: 1183 3806 174 1396 Fragments arranged by size: 3806 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5203 5390 1700 Size: 110 3393 187 2869 Fragments arranged by size: 3393 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3124 3834 96 Size: 3028 710 2821 Fragments arranged by size: 3028 2821 710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6559 BssSI C'ACGA_G Cuts at: 4619 5631 4619 Size: 1012 5547 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6559 BstBI TT'CG_AA Cuts at: 5363 5363 Size: 6559 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6559 BstYI r'GATC_y Cuts at: 1972 2022 3889 4256 4641 4658 6110 6196 6208 Size: 50 1867 367 385 17 1452 86 12 Cuts at: 6208 6554 1972 Size: 346 1977 Fragments arranged by size: 1977 1867 1452 385 367 346 86 50 17 12 BstZ17I GTA'TAC Cuts at: 2418 2418 Size: 6559 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6007 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5820 Fragments arranged by size: 5820 683 56 BtsI GCAGTG_nn' Cuts at: 4075 4869 4889 4075 Size: 794 20 5745 Fragments arranged by size: 5745 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6559 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6524 DraI TTT'AAA Cuts at: 2584 3004 3863 2584 Size: 420 859 5280 Fragments arranged by size: 5280 859 420 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5566 809 Size: 507 4250 1802 Fragments arranged by size: 4250 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3732 4006 4895 Size: 54 1425 50 275 18 1902 274 889 Cuts at: 4895 6524 8 Size: 1629 43 Fragments arranged by size: 1902 1629 1425 889 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4006 1537 Size: 275 2194 4090 Fragments arranged by size: 4090 2194 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 3321 4489 1353 Size: 738 1230 1168 3423 Fragments arranged by size: 3423 1230 1168 738 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5146 5520 5666 387 Size: 1064 138 3557 374 146 1280 Fragments arranged by size: 3557 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2476 3771 2476 Size: 1295 5264 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2480 2702 3177 3293 3474 4622 5219 Size: 393 848 222 475 116 181 1148 597 Cuts at: 5219 6006 6274 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1148 848 787 597 475 393 268 222 181 116 EcoO109I rG'GnC_Cy Cuts at: 2516 2517 2516 Size: 1 6558 EcoRI G'AATT_C Cuts at: 3868 4246 3868 Size: 378 6181 EcoRV GAT'ATC Cuts at: 3430 3709 3430 Size: 279 6280 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2519 3562 3727 2519 Size: 1043 165 5351 Fragments arranged by size: 5351 1043 165 FspI TGC'GCA Cuts at: 5065 5065 Size: 6559 HaeII r_GCGC'y Cuts at: 1678 5706 1678 Size: 4028 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2056 2088 3167 3199 3350 Size: 32 135 32 484 32 1079 32 151 Cuts at: 3350 3382 5200 5232 5274 5306 1373 Size: 32 1818 32 42 32 2626 Fragments arranged by size: 2626 1818 1079 484 151 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2257 2742 3903 4149 669 Size: 988 281 319 485 1161 246 3079 Fragments arranged by size: 3079 1161 988 485 319 281 246 HindIII A'AGCT_T Cuts at: 748 3189 748 Size: 2441 4118 HpaI GTT'AAC Cuts at: 2257 4149 2257 Size: 1892 4667 KpnI G_GTAC'C Cuts at: 3884 3884 Size: 6559 MfeI C'AATT_G Cuts at: 4158 4158 Size: 6559 MluI A'CGCG_T Cuts at: 6435 6435 Size: 6559 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2183 5673 5857 1305 Size: 878 3490 184 2007 Fragments arranged by size: 3490 2007 878 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3734 10 Size: 54 1425 343 1902 2835 Fragments arranged by size: 2835 1902 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2715 4517 4876 5035 518 Size: 648 1549 1802 359 159 2042 Fragments arranged by size: 2042 1802 1549 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2135 3130 3197 3975 4652 5800 6045 1606 Size: 529 995 67 778 677 1148 245 2120 Fragments arranged by size: 2120 1148 995 778 677 529 245 67 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6007 NdeI CA'TA_TG Cuts at: 387 387 Size: 6559 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6559 NotI GC'GGCC_GC Cuts at: 4006 4006 Size: 6559 NsiI A_TGCA'T Cuts at: 2920 2920 Size: 6559 NspI r_CATG'y Cuts at: 1132 1229 3917 5462 1132 Size: 97 2688 1545 2229 Fragments arranged by size: 2688 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6559 PciI A'CATG_T Cuts at: 5458 5458 Size: 6559 PflMI CCAn_nnn'nTGG Cuts at: 1108 3780 1108 Size: 2672 3887 PmeI GTTT'AAAC Cuts at: 3863 3863 Size: 6559 PmlI CAC'GTG Cuts at: 3748 3748 Size: 6559 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4628 4660 6141 6173 4628 Size: 32 1481 32 5014 Fragments arranged by size: 5014 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6559 PsiI TTA'TAA Cuts at: 4129 4129 Size: 6559 PspOMI G'GGCC_C Cuts at: 2516 2516 Size: 6559 PstI C_TGCA'G Cuts at: 830 3234 3644 3911 830 Size: 2404 410 267 3478 Fragments arranged by size: 3478 2404 410 267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6559 PvuII CAG'CTG Cuts at: 2135 3197 2135 Size: 1062 5497 SacI G_AGCT'C Cuts at: 2478 3773 2478 Size: 1295 5264 SalI G'TCGA_C Cuts at: 1936 3901 1936 Size: 1965 4594 SapI GCTCTTCn'nnn_ Cuts at: 3321 3321 Size: 6559 SbfI CC_TGCA'GG Cuts at: 3911 3911 Size: 6559 ScaI AGT'ACT Cuts at: 4807 6519 4807 Size: 1712 4847 SfcI C'TryA_G Cuts at: 826 1045 2511 3230 3640 3907 5042 5723 5914 Size: 219 1466 719 410 267 1135 681 191 Cuts at: 5914 826 Size: 1471 Fragments arranged by size: 1471 1466 1135 719 681 410 267 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6559 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6559 SmaI CCC'GGG Cuts at: 1872 2111 3886 1872 Size: 239 1775 4545 Fragments arranged by size: 4545 1775 239 SmlI C'TyrA_G Cuts at: 820 1017 1943 2673 3186 3764 4663 5564 5826 Size: 197 926 730 513 578 899 901 262 Cuts at: 5826 820 Size: 1553 Fragments arranged by size: 1553 926 901 899 730 578 513 262 197 SnaBI TAC'GTA Cuts at: 493 4350 493 Size: 3857 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6559 SphI G_CATG'C Cuts at: 1132 3917 1132 Size: 2785 3774 SspI AAT'ATT Cuts at: 5 52 2346 2861 3143 3168 4483 5 Size: 47 2294 515 282 25 1315 2081 Fragments arranged by size: 2294 2081 1315 515 282 47 25 StuI AGG'CCT Cuts at: 1517 1517 Size: 6559 StyI C'CwwG_G Cuts at: 513 1065 3023 3998 513 Size: 552 1958 975 3074 Fragments arranged by size: 3074 1958 975 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4586 4603 4756 4941 5386 325 Size: 1255 3006 17 153 185 445 1498 Fragments arranged by size: 3006 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2523 3124 3834 Size: 274 80 33 51 1443 546 601 710 Cuts at: 3834 4805 6517 96 Size: 971 1712 138 Fragments arranged by size: 1712 1443 971 710 601 546 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2763 2795 6519 6551 2763 Size: 32 3724 32 2771 Fragments arranged by size: 3724 2771 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6559 XbaI T'CTAG_A Cuts at: 3895 3895 Size: 6559 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6559 XmaI C'CCGG_G Cuts at: 1870 2109 3884 1870 Size: 239 1775 4545 Fragments arranged by size: 4545 1775 239 XmnI GAAnn'nnTTC Cuts at: 2088 2100 2205 4688 2088 Size: 12 105 2483 3959 Fragments arranged by size: 3959 2483 105 12 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4367 276 Size: 53 83 186 936 2833 2468 Fragments arranged by size: 2833 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI